A new fluorescent-avidin–based method for quantifying basophil activation in whole blood

• Published in: Journal of allergy and clinical immunology Vol. 140; no. 4; pp. 1202 - 1206.e3
• Main Authors: Mukai, Kaori, Chinthrajah, R. Sharon, Nadeau, Kari C., Tsai, Mindy, Gaudenzio, Nicolas, Galli, Stephen J.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01.10.2017; Elsevier Limited; Elsevier
• Subjects: Adolescent; Adult; Allergy and Immunology; Avidin; Basophils - immunology; Blood & organ donations; Child; Child, Preschool; Desensitization, Immunologic; Double-Blind Method; Female; Flow cytometry; Fluorescent Dyes; Food allergies; Human health and pathology; Humans; Immunologic Tests; Immunotherapy; Infectious diseases; Life Sciences; Male; Middle Aged; Peanut Hypersensitivity - immunology; Peanut Hypersensitivity - therapy; Young Adult; BATs; CD63; peanut allergy; avidin; AlexaFluor488-labeled avidin; flow cytometry; Av.A488; mast cells; MCs; Basophil; Basophil activation tests

We report herein a simple new fluorescent-avidin-based method to detect activated basophils in the whole blood of normal or allergic subjects, and compare this method to a basophil activation test based on detection of CD63.