The amplicon-plus system for high-level expression of transgenes in plants

• Published in: Nature biotechnology Vol. 20; no. 6; pp. 622 - 625
• Main Authors: Mallory, Allison C., Parks, Graham, Endres, Matthew W., Baulcombe, David, Bowman, Lewis H., Pruss, Gail J., Vance, Vicki B.
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.06.2002; Nature; Nature Publishing Group
• Subjects: Agriculture; Arabidopsis thaliana; beta-glucuronidase; Bioinformatics; Biological and medical sciences; Biomedical and Life Sciences; Biomedical Engineering/Biotechnology; Biomedicine; Biotechnology; Cysteine Endopeptidases; Cysteine Endopeptidases - genetics; Cysteine Endopeptidases - immunology; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Regulation, Plant; Genetic Engineering; Genetic Engineering - methods; Genetic technics; genetics; Glucuronidase; Glucuronidase - genetics; immunology; Leaves; Life Sciences; methods; Methods. Procedures. Technologies; Modification of gene expression level; Nicotiana - genetics; Nicotiana - immunology; Nicotiana tabacum; Plant Leaves; Plant Leaves - genetics; Plant Leaves - immunology; Plants; Plants, Genetically Modified; Plants, Genetically Modified - genetics; Plants, Genetically Modified - immunology; Potato virus X; Potatoes; proteinases; RNA Interference; RNA Interference - immunology; technical-report; Tobacco; Tobacco etch virus; Transgenes; Transgenes - genetics; Transgenes - immunology; Transgenic animals and transgenic plants; Transgenic plants; Viral diseases; Viral Proteins; Viral Proteins - genetics; Viral Proteins - immunology; β-Glucuronidase; Enzyme; Gene coexpression; Potexvirus; Suppression; Gene expression; Tobacco etch virus; Virus; Gene silencing; Reporter gene; Potato virus X; Glycosidases; Hydrolases; Transgenic plant; Potyvirus; O-Glycosidases; Vector; Potyviridae

Many biotechnological applications require high-level expression of transgenes in plants. One strategy to achieve this goal was the production of potato virus X (PVX) “amplicon” lines: transgenic lines that encode a replicating RNA virus vector carrying a gene of interest 1 . The idea was that trans...