Extracellular matrix production by adipose-derived stem cells: Implications for heart valve tissue engineering

• Published in: Biomaterials Vol. 32; no. 1; pp. 119 - 127
• Main Authors: Colazzo, Francesca, Sarathchandra, Padmini, Smolenski, Ryszard T., Chester, Adrian H., Tseng, Yuan-Tsan, Czernuszka, Jan T., Yacoub, Magdi H., Taylor, Patricia M.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.01.2011
• Subjects: adipose tissue; Adipose Tissue - cytology; Adipose-derived stem cells; Adult; Advanced Basic Science; Amino Acids; Amino Acids - metabolism; aorta; bone marrow; cattle; Cell Shape; Cell Shape - drug effects; chemistry; Collagen; Collagen - metabolism; Collagens; Cross-Linking Reagents; Cross-Linking Reagents - pharmacology; Crosslinking; Crosslinks; cytology; Dentistry; Desmosine; Desmosine - metabolism; drug effects; Elastin; Elastin - metabolism; enzymes; Extracellular matrix; Extracellular Matrix - drug effects; Extracellular Matrix - metabolism; fluorescent antibody technique; gene expression regulation; Gene Expression Regulation - drug effects; genes; Heart valve; Heart Valve Prosthesis; Heart valves; Human; Humans; liquid chromatography; mass spectrometry; Mesenchymal Stem Cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells - ultrastructure; metabolism; methods; Middle Aged; pharmacology; Phenotype; proline; Proline - metabolism; quantitative polymerase chain reaction; radiolabeling; Scaffolds; sowing; Stem cells; Stem Cells - cytology; Stem Cells - drug effects; Stem Cells - metabolism; Stem Cells - ultrastructure; Stress, Mechanical; Tissue engineering; Tissue Engineering - methods; tissue scaffolds; Tissue Scaffolds - chemistry; transmission electron microscopy; tritium; ultrastructure; Valves; Crosslinks; Extracellular matrix; Adipose-derived stem cells; Heart valve; Collagen; Elastin

A key challenge in tissue engineering a heart valve is to reproduce the major tissue structures responsible for native valve function. Here we evaluated human adipose-derived stem cells (ADSCs) as a source of cells for heart valve tissue engineering investigating their ability to synthesize and proc...