Development of a recombinant replication-deficient rabies virus-based bivalent-vaccine against MERS-CoV and rabies virus and its humoral immunogenicity in mice

• Published in: PloS one Vol. 14; no. 10; p. e0223684
• Main Authors: Kato, Hirofumi, Takayama-Ito, Mutsuyo, Iizuka-Shiota, Itoe, Fukushi, Shuetsu, Posadas-Herrera, Guillermo, Horiya, Madoka, Satoh, Masaaki, Yoshikawa, Tomoki, Yamada, Souichi, Harada, Shizuko, Fujii, Hikaru, Shibamura, Miho, Inagaki, Takuya, Morimoto, Kinjiro, Saijo, Masayuki, Lim, Chang-Kweng
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.10.2019; Public Library of Science (PLoS)
• Subjects: Amino acids; Animal experimentation; Animals; Antibodies; Antibodies, Neutralizing - immunology; Antibodies, Viral - immunology; Biology and life sciences; Cell Line, Tumor; Chlorocebus aethiops; Coronaviridae; Coronavirus Infections - prevention & control; Coronaviruses; Deoxyribonucleic acid; DNA; Domains; Ebola virus; Female; Fluorescent antibody technique; Genes; Genetic aspects; Genetics; Genomes; Glycoproteins; HEK293 Cells; Humans; Immunofluorescence; Immunogenicity; Immunogenicity, Vaccine; Infectious diseases; Lyssavirus; Medicine and Health Sciences; Mice; Mice, Inbred BALB C; Mice, Inbred ICR; Middle East respiratory syndrome; Middle East Respiratory Syndrome Coronavirus - immunology; Neutralizing; Novels; Pathogenicity; Pathogens; Prevention; Proteins; Rabies; Rabies virus - genetics; Rabies virus - physiology; Replication; Respiratory diseases; Risk factors; RNA polymerase; Safety and security measures; Severe acute respiratory syndrome; Signs and symptoms; Spike glycoprotein; Spike Glycoprotein, Coronavirus - immunology; Vaccines; Vaccines, Synthetic - genetics; Vaccines, Synthetic - immunology; Vero Cells; Viral Vaccines - genetics; Viral Vaccines - immunology; Virology; Virus Replication; Viruses; Middle East; Saudi Arabia; Japan

Middle East respiratory syndrome-coronavirus (MERS-CoV) is an emerging virus that causes severe disease with fatal outcomes; however, there are currently no approved vaccines or specific treatments against MERS-CoV. Here, we developed a novel bivalent vaccine against MERS-CoV and rabies virus (RV) u...