Mouse methylation profiles for leukocyte cell types, and estimation of leukocyte fractions in inflamed gastrointestinal DNA samples

• Published in: PloS one Vol. 18; no. 10; p. e0290034
• Main Authors: Nishiyama, Kazuhiro, Nishinakamura, Hitomi, Takeshima, Hideyuki, Yuyu, Liu, Takeuchi, Chihiro, Hattori, Naoko, Takeda, Haruna, Yamashita, Satoshi, Wakabayashi, Mika, Sato, Kotomi, Obama, Kazutaka, Ushijima, Toshikazu
• Format: Journal Article
• Language: English
• Published: San Francisco Public Library of Science 05.10.2023; Public Library of Science (PLoS)
• Subjects: Algorithms; Analysis; Biology and life sciences; Biomarkers; CD4 antigen; CD45 antigen; CD8 antigen; Cluster analysis; Clustering; Colitis; Colon; Contamination; Coronary vessels; CpG islands; Dendritic cells; Deoxyribonucleic acid; Dextran; Dextran sulfate; Dextrans; DNA; DNA methylation; Enhancers; Evaluation; Flow cytometry; Gene expression; Genes; Genetic testing; Genomes; Genomics; Inflammation; Leukocytes; Lymphocytes; Lymphocytes B; Lymphocytes T; Medicine and Health Sciences; Methylation; Neutrophils; Physical Sciences; Research and Analysis Methods; RNA; Sodium; Software; Spleen; Sulfates; Tissue analysis; Japan

Precise analysis of tissue DNA and RNA samples is often hampered by contaminating non-target cells whose amounts are highly variable. DNA methylation profiles are specific to cell types, and can be utilized for assessment of the fraction of such contaminating non-target cells. Here, we aimed 1) to i...