EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes

• Published in: Leukemia Vol. 26; no. 9; pp. 1908 - 1975
• Main Authors: van Dongen, J J M, Lhermitte, L, Böttcher, S, Almeida, J, van der Velden, V H J, Flores-Montero, J, Rawstron, A, Asnafi, V, Lécrevisse, Q, Lucio, P, Mejstrikova, E, Szczepański, T, Kalina, T, de Tute, R, Brüggemann, M, Sedek, L, Cullen, M, Langerak, A W, Mendonça, A, Macintyre, E, Martin-Ayuso, M, Hrusak, O, Vidriales, M B, Orfao, A
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.09.2012; Nature Publishing Group; Springer Nature
• Subjects: 631/1647/1407/1492; 692/699/67/1990; Algorithms; Antibodies; Antibodies, Monoclonal; Biomarkers, Tumor - immunology; Cancer Research; Case-Control Studies; Cloning; Consortia; Critical Care Medicine; Diagnosis; Europe; Flow Cytometry - standards; Hematologic Neoplasms - diagnosis; Hematologic Neoplasms - immunology; Hematology; Human health and pathology; Humans; Immunology; Immunophenotyping - standards; Intensive; Internal Medicine; Leukemia; Leukocytes; Leukocytes - immunology; Leukocytes - pathology; Life Sciences; Lymphoma; Medicine; Medicine & Public Health; Oncology; Original; original-article; Pediatrics; Physiological aspects; Prognosis; Viral antibodies; Europe; United States; Netherlands; Spain; hematological malignancies; 8-color immunostaining; lymphoma; standardization; flow cytometry; EuroFlow; antibody panel

Most consensus leukemia & lymphoma antibody panels consist of lists of markers based on expert opinions, but they have not been validated. Here we present the validated EuroFlow 8-color antibody panels for immunophenotyping of hematological malignancies. The single-tube screening panels and mult...