Comparative evaluation of various DNA extraction methods and analysis of DNA degradation levels in commercially marketed Chestnut rose juices and beverages

• Published in: BMC biotechnology Vol. 25; no. 1; p. 9
• Main Authors: Ren, Yongchao, Ma, Yunlong, Li, Yanqi, Song, Yun, Zhao, WeiWei, Huang, Xuncai, Yu, Danmin, Li, Jian, Xu, Zuogang, Zhao, Wenjun
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 21.01.2025; BioMed Central; BMC
• Subjects: Beverages; Beverages - analysis; Castanea; Chemical properties; Chestnut; Chromatography; Composition effects; Consumers; Contamination; Decomposition (Chemistry); Degradation; Deoxyribonucleic acid; DNA; DNA degradation extent; DNA extraction procedures; DNA, Plant - analysis; DNA, Plant - chemistry; DNA, Plant - genetics; DNA, Plant - isolation & purification; Electrophoresis; Extraction (Chemistry); food chain; Food chains; Food composition; Food contamination; Food inspection; Food processing; Food products; Food quality; Food research; Food safety; Food selection; Food supply; Fourier transforms; Fraud; Fruit and Vegetable Juices - analysis; Fruit juices; gel electrophoresis; Gel electrophoresis technique; genes; humans; Juices; labor; Mass spectrometry; Measurement; Methods; NMR; Nuclear magnetic resonance; Polymerase chain reaction; Polyphenols; Processed foods; quantitative polymerase chain reaction; Raw materials; Real time; Real-time PCR technology; Real-Time Polymerase Chain Reaction; Rosa - chemistry; Rosa - genetics; Rosaceae; Scientific imaging; species identification; spectrophotometers; Spectrophotometric NanoDrop approach; Spectrophotometry; traceability; China; Beijing China; Chestnut rose juices and beverages; Spectrophotometric NanoDrop approach; Gel electrophoresis technique; DNA extraction procedures; Food safety; Real-time PCR technology; DNA degradation extent
• ISSN: 1472-6750; 1472-6750
• DOI: 10.1186/s12896-024-00933-7

Food safety is a significant global study subject that is strongly intertwined with human life and well-being. The utilization of DNA-based methods for species identification is a valuable instrument in the field of food inspection and regulation. It is particularly significant for traceability purp...