The effect of heating against Cryptosporidium oocysts

• Published in: Journal of Veterinary Medical Science Vol. 64; no. 3; pp. 199 - 200
• Main Authors: Fujino, T. (Kyorin Univ., Hachioji, Tokyo (Japan)), Matsui, T, Kobayashi, F, Haruki, K, Yoshino, Y, Kajima, J, Tsuji, M
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.03.2002; Japan Science and Technology Agency
• Subjects: Animals; chicken Cryptosporidium; CHICKENS; Cryptosporidiosis - veterinary; CRYPTOSPORIDIUM; Cryptosporidium - growth & development; Cryptosporidium muris; Cryptosporidium parvum; Feces - parasitology; HEAT; Heat treatment; Hot Temperature; INFECTION; Infectivity; infectivity of heated oocyst; Inoculation; Japan; Mice; Mice, Inbred ICR; Mice, SCID; Oocysts; Oocytes - physiology; Poultry; Poultry Diseases - parasitology; Protozoa; Specific Pathogen-Free Organisms; Japan
• DOI: 10.1292/jvms.64.199
• ISSN: 0916-7250

The effect of heat treatment was examined against oocysts of Cryptosporidium parvum, Cryptosporidium tnuris and chicken Cryptosporidium sp. isolated in Japan. The oocysts of these species were exposed at 50, 55, 60 and 70degC for 5, 15, 30 and 60 sec in water bath, respectively. To determine the infectivity of heated oocysts, the mice and chickens were inoculated with the treated oocysts and the oocyst output in the feces after inoculation was examined. In C. parvum and chicken Cryptosporidium sp., the oocysts were not detected from mice or chickens which were received oocysts heated at 55degC for 30 sec, 60degC for 15 sec and 70degC for 5 sec. In C. muris, the oocysts were not detected from mice which were received oocysts heated at 55 deg C for 15 sec, 60degC for 15 sec and 70degC for 5 sec. Consequently, it was clarified that the infectivity of Cryptosporidium oocysts to mice and chickens was lost by heating at 55degC for 30 sec, 60degC for 15 sec and 70degC for 5 sec.