Matrix stiffness controls lymphatic vessel formation through regulation of a GATA2-dependent transcriptional program

• Published in: Nature communications Vol. 9; no. 1; pp. 1511 - 16
• Main Authors: Frye, Maike, Taddei, Andrea, Dierkes, Cathrin, Martinez-Corral, Ines, Fielden, Matthew, Ortsäter, Henrik, Kazenwadel, Jan, Calado, Dinis P., Ostergaard, Pia, Salminen, Marjo, He, Liqun, Harvey, Natasha L., Kiefer, Friedemann, Mäkinen, Taija
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 17.04.2018; Nature Publishing Group; Nature Portfolio
• Subjects: 13/1; 13/31; 13/51; 13/89; 14; 14/19; 14/3; 38; 38/1; 38/15; 38/39; 38/61; 45/88; 45/90; 631/136/2060/290; 631/80/79/2066; 631/80/84/750; 64/110; Animal models; Animals; Atomic force microscopy; Blood vessels; Cell adhesion & migration; Cell culture; Cell migration; Cell Movement - genetics; Embryos; Endothelial cells; Endothelial Cells - physiology; Extracellular matrix; Female; GATA2 Transcription Factor - genetics; GATA2 Transcription Factor - metabolism; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Developmental; Gene Knockdown Techniques; Gene regulation; Humanities and Social Sciences; Humans; In vivo methods and tests; Lymphangiogenesis - genetics; Lymphatic system; Lymphatic Vessels - cytology; Lymphatic Vessels - physiology; Male; Mice; Mice, Transgenic; Morphogenesis; multidisciplinary; Primary Cell Culture; RNA, Small Interfering - metabolism; Rodents; Science; Science (multidisciplinary); Stiffening; Stiffness; Substrates; Transcription; Vascular endothelial growth factor; Vascular Endothelial Growth Factor C - metabolism; Vascular Endothelial Growth Factor Receptor-3 - genetics; Vascular Endothelial Growth Factor Receptor-3 - metabolism; Vascular endothelial growth factor receptors

Tissue and vessel wall stiffening alters endothelial cell properties and contributes to vascular dysfunction. However, whether extracellular matrix (ECM) stiffness impacts vascular development is not known. Here we show that matrix stiffness controls lymphatic vascular morphogenesis. Atomic force mi...