Direct Drug Metabolism Monitoring in a Live Single Hepatic Cell by Video Mass Spectrometry

The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope. The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced...

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Bibliographic Details
Published inAnalytical Sciences Vol. 28; no. 3; p. 201
Main Authors DATE, Sachiko, MIZUNO, Hajime, TSUYAMA, Naohiro, HARADA, Takanori, MASUJIMA, Tsutomu
Format Journal Article
LanguageEnglish
Published Singapore The Japan Society for Analytical Chemistry 2012
Springer Nature Singapore
Japan Science and Technology Agency
Subjects
Analytical Chemistry
Antineoplastic Agents - metabolism
Cell Survival
Chemistry
Hep G2 Cells
Humans
Liver - cytology
Mass Spectrometry - economics
Mass Spectrometry - methods
Rapid Communications
Single-Cell Analysis - economics
Single-Cell Analysis - methods
Tamoxifen - metabolism
Time Factors
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Summary:The metabolism of anti-breast cancer drug, tamoxifen, in a single human hepatocellular carcinoma cell, HepG2, was directly monitored by a video-mass spectroscope. The cytoplasm, a vacuole or nucleus of the cell was directly sucked by a nano-spray tip under a video-microscope, and then was introduced into a mass spectrometer. Unchanged drug molecules were found in cytoplasm and a vacuole, but the metabolites were only found in the cytoplasm. This direct detection of drug metabolites in a live single cell is useful for speedy drug metabolism monitoring.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:0910-6340
1348-2246
DOI:10.2116/analsci.28.201