Up-regulation of pro-inflammatory genes as adaptation to hypoxia in MCF-7 cells and in human mammary invasive carcinoma microenvironment

• Published in: Cancer science Vol. 101; no. 4; pp. 1014 - 1023
• Main Authors: Tafani, Marco, Russo, Andrea, Di Vito, Maura, Sale, Patrizio, Pellegrini, Laura, Schito, Luana, Gentileschi, Stefano, Bracaglia, Roberto, Marandino, Ferdinando, Garaci, Enrico, Russo, Matteo A
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.04.2010; Blackwell; John Wiley & Sons, Inc
• Subjects: Acute phase proteins; Adenocarcinoma; Advanced glycosylation end products; Aged; Antibodies; Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - immunology; Breast Neoplasms - metabolism; Cell activation; Cell Hypoxia - genetics; Cell Line, Tumor; Cell migration; Chemokines; CXCR4 protein; Cyclooxygenase-2; Cytokines; Enzymes; Estrogen receptors; Female; Fibroblast growth factor 2; Gene expression; Gene regulation; Genes; Genotype & phenotype; Glycosylation; Goats; Growth factors; Gynecology. Andrology. Obstetrics; Humans; Hypoxia; Hypoxia-Inducible Factor 1, alpha Subunit - metabolism; Hypoxia-inducible factor 1a; Inflammation; Inflammation - genetics; Invasiveness; Mammary gland; Mammary gland diseases; Medical sciences; Middle Aged; Neoplasm Invasiveness - genetics; NF-kappa B - antagonists & inhibitors; NF-kappa B - genetics; NF-kappa B - metabolism; NF-κB protein; Nitric-oxide synthase; Nuclear transport; Original; Pentraxins; Phenotypes; Proteins; Transcription; Transcription activation; Transformed cells; Tumor cells; Tumor microenvironment; Tumors; Up-Regulation; United States > US; Human; Oxygen; Breast disease; Breast cancer; Inflammation; Malignant tumor; Microenvironment; Mammary gland diseases; Breast carcinoma; Cancerology; Gene; Established cell line; Genetics; Hypoxia; Invasive cancer; Cancer; Adaptation

The role of tumor cells in synthesizing pro‐inflammatory molecules is still controversial. Here we report that hypoxic treatment of the MCF‐7 human mammary adenocarcinoma cell line induced activation of hypoxia‐inducible factor 1α (HIF‐1α) and nuclear factor‐kappa B (NF‐κB). Importantly, hypoxia reg...