molecular investigative approach to an outbreak of acute hemorrhagic conjunctivitis in Egypt, October 2010

• Published in: Virology journal Vol. 10; no. 1; p. 96
• Main Authors: Ayoub, Ehab A, Shafik, Caroline F, Gaynor, Anne M, Mohareb, Emad W, Amin, Magdy A, Yassin, Aymen S, El-Refaey, Samir, Genedy, Mohamed, Kandeel, Amr
• Format: Journal Article
• Language: English
• Published: England Springer-Verlag 25.03.2013; BioMed Central Ltd; BioMed Central
• Subjects: Adenoviruses; Adenoviruses, Human - genetics; Adenoviruses, Human - isolation & purification; biomedical research; Cell culture; conjunctivitis; Conjunctivitis, Acute Hemorrhagic - epidemiology; Conjunctivitis, Acute Hemorrhagic - virology; Coxsackievirus; Coxsackievirus infections; Development and progression; Disease Outbreaks; Egypt; Egypt - epidemiology; Enterovirus; Enterovirus - classification; Enterovirus - genetics; Enterovirus - isolation & purification; etiological agents; Female; Genomes; Genotype; Human adenovirus; Humans; Mastadenovirus; Microbiology; Molecular Sequence Data; Molecular Typing - methods; Mutation; patients; Phylogenetics; Phylogeny; Polymerase chain reaction; Prevention; Proteins; Risk factors; RNA polymerase; Viral infections; viruses; United States; China; Egypt; Singapore; Taiwan; Cairo Egypt; USA; Egypt, Arab Rep., Ash Sharqiyah, Port Said; China, People's Rep
• DOI: 10.1186/1743-422x-10-96
• ISSN: 1743-422X

BACKGROUND: During October 2010, Egypt reported an outbreak of acute hemorrhagic conjunctivitis (AHC). A total of 1831 cases were reported from three governorates; 1703 cases in El Daqahliya, 92 cases in Port Said, and 36 in Damietta. The purpose of this study was to identify and characterize the causative agent associated with this outbreak. METHODS: The U.S. Naval Medical Research Unit No.3 (NAMRU-3) was contacted by the Egyptian Ministry of Health and Population to perform diagnostic laboratory testing on eighteen conjunctival swabs from patients with conjunctivitis from El Daqahliya Governorate. Conjunctival swabs were tested by molecular methods for human adenovirus (HAdV) and enteroviruses (EV). Virus isolation was performed; the isolated virus was further characterized by molecular typing and phylogenetic analysis. RESULTS: The majority of the samples (17/18) were positive for enterovirus and all were negative for HAdV. Molecular typing and sequencing of the isolated virus revealed the presence of coxsackievirus A24 variant. Phylogenetic analysis based on the VP1 and 3C regions demonstrated that the Egyptian viruses belonged to Genotype IV and are closely related to coxsackievirus A24 variant, reported in a similar outbreak in China in August 2010. CONCLUSIONS: This study strongly suggests that coxsackievirus A24 variant was associated with the acute hemorrhagic conjunctivitis outbreak reported in Egypt in October 2010. There is a possibility that the same strain of CV-A24v was implicated in the AHC outbreaks in both China and Egypt in 2010.