Enteral supplement enriched with glutamine, fiber, and oligosaccharide attenuates experimental colitis in mice

• Published in: Nutrition (Burbank, Los Angeles County, Calif.) Vol. 29; no. 3; pp. 549 - 555
• Main Authors: Joo, Erina, Yamane, Shunsuke, Hamasaki, Akihiro, Harada, Norio, Matsunaga, Tetsuro, Muraoka, Atsushi, Suzuki, Kazuyo, Nasteska, Daniela, Fukushima, Toru, Hayashi, Tatsuya, Tsuji, Hidemi, Shide, Kenichiro, Tsuda, Kinsuke, Inagaki, Nobuya
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.03.2013; Elsevier; Elsevier Limited
• Subjects: acute course; administration & dosage; analysis; Animals; Biological and medical sciences; Body weight; chemically induced; chemistry; chronic diseases; colitis; Colitis, Ulcerative; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - pathology; Colitis, Ulcerative - therapy; Colon; Colon - chemistry; Cytokines; Cytokines - genetics; Dextran Sulfate; Dextran sulfate sodium; Dietary fiber; Dietary Fiber - administration & dosage; Dietary Supplements; Disease Models, Animal; Drinking water; enteral feeding; Enteral Nutrition; Feeding. Feeding behavior; Fundamental and applied biological sciences. Psychology; Gastroenterology and Hepatology; Gastroenterology. Liver. Pancreas. Abdomen; gene expression; genetics; GFO; Glucagon-like peptide; Glucose; glutamine; Glutamine - administration & dosage; inflammation; Inflammatory bowel disease; Interleukin-1beta; Interleukin-1beta - genetics; Interleukin-1β; Intestinal Mucosa; Intestinal Mucosa - chemistry; Intestinal Mucosa - pathology; Japan; Male; Medical sciences; messenger RNA; Mice; Mice, Inbred C57BL; mucosa; Nutrition; Oligosaccharides; Oligosaccharides - administration & dosage; Other diseases. Semiology; pathology; Polymerase chain reaction; Receiving waters; RNA, Messenger; RNA, Messenger - analysis; Rodents; Sodium; sodium sulfate; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Studies; Sulfates; therapy; Ulcerative colitis; Vertebrates: anatomy and physiology, studies on body, several organs or systems; weight loss; Japan; GFO; Interleukin-1β; Glucagon-like peptide; Dextran sulfate sodium; Ulcerative colitis; Oligosaccharide; Cytokine; Rodentia; Interleukin 1β; Sulfates; Inflammatory disease; Vertebrata; Mammalia; Sodium; Mouse; Aminoacid; Animal; Digestive diseases; Intestinal disease; Colitis; Glutamine
• ISSN: 0899-9007; 1873-1244; 1873-1244
• DOI: 10.1016/j.nut.2012.09.007

Ulcerative colitis is a chronic recurrent disease characterized by acute inflammation of the colonic mucosa. In Japan, a dietary supplementation product enriched with glutamine, dietary fiber, and oligosaccharide (GFO) is widely applied for enteral nutrition support. These three components have been suggested to improve intestinal health. In this study, we investigated whether GFO has suppressive effects on mucosal damage in ulcerative colitis in an experimental mouse model. C57BL/6 mice received 2.5% dextran sulfate sodium in drinking water for 5 d to induce colitis. Then, they were given 0.25 mL of GFO or a 20% glucose solution twice daily for 10 d. Another set of mice receiving unaltered drinking water was used as the normal control group. The body weight loss and disease activity index were significantly lower in the GFO-treated mice compared with the glucose-treated mice (P < 0.05). The decrease in colon length induced by dextran sulfate sodium was significantly alleviated in GFO-treated mice compared with glucose-treated mice (P < 0.01). In addition, the histologic findings showed that intestinal inflammation was significantly attenuated in mice treated with GFO. Furthermore, treatment with GFO significantly inhibited the dextran sulfate sodium–induced increase in the mRNA expression of interleukin-1β. These results suggest that GFO has potential therapeutic value as an adjunct therapy for ulcerative colitis.