A new fluorescent-avidin–based method for quantifying basophil activation in whole blood

We report herein a simple new fluorescent-avidin-based method to detect activated basophils in the whole blood of normal or allergic subjects, and compare this method to a basophil activation test based on detection of CD63.

Saved in:
Bibliographic Details
Published inJournal of allergy and clinical immunology Vol. 140; no. 4; pp. 1202 - 1206.e3
Main Authors Mukai, Kaori, Chinthrajah, R. Sharon, Nadeau, Kari C., Tsai, Mindy, Gaudenzio, Nicolas, Galli, Stephen J.
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 01.10.2017
Elsevier Limited
Elsevier
Subjects
Adolescent
Adult
Allergy and Immunology
Avidin
Basophils - immunology
Blood & organ donations
Child
Child, Preschool
Desensitization, Immunologic
Double-Blind Method
Female
Flow cytometry
Fluorescent Dyes
Food allergies
Human health and pathology
Humans
Immunologic Tests
Immunotherapy
Infectious diseases
Life Sciences
Male
Middle Aged
Peanut Hypersensitivity - immunology
Peanut Hypersensitivity - therapy
Young Adult
BATs
CD63
peanut allergy
avidin
AlexaFluor488-labeled avidin
flow cytometry
Av.A488
mast cells
MCs
Basophil
Basophil activation tests
Online AccessGet full text

Cover

More Information
Summary:We report herein a simple new fluorescent-avidin-based method to detect activated basophils in the whole blood of normal or allergic subjects, and compare this method to a basophil activation test based on detection of CD63.
Bibliography:SourceType-Scholarly Journals-1
ObjectType-Correspondence-2
content type line 14
ObjectType-Letter to the Editor-1
ObjectType-Correspondence-1
content type line 23
ObjectType-Undefined-2
ObjectType-Article-3
PMCID: PMC5632583
Equal contribution
ISSN:0091-6749
1097-6825
1097-6825
DOI:10.1016/j.jaci.2017.03.052