SARS-CoV-2 Virus Culture and Subgenomic RNA for Respiratory Specimens from Patients with Mild Coronavirus Disease
We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory syndrome coronavirus 2 and subgenomic RNA were rarely detectable beyond 8 days after onset of illness. However, virus RNA was detectable for...
Saved in:
| Published in | Emerging infectious diseases Vol. 26; no. 11; pp. 2701 - 2704 |
|---|---|
| Main Authors | , , , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
U.S. National Center for Infectious Diseases
01.11.2020
Centers for Disease Control and Prevention |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory syndrome coronavirus 2 and subgenomic RNA were rarely detectable beyond 8 days after onset of illness. However, virus RNA was detectable for many weeks by reverse transcription PCR. |
|---|---|
| AbstractList | We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory syndrome coronavirus 2 and subgenomic RNA were rarely detectable beyond 8 days after onset of illness. However, virus RNA was detectable for many weeks by reverse transcription PCR. We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory syndrome coronavirus 2 and subgenomic RNA were rarely detectable beyond 8 days after onset of illness. However, virus RNA was detectable for many weeks by reverse transcription PCR.We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory syndrome coronavirus 2 and subgenomic RNA were rarely detectable beyond 8 days after onset of illness. However, virus RNA was detectable for many weeks by reverse transcription PCR. |
| Audience | Professional Academic |
| Author | Tsang, Dominic N.C. Leung, Yonna W.Y. Chu, Daniel K.W. Peiris, Malik Perera, Ranawaka A.P.M. Tso, Eugene Fung, Kitty Tsang, Owen T.Y. Poon, Leo L.M. Chin, Alex W.H. Cheng, Samuel M.S. Chuang, Vivien W.M. |
| Author_xml | – sequence: 1 givenname: Ranawaka A.P.M. surname: Perera fullname: Perera, Ranawaka A.P.M. – sequence: 2 givenname: Eugene surname: Tso fullname: Tso, Eugene – sequence: 3 givenname: Owen T.Y. surname: Tsang fullname: Tsang, Owen T.Y. – sequence: 4 givenname: Dominic N.C. surname: Tsang fullname: Tsang, Dominic N.C. – sequence: 5 givenname: Kitty surname: Fung fullname: Fung, Kitty – sequence: 6 givenname: Yonna W.Y. surname: Leung fullname: Leung, Yonna W.Y. – sequence: 7 givenname: Alex W.H. surname: Chin fullname: Chin, Alex W.H. – sequence: 8 givenname: Daniel K.W. surname: Chu fullname: Chu, Daniel K.W. – sequence: 9 givenname: Samuel M.S. surname: Cheng fullname: Cheng, Samuel M.S. – sequence: 10 givenname: Leo L.M. surname: Poon fullname: Poon, Leo L.M. – sequence: 11 givenname: Vivien W.M. surname: Chuang fullname: Chuang, Vivien W.M. – sequence: 12 givenname: Malik surname: Peiris fullname: Peiris, Malik |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/32749957$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNk9uPEyEUxidmjXvRVx8NiYnRh6kwV3jZpKm3JqtrWu0rYZgzLc0MdIFZ3f9euu1extTE8ACB3_fB4ZxzGh1poyGKXhI8ShNM3oOqk4KQUYLThLAn0QnBFMcFztnR_TrDx9Gpc2uMSZCwZ9FxmpQZY3l5El3Nx7N5PDGLOEELZXuHJn3rewtI6BrN-2oJ2nRKotm3MWqMRTNwG2WFN_YGzTcgVQfaocaaDn0XXoH2Dv1SfoW-qrZGE2ONFte3xh-UA-HgefS0Ea2DF_v5LPr56eOPyZf44vLzdDK-iGVBqY9lliZF2VRAJKGS1KlkVZ1IYCJtMpIyJgCzKstpVYUDmdGqSFhTlyWlZVPjIj2Lpjvf2og131jVCXvDjVD8dsPYJRfWK9kCJzRoQdKypjLLpaR1inMiqoSKIhWMBq_zndemrzqoZYjSinZgOjzRasWX5pqXOaV5kgWDt3sDa656cJ53ykloW6HB9I4HBBc5LfI0oK__Qtemtzp8VaCKgpFt4h6opQgBKN2YcK_cmvJxkSWY5nmOAxUfoEJKITwyFFKjwvaAHx3gw6gh1MBBwbuBIDAefvul6J3j0_ns_9nLxZB984hdgWj9ypm298poNwRfPc7MfUruKjwA2Q6Q1jhnoeFSebH1CaGplhPMt43E943Ed4308BP3sjvnfwj-AIKbGn0 |
| CitedBy_id | crossref_primary_10_1016_j_heliyon_2025_e42503 crossref_primary_10_1093_infdis_jiaa737 crossref_primary_10_1128_jvi_01034_22 crossref_primary_10_1016_j_cossms_2021_100966 crossref_primary_10_1016_j_idnow_2022_12_002 crossref_primary_10_1016_j_jcv_2021_104817 crossref_primary_10_1371_journal_pone_0285704 crossref_primary_10_1093_infdis_jiac198 crossref_primary_10_3390_v14061294 crossref_primary_10_2807_1560_7917_ES_2020_25_32_2001483 crossref_primary_10_1093_ofid_ofac619 crossref_primary_10_1016_j_isci_2022_105178 crossref_primary_10_1080_1354750X_2021_1876769 crossref_primary_10_1128_JCM_02769_20 crossref_primary_10_1186_s12879_022_07185_w crossref_primary_10_1016_j_resinv_2025_03_004 crossref_primary_10_3201_eid2712_211621 crossref_primary_10_3390_diagnostics11061091 crossref_primary_10_1007_s00705_022_05392_z crossref_primary_10_1016_j_ebiom_2021_103355 crossref_primary_10_1080_23744235_2022_2035428 crossref_primary_10_3389_fmicb_2021_749149 crossref_primary_10_1016_j_jcv_2022_105247 crossref_primary_10_1016_S2666_5247_21_00216_0 crossref_primary_10_1016_j_ijid_2023_05_008 crossref_primary_10_1080_08830185_2021_1967949 crossref_primary_10_3346_jkms_2022_37_e106 crossref_primary_10_23749_mdl_v113i2_12724 crossref_primary_10_1093_cid_ciaa1280 crossref_primary_10_1093_pubmed_fdab145 crossref_primary_10_1016_j_micres_2022_127185 crossref_primary_10_1128_mmbr_00026_21 crossref_primary_10_3390_microorganisms9081738 crossref_primary_10_1002_jmv_26970 crossref_primary_10_1016_j_jcv_2021_104945 crossref_primary_10_1093_infdis_jiab394 crossref_primary_10_1002_jmv_26610 crossref_primary_10_3389_fdgth_2021_655745 crossref_primary_10_3390_v15051052 crossref_primary_10_1089_vbz_2021_0080 crossref_primary_10_1017_ice_2021_132 crossref_primary_10_1038_s41467_020_19883_7 crossref_primary_10_1080_14737159_2021_1962709 crossref_primary_10_1016_j_jcv_2023_105499 crossref_primary_10_1128_spectrum_00182_22 crossref_primary_10_3390_v14081608 crossref_primary_10_1016_j_cll_2022_02_004 crossref_primary_10_7326_M20_5008 crossref_primary_10_1016_j_cll_2022_02_007 crossref_primary_10_7554_eLife_65774 crossref_primary_10_1093_jalm_jfae010 crossref_primary_10_1002_emp2_12605 crossref_primary_10_1073_pnas_2111477118 crossref_primary_10_1002_pbc_29277 crossref_primary_10_1016_j_ijid_2021_02_005 crossref_primary_10_1002_jmv_28369 crossref_primary_10_3390_curroncol28010083 crossref_primary_10_29328_journal_ijcv_1001041 crossref_primary_10_3389_fmicb_2021_718497 crossref_primary_10_1128_cmr_00072_23 crossref_primary_10_1016_j_cmi_2021_08_009 crossref_primary_10_17816_EID83663 crossref_primary_10_1186_s12879_021_06534_5 crossref_primary_10_1016_j_ijid_2021_05_020 crossref_primary_10_1080_03007995_2021_1914943 crossref_primary_10_1093_cid_ciaa1616 crossref_primary_10_5812_archcid_128040 crossref_primary_10_1016_j_ebiom_2022_103997 crossref_primary_10_1093_pnasnexus_pgad033 crossref_primary_10_1515_cclm_2022_0661 crossref_primary_10_1016_j_bjid_2021_101632 crossref_primary_10_1093_infdis_jiab415 crossref_primary_10_1016_j_ymeth_2021_04_011 crossref_primary_10_3390_v14020366 crossref_primary_10_7554_eLife_66857 crossref_primary_10_1128_JCM_02277_20 crossref_primary_10_1128_JCM_01609_21 crossref_primary_10_1016_j_jviromet_2024_114912 crossref_primary_10_4103_jmms_jmms_170_20 crossref_primary_10_3390_diagnostics12061338 crossref_primary_10_1016_j_jcv_2023_105494 crossref_primary_10_1002_jmv_27285 crossref_primary_10_3390_v16040611 crossref_primary_10_1002_rmv_2342 crossref_primary_10_1016_j_jinf_2020_10_009 crossref_primary_10_15252_emmm_202115290 crossref_primary_10_1128_Spectrum_00855_21 crossref_primary_10_1016_j_bios_2022_114663 crossref_primary_10_7759_cureus_36962 crossref_primary_10_1038_s41579_022_00822_w crossref_primary_10_1073_pnas_2204624119 crossref_primary_10_1038_s41598_021_83959_7 crossref_primary_10_1177_00333549221114343 crossref_primary_10_3390_v13091773 crossref_primary_10_1128_JCM_00469_21 crossref_primary_10_3201_eid2702_204168 crossref_primary_10_3390_v15071555 crossref_primary_10_7326_M21_0422 crossref_primary_10_2807_1560_7917_ES_2021_26_14_2001506 crossref_primary_10_1093_cid_ciab436 crossref_primary_10_2478_prilozi_2024_0007 crossref_primary_10_1016_j_phoj_2021_10_001 crossref_primary_10_1128_JVI_00083_21 crossref_primary_10_1128_JCM_00083_21 crossref_primary_10_1186_s12889_023_15762_0 crossref_primary_10_1007_s10875_021_01045_z crossref_primary_10_1007_s40121_021_00413_x crossref_primary_10_1093_infdis_jiac048 crossref_primary_10_1007_s40291_021_00574_y crossref_primary_10_1016_S2666_5247_21_00056_2 crossref_primary_10_1128_spectrum_00503_22 crossref_primary_10_1128_spectrum_02264_21 crossref_primary_10_3390_jcm10184037 crossref_primary_10_2147_IDR_S333300 crossref_primary_10_3390_jcm10020328 crossref_primary_10_1016_j_jinf_2020_11_034 crossref_primary_10_1017_ice_2021_294 crossref_primary_10_3201_eid2711_211135 crossref_primary_10_1038_s41586_022_04702_4 crossref_primary_10_2139_ssrn_3987999 crossref_primary_10_1093_cid_ciab303 crossref_primary_10_1056_NEJMe2032179 crossref_primary_10_1103_PhysRevE_103_052303 crossref_primary_10_3390_diagnostics11122346 crossref_primary_10_1007_s10311_021_01186_y crossref_primary_10_1128_jcm_00501_22 crossref_primary_10_1016_j_ecoenv_2020_111438 crossref_primary_10_1515_cclm_2023_0846 crossref_primary_10_1038_s41390_021_01902_y crossref_primary_10_1128_JCM_02593_20 crossref_primary_10_1093_ofid_ofab310 crossref_primary_10_3390_ijerph18073377 crossref_primary_10_1093_infdis_jiad061 crossref_primary_10_3390_v13101923 crossref_primary_10_1001_jama_2022_21599 crossref_primary_10_3390_v13050818 crossref_primary_10_1016_j_ijid_2021_05_067 crossref_primary_10_1128_spectrum_03873_22 crossref_primary_10_1016_j_ebiom_2023_104669 crossref_primary_10_3390_v13112133 crossref_primary_10_1038_s43856_021_00034_y crossref_primary_10_1016_j_bjid_2022_102703 crossref_primary_10_1186_s12879_022_07240_6 crossref_primary_10_3389_fped_2021_642781 crossref_primary_10_1128_JCM_03077_20 crossref_primary_10_1093_infdis_jiab215 crossref_primary_10_1126_science_abi5273 crossref_primary_10_3389_fmed_2021_615099 crossref_primary_10_1111_his_14264 crossref_primary_10_1016_j_jviromet_2021_114102 crossref_primary_10_1093_ofid_ofab023 crossref_primary_10_1016_j_jcv_2021_104843 crossref_primary_10_1128_JCM_00896_21 crossref_primary_10_1016_j_virs_2022_01_008 crossref_primary_10_7326_M20_6519 crossref_primary_10_3390_v15051152 crossref_primary_10_1016_j_jviromet_2021_114182 crossref_primary_10_1371_journal_pone_0255691 crossref_primary_10_1371_journal_pone_0262656 crossref_primary_10_1002_aelm_202400908 |
| Cites_doi | 10.1093/clinchem/hvaa029 10.1093/cid/ciaa638 10.1038/s41591-020-0869-5 10.1016/j.jcv.2020.104346 10.3201/eid1002.030610 10.2807/1560-7917.ES.2020.25.16.2000421 10.1038/s41586-020-2196-x 10.1016/S1473-3099(20)30196-1 10.1038/s41586-020-2342-5 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2020 U.S. National Center for Infectious Diseases Published 2020. This article is a U.S. Government work and is in the public domain in the USA. |
| Copyright_xml | – notice: COPYRIGHT 2020 U.S. National Center for Infectious Diseases – notice: Published 2020. This article is a U.S. Government work and is in the public domain in the USA. |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7RV 7X7 7XB 8C1 8FI 8FJ 8FK ABUWG AEUYN AFKRA AZQEC BENPR CCPQU FYUFA GHDGH K9- K9. KB0 M0R M0S NAPCQ PHGZM PHGZT PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS 7X8 5PM DOA |
| DOI | 10.3201/eid2611.203219 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Nursing & Allied Health Database Health & Medical Collection ProQuest Central (purchase pre-March 2016) Public Health Database Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One Health Research Premium Collection Health Research Premium Collection (Alumni) Consumer Health Database ProQuest Health & Medical Complete (Alumni) Nursing & Allied Health Database (Alumni Edition) Consumer Health Database ProQuest Health & Medical Collection Nursing & Allied Health Premium ProQuest Central Premium ProQuest One Academic ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) ProQuest One Academic Middle East (New) ProQuest Central Essentials ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Family Health (Alumni Edition) ProQuest Central China ProQuest Central ProQuest One Sustainability Health Research Premium Collection Health and Medicine Complete (Alumni Edition) Health & Medical Research Collection ProQuest Central (New) ProQuest Public Health ProQuest Family Health ProQuest One Academic Eastern Edition ProQuest Nursing & Allied Health Source ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Hospital Collection (Alumni) Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition ProQuest Nursing & Allied Health Source (Alumni) ProQuest One Academic ProQuest One Academic (New) ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | ProQuest One Academic Middle East (New) MEDLINE MEDLINE - Academic |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: BENPR name: ProQuest Central url: https://www.proquest.com/central sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Public Health |
| DocumentTitleAlternate | SARS-CoV-2 Virus Culture and Subgenomic RNA |
| EISSN | 1080-6059 |
| EndPage | 2704 |
| ExternalDocumentID | oai_doaj_org_article_18629ec87d8c45cc8d3051ab28a63a98 PMC7588524 A642085550 32749957 10_3201_eid2611_203219 |
| Genre | Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
| GeographicLocations | Hong Kong Hong Kong, China China |
| GeographicLocations_xml | – name: Hong Kong – name: Hong Kong, China – name: China |
| GrantInformation_xml | – fundername: NIAID NIH HHS grantid: HHSN272201400006C |
| GroupedDBID | --- -~X .GJ 186 29G 2WC 36B 53G 5GY 5VS 6PF 7RV 7X7 85S 8C1 8FI 8FJ 9K5 A8Z AAWTL AAYOK AAYXX ABDBF ABUWG ACGFO ACUHS ADBBV ADRAZ AEGXH AENEX AETEA AEUYN AFKRA AIAGR ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS AZQEC B0M BAWUL BCNDV BENPR C1A CCPQU CITATION DIK DO4 E3Z EAD EAP EAS EBC EBD EBS EDH EJD EMB EMH EMI EMK EMOBN EPL EPT ESX F5P FRP FYUFA GROUPED_DOAJ GX1 HMCUK HYE IAO IBB IEA IEP IHR IHW IMI INH INR IOF IOV IPO ISR ITC K9- KQ8 M0R M48 NAPCQ NEJ O5R O5S OK1 OVT P2P PGMZT PHGZM PHGZT PV9 Q.E QF4 QM9 QN7 QO4 Q~Q RGD RGQ RNS RPM RXW RZL SV3 TAE TR2 TUS TWZ UKHRP UQL W2D WH7 WOQ WOW XJT XSB YRT YV5 YXF ZBA ZGI ~8M 0ZS CGR CUY CVF ECM EIF NPM PMFND 3V. 7XB 8FK K9. PJZUB PKEHL PPXIY PQEST PQQKQ PQUKI PRINS 7X8 5PM PUEGO |
| ID | FETCH-LOGICAL-c688t-c43267fbe1c18c1d3c9bd2ce9a3f41399ae09b458bb9bdc48b629fd77887fd063 |
| IEDL.DBID | M48 |
| ISSN | 1080-6040 1080-6059 |
| IngestDate | Wed Aug 27 01:30:04 EDT 2025 Thu Aug 21 14:11:15 EDT 2025 Thu Jul 10 22:34:58 EDT 2025 Fri Jul 25 04:53:19 EDT 2025 Tue Jun 17 21:59:26 EDT 2025 Thu Jun 12 23:57:49 EDT 2025 Tue Jun 10 21:02:53 EDT 2025 Fri Jun 27 05:15:17 EDT 2025 Fri Jun 27 05:15:10 EDT 2025 Thu May 22 21:23:30 EDT 2025 Thu Apr 03 07:04:37 EDT 2025 Tue Jul 01 03:29:59 EDT 2025 Thu Apr 24 23:07:01 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 11 |
| Keywords | viruses Hong Kong coronavirus zoonoses respiratory infections subgenomic RNA virus culture COVID-19 SARS-CoV-2 severe acute respiratory syndrome coronavirus 2 China coronavirus disease viral RNA load infectivity mild disease |
| Language | English |
| License | This is a publication of the U.S. Government. This publication is in the public domain and is therefore without copyright. All text from this work may be reprinted freely. Use of these materials should be properly cited. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c688t-c43267fbe1c18c1d3c9bd2ce9a3f41399ae09b458bb9bdc48b629fd77887fd063 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.3201/eid2611.203219 |
| PMID | 32749957 |
| PQID | 2466919957 |
| PQPubID | 105375 |
| PageCount | 4 |
| ParticipantIDs | doaj_primary_oai_doaj_org_article_18629ec87d8c45cc8d3051ab28a63a98 pubmedcentral_primary_oai_pubmedcentral_nih_gov_7588524 proquest_miscellaneous_2430658653 proquest_journals_2466919957 gale_infotracmisc_A642085550 gale_infotracgeneralonefile_A642085550 gale_infotracacademiconefile_A642085550 gale_incontextgauss_ISR_A642085550 gale_incontextgauss_IOV_A642085550 gale_healthsolutions_A642085550 pubmed_primary_32749957 crossref_citationtrail_10_3201_eid2611_203219 crossref_primary_10_3201_eid2611_203219 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2020-11-00 20201101 2020-11-01 |
| PublicationDateYYYYMMDD | 2020-11-01 |
| PublicationDate_xml | – month: 11 year: 2020 text: 2020-11-00 |
| PublicationDecade | 2020 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: Atlanta |
| PublicationTitle | Emerging infectious diseases |
| PublicationTitleAlternate | Emerg Infect Dis |
| PublicationYear | 2020 |
| Publisher | U.S. National Center for Infectious Diseases Centers for Disease Control and Prevention |
| Publisher_xml | – name: U.S. National Center for Infectious Diseases – name: Centers for Disease Control and Prevention |
| References | key-10.3201/eid2611.203219-202010181355-R10 To (key-10.3201/eid2611.203219-202010181355-R1) 2020; 20 He (key-10.3201/eid2611.203219-202010181355-R4) 2020; 26 Chan (key-10.3201/eid2611.203219-202010181355-R3) 2004; 10 Sia (key-10.3201/eid2611.203219-202010181355-R5) 2020; 583 Bullard (key-10.3201/eid2611.203219-202010181355-R9) 2020 Wölfel (key-10.3201/eid2611.203219-202010181355-R8) 2020; 581 Perera (key-10.3201/eid2611.203219-202010181355-R7) 2020; 25 Chu (key-10.3201/eid2611.203219-202010181355-R6) 2020; 66 Xiao (key-10.3201/eid2611.203219-202010181355-R2) 2020; 127 |
| References_xml | – ident: key-10.3201/eid2611.203219-202010181355-R10 – volume: 66 start-page: 549 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R6 article-title: Molecular diagnosis of a novel coronavirus (2019-nCoV) causing an outbreak of pneumonia. publication-title: Clin Chem doi: 10.1093/clinchem/hvaa029 – start-page: ciaa638 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R9 article-title: Predicting infectious SARS-CoV-2 from diagnostic samples. publication-title: Clin Infect Dis doi: 10.1093/cid/ciaa638 – volume: 26 start-page: 672 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R4 article-title: Temporal dynamics in viral shedding and transmissibility of COVID-19. publication-title: Nat Med doi: 10.1038/s41591-020-0869-5 – volume: 127 start-page: 104346 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R2 article-title: Dynamic profile of RT-PCR findings from 301 COVID-19 patients in Wuhan, China: A descriptive study. publication-title: J Clin Virol doi: 10.1016/j.jcv.2020.104346 – volume: 10 start-page: 294 year: 2004 ident: key-10.3201/eid2611.203219-202010181355-R3 article-title: Detection of SARS coronavirus in patients with suspected SARS. publication-title: Emerg Infect Dis doi: 10.3201/eid1002.030610 – volume: 25 start-page: 2000421 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R7 article-title: Serological assays for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), March 2020. publication-title: Euro Surveill doi: 10.2807/1560-7917.ES.2020.25.16.2000421 – volume: 581 start-page: 465 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R8 article-title: Virological assessment of hospitalized patients with COVID-2019. publication-title: Nature doi: 10.1038/s41586-020-2196-x – volume: 20 start-page: 565 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R1 article-title: Temporal profiles of viral load in posterior oropharyngeal saliva samples and serum antibody responses during infection by SARS-CoV-2: an observational cohort study. publication-title: Lancet Infect Dis doi: 10.1016/S1473-3099(20)30196-1 – volume: 583 start-page: 834 year: 2020 ident: key-10.3201/eid2611.203219-202010181355-R5 article-title: Pathogenesis and transmission of SARS-CoV-2 in golden hamsters. publication-title: Nature doi: 10.1038/s41586-020-2342-5 |
| SSID | ssj0012019 |
| Score | 2.6566558 |
| Snippet | We investigated 68 respiratory specimens from 35 coronavirus disease patients in Hong Kong, of whom 32 had mild disease. We found that severe acute respiratory... |
| SourceID | doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | 2701 |
| SubjectTerms | Adult Aged Betacoronavirus - genetics coronavirus coronavirus disease Coronavirus Infections - virology Coronaviruses COVID-19 Disease transmission Dispatch Expedited Female Genomes Hong Kong Humans Illnesses Male Middle Aged Molecular diagnostic techniques Pandemics Patients Pneumonia, Viral - virology Polymerase chain reaction Respiratory System - virology Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Viral - analysis SARS-CoV-2 SARS-CoV-2 Virus Culture and Subgenomic RNA for Respiratory Specimens from Patients with Mild Coronavirus Disease Severe acute respiratory syndrome coronavirus 2 Severity of Illness Index Virus research viruses |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV3db9MwELfQnpAQ4pvAAIMQe4rWJrZjP5bCNJAYqGXV3ix_ZVTaUlha_n7u7DQ0AgQPvMY_R5Hvch_23c-EvDS2LkB3ClRen7N6pHJIwUY5nrAJH9OiWG1xIo5P2fszfrZz1RfWhCV64LRwh2MIuVVwsvLSMe6c9KChY2MLaURpVGzzBZ-3Taa68wNwayqV1kNyBHqa6BpLeHwYlh6yBkwNywL5dXbcUWTt_9U27zinYeHkjic6ukVudiEknaRPv02uheYOuZH232hqK7pLvs0ns3k-XS3ygi6WV5uWJvLMQE3jKZgLJGe9hAmzkwmFwJXOfp6503gp_SUkuBS7T-inxL3aUty0pR-WF55OkfjAfI8vfpPOeO6R06O3n6fHeXe9Qu6ElOvcMQjdqtqGsRtLN_alU9YXLihT1uDalDJhpCzj0loYcExakETtK6w_rD2ENvfJXrNqwkNChWeWi9KqEYd8mztVgZmAWMQ5iCgklxnJt6usXcc9jldgXGjIQVAqupOKTlLJyEGP_5pYN_6IfI1C61HIlh0fgA7pTof033QoI89Q5Dq1nvb_vJ4ILD7gkMRl5EVEIGNGgyU552bTtvrdx8U_gOazAeigA9UrWAJnujYIWEhk4hogXw2Q54mH_HfA_QEQDIQbDm81WXcGqtUFE0Jhe36Vkef9MM7EorsmrDaIKTFAFbzMyIOk-P06l0XF0uxq8EsMBDEcaZZfIn05ZKiSF-zR_5DcY3K9wA2Q2By6T_bWV5vwBKLEtX0aDcIP9S9e4Q priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fb9MwELZgvCAhxO8VBhiE2FO0xkkc-wmVwjSQGKhlVd-s2E5KpS3Zmpa_nzvbzRrxQ7zGX6LIdz7fZ58_E_Km0BUD32HovDZKq6GMgIINI9xh49bRIldtccpPztLP82weFtzaUFa5jYkuUNvG4Br5EUs5l3ieOH93eRXhrVG4uxqu0LhJbqF0GXp1Pu8IVwyTm_QF9kCRwFu9aGMCj4_KpQXugAQxYaiyszMpOe3-3yP0zhTVL5_cmY-O75G7IZGkI2_5--RGWT8gd_wqHPWHix6Sq-loMo3GzSxidLZcbVrqJTRLWtSWQtBAidYLeGFyOqKQvtLJ9c47dVfTXwDNpXgGhX7zCqwtxaVb-mV5bukY5Q-Kn-7DH_xOzyNydvzx-_gkCpcsRIYLsY5MCglcXukyNrEwsU2M1JaZUhZJBROclEU5lDrNhNbQYFKhOZOVzbEKsbKQ4Dwme3VTl_uEcpvqjCdaDjNg3ZmROQQLyEiMgbxCZGJAom0vKxMUyPEijHMFTAStooJVlLfKgBx2-EuvvfFX5Hs0WodCzWz3oFktVBiCKgbyJksjcitMmhkjLMS6uNBMFDwpJPzeSzS58gdQu5GvRhxLEDKgcgPy2iFQN6PGwpxFsWlb9enr7D9A00kPdBhAVQNdYIpwGAI6EvW4esi3PeTCq5H_CXjQA0KYMP3mrSerEKZadT2oBuRV14xvYuldXTYbxCSYpvIsGZAn3vG7fk5Ynvq3896Q6Bmi31IvfzgRc-CpImPp03__1jNym-EChzv8eUD21qtN-RyywLV-4Yb6L63WVrc priority: 102 providerName: ProQuest |
| Title | SARS-CoV-2 Virus Culture and Subgenomic RNA for Respiratory Specimens from Patients with Mild Coronavirus Disease |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/32749957 https://www.proquest.com/docview/2466919957 https://www.proquest.com/docview/2430658653 https://pubmed.ncbi.nlm.nih.gov/PMC7588524 https://doaj.org/article/18629ec87d8c45cc8d3051ab28a63a98 |
| Volume | 26 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3fb9MwELZge0FCiN8ERjEIsaewxnEc-wGhtls1kFamllZ9i2I7KZW6lPUHgv-euzhtF1GkvazS_Lmq7PP5PvvuMyHvU50zsB2Gxmt9njeVDxSs6eMNm7AlLSqzLXrifMi_jqPxLv-pGsDlXmqH70kNF7OPv6__fIYF_wkZJ-xfJ9nUAg9AshcyVAA9hF0pxtcMLvjuRgGAyiXbA10Cy3UCjnv61zaoUsf_X299Y7uqp1Le2Ju6D8mDKqikLWcFj8idrHhM7rsTOeoKjZ6Q60GrP_A785HP6Gi6WC-pk9PMaFpYCg4E5VqvoEO_16IQytL-7haels_UXwHlpViPQi-dGuuS4jEuvZjOLO2gFEL6q_ziU3fr85QMu2ffO-d-9eCCb4SUK99wCObiXGeBCaQJbGiUtsxkKg1z2OyUSrOm0jySWkOD4VILpnIbY0ZibiHYeUYOinmRvSBUWK4jEWrVjICBR0bF4DggOjEGYgwZSY_4m1FOTKVGjo9izBJgJTgrSTUriZsVjxxv8T-dDsd_kW2ctC0K9bPLf8wXk6RajkkARE5lRsZWGh4ZIy34vSDVTKYiTBX8vDc45YkrRt16gaQlMB0hAlrnkXclAjU0CkzSmaTr5TL58m10C9CgXwMdV6B8DkNg0qowAgYStblqyA815MQpk-8DHtWA4DJMvXljyclmxSWMC6GwYD_2yNttM_bENLwim68RE2LIKqLQI8-d4W_HOWQxd73j2pKoTUS9pZj-KAXNgbPKiPGXtxnSV-QewyOPshz0iBysFuvsNcSFK90gd-NxDH9lJ2iQw1b7tN2Fz_ZZ77LfKM9aGqVL-AvWImD9 |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV3db9MwELfGeAAJIT5HYTCDgD1Fa5wv-wGh0jG1bCuo3aq9mcR2SqWRdE0L4p_ib-QuTrNGfIiXvcbnKPGd78O--x0hL-MkZSA7DIVXO37aFg6EYG0Hb9hCXYZFZbbFIOyd-h_OgrMN8nNVC4NplSudWCpqnSs8I99jfhgKrCeO3s4uHOwahberqxYaViwOzY_vELIVb_r7wN9XjB28P-n2nKqrgKNCzheO8sFjidLEuMrlytWeEolmyojYS0GjCxGbtkj8gCcJDCifJyETqY4w7S7VYNHhvdfIdd-Dv8PK9G6dUuKCMRU2oR9CMtgdFiTSg8d7ZqohVsGA1GOI6rNmBMteAb9bhDWT2EzXXLN_B3fI7cpxpR0raXfJhsnukVv21I_aYqb75GLUGY6cbj52GB1P58uCWshOQ-NMU1BSCAn7FSYMBx0K7jIdXt7009HMKOw1UFCseaGfLOJrQfGomB5PzzXtItxC_K188b69WXpATq9k-R-SzSzPzCNCQ-0nQegloh1AlB8oEYFyAg9IKfBjeMBbxFmtslQV4jk23jiXEPkgV2TFFWm50iK7Nf3MYn38lfIdMq2mQozu8kE-n8hqy0sXgkVhFI80V36gFNegW904YTwOvVjA5-0gy6UteK01jeyEmPIQQOjYIi9KCsTpyDARaBIvi0L2P47_g2g0bBDtVkRpDkug4qr4AhYS8b8alK8blBOLfv4nwu0GIagl1RxeSbKs1GIhLzdxizyvh3EmpvplJl8ijYducRh4LbJlBb9eZ49Fvp0dNbZEgxHNkWz6pQRNh7iYB8x__O_P2iE3eifHR_KoPzh8Qm4yPFwpC0-3yeZivjRPwQNdJM_KbU_J56vWM78AcIiUjg |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLbGkBASQlxHYTCDgD1FbZyb_YBQaak2BmVqWbU3k9hOqTSSrmlB_DV-HefEadaIi3jZa3wSRT53-5zvEPI8TlIGssNQeLXjpx3hQArWcfCGLdRlWlRWWwzDgxP_3WlwukV-rnthsKxybRNLQ61zhWfkbeaHocB-4qidVmURx_3B6_m5gxOk8KZ1PU7DisiR-fEd0rfi1WEfeP2CscHbT70Dp5ow4KiQ86WjfIheojQxrnK5crWnRKKZMiL2UrDuQsSmIxI_4EkCC8rnSchEqiMswUs1eHf47hVyNfIijjrGe3V5iQuOVdjifkjPQFMsYKQHj9tmpiFvweTUY4jws-EQy7kBv3uHDffYLN3c8IWDW-RmFcTSrpW622TLZHfIDXsCSG1j011yPu6Oxk4vnziMTmaLVUEtfKehcaYpGCyEh_0KL4yGXQqhMx1d3PrT8dwonDtQUOx_occW_bWgeGxMP8zONO0h9EL8rfxw394y3SMnl7L998l2lmfmAaGh9pMg9BLRCSDjD5SIwFBBNKQUxDQ84C3irHdZqgr9HIdwnEnIgpArsuKKtFxpkf2afm5xP_5K-QaZVlMhXnf5IF9MZaX-0oXEURjFI82VHyjFNdhZN04Yj0MvFvB7e8hyaZtfa6sjuyGWPwSQRrbIs5ICMTsylP5pvCoKefhx8h9E41GDaL8iSnPYAhVXjRiwkYgF1qB82aCcWiT0PxHuNgjBRKnm8lqSZWUiC3mh0C3ytF7GN7HsLzP5Cmk8DJHDwGuRHSv49T57LPLt21FDJRqMaK5ksy8lgDrkyDxg_sN__9YeuQYWRr4_HB49ItcZnrOUPai7ZHu5WJnHEIwukyel1lPy-bLNzC-iH5jE |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=SARS-CoV-2+Virus+Culture+and+Subgenomic+RNA+for+Respiratory+Specimens+from+Patients+with+Mild+Coronavirus+Disease&rft.jtitle=Emerging+infectious+diseases&rft.au=Perera%2C+Ranawaka+A.P.M&rft.au=Tso%2C+Eugene&rft.au=Tsang%2C+Owen+T.Y&rft.au=Tsang%2C+Dominic+N.C&rft.date=2020-11-01&rft.pub=U.S.+National+Center+for+Infectious+Diseases&rft.issn=1080-6040&rft.volume=26&rft.issue=11&rft.spage=2701&rft_id=info:doi/10.3201%2Feid2611.203219&rft.externalDBID=IOV&rft.externalDocID=A642085550 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1080-6040&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1080-6040&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1080-6040&client=summon |