In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus
Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional en...
Saved in:
Published in | Nature (London) Vol. 527; no. 7579; pp. 531 - 534 |
---|---|
Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
England
Nature Publishing Group
26.11.2015
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Abstract | Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional enzyme complex (TEC). By direct electron-counting cryoelectron microscopy and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the ten segmented dsRNAs in CPV are organized with ten TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. The TEC consists of two extensively interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV is converted to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An amino-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus. |
---|---|
AbstractList | Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional enzyme complex (TEC)1. By direct electron-counting cryoelectron microscopy and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the ten segmented dsRNAs in CPV are organized with ten TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. The TEC consists of two extensively interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV is converted to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An amino-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus. Viruses in the Reoviridae , like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented dsRNAs inside the viral capsid and carry out endogenous mRNA synthesis through a transcriptional enzyme complex (TEC). By direct electron-counting cryoEM and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the total 10 segmented dsRNAs in CPV are organized with 10 TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. TEC consists of two extensively-interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes significant conformational change when converted from q-CPV to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An N-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus. Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of segmented double-stranded RNAs (dsRNAs) inside the viral capsid and carry out endogenous messenger RNA synthesis through a transcriptional enzyme complex (TEC). By direct electron-counting cryoelectron microscopy and asymmetric reconstruction, we have determined the organization of the dsRNA genome inside quiescent CPV (q-CPV) and the in situ atomic structures of TEC within CPV in both quiescent and transcribing (t-CPV) states. We show that the ten segmented dsRNAs in CPV are organized with ten TECs in a specific, non-symmetric manner, with each dsRNA segment attached directly to a TEC. The TEC consists of two extensively interacting subunits: an RNA-dependent RNA polymerase (RdRP) and an NTPase VP4. We find that the bracelet domain of RdRP undergoes marked conformational change when q-CPV is converted to t-CPV, leading to formation of the RNA template entry channel and access to the polymerase active site. An amino-terminal helix from each of two subunits of the capsid shell protein (CSP) interacts with VP4 and RdRP. These findings establish the link between sensing of environmental cues by the external proteins and activation of endogenous RNA transcription by the TEC inside the virus. |
Audience | Academic |
Author | Yu, Xuekui Sun, Jingchen Chang, Winston Zhang, Xing Zhou, Z Hong Ding, Ke |
AuthorAffiliation | 3 Bioengineering, University of California, Los Angeles, CA 90095, USA 1 California Nanosystems Institute, Los Angeles, CA 90095, USA 2 Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA 4 Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China |
AuthorAffiliation_xml | – name: 1 California Nanosystems Institute, Los Angeles, CA 90095, USA – name: 4 Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China – name: 2 Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA – name: 3 Bioengineering, University of California, Los Angeles, CA 90095, USA |
Author_xml | – sequence: 1 givenname: Xing surname: Zhang fullname: Zhang, Xing organization: California Nanosystems Institute, Los Angeles, CA 90095, USA – sequence: 2 givenname: Ke surname: Ding fullname: Ding, Ke organization: Bioengineering, University of California, Los Angeles, CA 90095, USA – sequence: 3 givenname: Xuekui surname: Yu fullname: Yu, Xuekui organization: Department of Microbiology, Immunology and Molecular Genetics, University of California, Los Angeles, CA 90095, USA – sequence: 4 givenname: Winston surname: Chang fullname: Chang, Winston organization: California Nanosystems Institute, Los Angeles, CA 90095, USA – sequence: 5 givenname: Jingchen surname: Sun fullname: Sun, Jingchen organization: Subtropical Sericulture and Mulberry Resources Protection and Safety Engineering Research Center, Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou, Guangdong 510642, China – sequence: 6 givenname: Z Hong surname: Zhou fullname: Zhou, Z Hong organization: Bioengineering, University of California, Los Angeles, CA 90095, USA |
BackLink | https://www.ncbi.nlm.nih.gov/pubmed/26503045$$D View this record in MEDLINE/PubMed |
BookMark | eNp10u9r1DAYB_AgE3ebvvK9BH2jSGfStEnvzeA4_HEwJsyJ4JuQpE9uGW3SJe3Y_ntzOOednPRFoP3km_R5niN04IMHhF5SckIJaz54NU4RaC24eIJmtBK8qHgjDtCMkLIpSMP4ITpK6ZoQUlNRPUOHJa8JI1U9Qz9XHic3TjiNcTKboISDxeMV4ATrHvwILV6DDz1g5Vt8cb7AQ-jue4gqATahHzq4w84n12aB27QRty5O6Tl6alWX4MXDeoy-f_p4ufxSnH39vFouzgrDhRgL3WiuRWMJaNMoQ-dGMEtUSa2udD237VxpQYUurVXAbb65Yqyuq1JXFFrN2TE6_Z07TLqH1uQ7R9XJIbpexXsZlJO7X7y7kutwK2vS8LIWOeDtQ0AMNxOkUfYuGeg65SFMSVLBRNUQykimb_6h12GKPv_eRjWkYrnGf9VadSCdtyGfazahclExXjIq6jKrYo_Ktc6l7XKLrcuvd_zrPd4M7kZuo5M9KD8t9M7sTX23syGbEe7GtZpSkqtvF7v2_f_t4vLH8nyvNjGkFME-toQSuZlcuTW5Wb_a7uKj_TOq7BcFIeg_ |
CODEN | NATUAS |
CitedBy_id | crossref_primary_10_1038_s41586_019_1229_9 crossref_primary_10_1016_j_dci_2021_104244 crossref_primary_10_1016_j_sbi_2017_07_002 crossref_primary_10_1007_s12551_019_00572_9 crossref_primary_10_1128_mBio_01242_18 crossref_primary_10_7554_eLife_25940 crossref_primary_10_1007_s00438_019_01551_1 crossref_primary_10_1128_JVI_01964_18 crossref_primary_10_1007_s00705_017_3412_3 crossref_primary_10_1128_mbio_00156_22 crossref_primary_10_1038_s41594_019_0328_5 crossref_primary_10_1016_j_coviro_2018_08_001 crossref_primary_10_18632_oncotarget_12496 crossref_primary_10_1073_pnas_2203054119 crossref_primary_10_1038_s41467_019_10236_7 crossref_primary_10_1039_D3NA00393K crossref_primary_10_1126_sciadv_1501929 crossref_primary_10_1371_journal_ppat_1006242 crossref_primary_10_1261_rna_071100_119 crossref_primary_10_3390_v10090481 crossref_primary_10_1073_pnas_1915078117 crossref_primary_10_1017_S0033583521000068 crossref_primary_10_1007_s00705_021_05142_7 crossref_primary_10_1016_j_jsb_2017_10_007 crossref_primary_10_1016_j_virusres_2020_198261 crossref_primary_10_1371_journal_ppat_1012037 crossref_primary_10_1038_s41467_021_24455_4 crossref_primary_10_1111_tra_12768 crossref_primary_10_1371_journal_ppat_1011184 crossref_primary_10_1038_s41467_022_28114_0 crossref_primary_10_1128_spectrum_04938_22 crossref_primary_10_1371_journal_ppat_1005625 crossref_primary_10_1099_jgv_0_000463 crossref_primary_10_3390_v10050222 crossref_primary_10_1128_mbio_00499_24 crossref_primary_10_1042_BSR20170203 crossref_primary_10_1038_s42003_022_03793_z crossref_primary_10_1016_j_ijbiomac_2022_04_136 crossref_primary_10_1016_j_coviro_2021_09_008 crossref_primary_10_1128_mBio_02924_20 crossref_primary_10_3390_v15122404 crossref_primary_10_1111_mmi_14161 crossref_primary_10_1371_journal_ppat_1012140 crossref_primary_10_1073_pnas_2006165117 crossref_primary_10_1038_ncomms12524 crossref_primary_10_1038_nrmicro_2016_46 crossref_primary_10_1128_JVI_00968_18 crossref_primary_10_1016_j_jmb_2019_09_015 crossref_primary_10_1038_nature20589 crossref_primary_10_1042_BSR20170072 crossref_primary_10_1128_JVI_00774_18 crossref_primary_10_3390_v13020313 crossref_primary_10_1146_annurev_virology_092920_021307 crossref_primary_10_1128_JVI_01049_19 crossref_primary_10_1073_pnas_1905849116 crossref_primary_10_1016_j_str_2020_01_011 crossref_primary_10_1007_s41048_017_0038_7 crossref_primary_10_1016_j_sbi_2018_07_011 crossref_primary_10_1007_s11427_020_1752_1 crossref_primary_10_1371_journal_pntd_0011404 crossref_primary_10_1038_s41594_019_0320_0 crossref_primary_10_1128_JVI_02416_16 crossref_primary_10_1016_j_tibs_2020_05_002 crossref_primary_10_1016_j_str_2020_05_007 crossref_primary_10_1073_pnas_1904918116 crossref_primary_10_1073_pnas_1809304115 crossref_primary_10_1371_journal_ppat_1005523 crossref_primary_10_1128_JVI_01013_16 crossref_primary_10_1128_JVI_01542_20 crossref_primary_10_1038_ncomms13223 crossref_primary_10_1016_j_str_2023_09_008 crossref_primary_10_1016_j_virusres_2019_197822 crossref_primary_10_1038_nature19948 crossref_primary_10_1093_nar_gkad274 crossref_primary_10_1128_JVI_01774_18 |
Cites_doi | 10.1128/JVI.64.10.4988-4996.1990 10.1128/JVI.71.10.7220-7226.1997 10.1128/JVI.02360-10 10.1006/viro.2001.1258 10.1038/35010041 10.1074/jbc.M205964200 10.1006/viro.1993.1132 10.1016/j.jsb.2006.08.021 10.1016/S0092-8674(00)80758-8 10.1016/0092-8674(91)90324-R 10.1016/S0092-8674(02)01110-8 10.1529/biophysj.104.052811 10.1016/j.jmb.2012.10.011 10.7554/eLife.01299 10.1073/pnas.0914604107 10.1093/nar/1.6.809 10.1107/S0907444909052925 10.7554/eLife.07901 10.1073/pnas.0711623105 10.1002/jcc.20084 10.1016/S0092-8674(00)81773-0 10.1038/nature04487 10.1126/science.1127981 10.1074/jbc.M308637200 10.1016/j.str.2008.09.006 10.1016/j.jsb.2012.09.006 10.1016/j.coviro.2014.08.008 10.1016/S1047-8477(03)00069-8 10.1016/j.jsb.2013.07.005 10.1107/S0907444904019158 10.1128/JVI.78.15.8059-8067.2004 10.1038/nature06893 10.1128/JVI.78.10.5528-5530.2004 10.1038/26694 10.1016/j.jsb.2005.03.009 10.1073/pnas.1200206109 10.1038/nsmb1225 10.1007/s00705-003-0103-z 10.1128/JVI.71.12.9618-9626.1997 10.1038/nsb1009 10.1016/j.str.2011.03.003 10.1016/j.sbi.2009.10.015 10.1016/j.jsb.2006.09.002 |
ContentType | Journal Article |
Copyright | COPYRIGHT 2015 Nature Publishing Group Copyright Nature Publishing Group Nov 26, 2015 |
Copyright_xml | – notice: COPYRIGHT 2015 Nature Publishing Group – notice: Copyright Nature Publishing Group Nov 26, 2015 |
DBID | CGR CUY CVF ECM EIF NPM AAYXX CITATION ATWCN 3V. 7QG 7QL 7QP 7QR 7RV 7SN 7SS 7ST 7T5 7TG 7TK 7TM 7TO 7U9 7X2 7X7 7XB 88A 88E 88G 88I 8AF 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK 8G5 ABJCF ABUWG AFKRA ARAPS ATCPS AZQEC BBNVY BEC BENPR BGLVJ BHPHI BKSAR C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ GUQSH H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M2M M2O M2P M7N M7P M7S MBDVC NAPCQ P5Z P62 P64 PATMY PCBAR PDBOC PQEST PQQKQ PQUKI PSYQQ PTHSS PYCSY Q9U R05 RC3 S0X SOI 7X8 5PM |
DOI | 10.1038/nature15767 |
DatabaseName | Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Gale In Context: Middle School ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Calcium & Calcified Tissue Abstracts Chemoreception Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Environment Abstracts Immunology Abstracts Meteorological & Geoastrophysical Abstracts Neurosciences Abstracts Nucleic Acids Abstracts Oncogenes and Growth Factors Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection ProQuest Central (purchase pre-March 2016) Biology Database (Alumni Edition) Medical Database (Alumni Edition) Psychology Database (Alumni) Science Database (Alumni Edition) STEM Database ProQuest Pharma Collection Public Health Database Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Research Library (Alumni Edition) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest Central Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection eLibrary AUTh Library subscriptions: ProQuest Central Technology Collection Natural Science Collection Earth, Atmospheric & Aquatic Science Collection Environmental Sciences and Pollution Management ProQuest One Community College ProQuest Materials Science Collection ProQuest Central Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student Research Library Prep AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection ProQuest Biological Science Collection Agriculture Science Database Health & Medical Collection (Alumni Edition) PML(ProQuest Medical Library) Psychology Database Research Library Science Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database Engineering Database Research Library (Corporate) Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Earth, Atmospheric & Aquatic Science Database Materials Science Collection ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest One Psychology Engineering Collection Environmental Science Collection ProQuest Central Basic University of Michigan Genetics Abstracts SIRS Editorial Environment Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Agricultural Science Database ProQuest One Psychology Research Library Prep ProQuest Central Student Oncogenes and Growth Factors Abstracts ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts elibrary ProQuest AP Science SciTech Premium Collection Environmental Sciences and Pollution Management Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Biological Science Collection Chemoreception Abstracts ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Science Journals (Alumni Edition) ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Earth, Atmospheric & Aquatic Science Database Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts Neurosciences Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Calcium & Calcified Tissue Abstracts Meteorological & Geoastrophysical Abstracts - Academic University of Michigan Technology Collection Technology Research Database SIRS Editorial Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College Research Library (Alumni Edition) ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Biology Journals (Alumni Edition) ProQuest Central Earth, Atmospheric & Aquatic Science Collection Genetics Abstracts ProQuest Engineering Collection Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Research Library ProQuest Materials Science Collection ProQuest Public Health ProQuest Central Basic ProQuest Science Journals ProQuest Nursing & Allied Health Source ProQuest Psychology Journals (Alumni) ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library ProQuest Psychology Journals Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts Environment Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
DatabaseTitleList | Agricultural Science Database MEDLINE MEDLINE - Academic |
Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
DeliveryMethod | fulltext_linktorsrc |
Discipline | Sciences (General) Physics |
EISSN | 1476-4687 |
EndPage | 534 |
ExternalDocumentID | 3881023111 A436231752 10_1038_nature15767 26503045 |
Genre | Research Support, U.S. Gov't, Non-P.H.S Research Support, Non-U.S. Gov't Journal Article Research Support, N.I.H., Extramural |
GeographicLocations | California |
GeographicLocations_xml | – name: California |
GrantInformation_xml | – fundername: NIH HHS grantid: S10 OD018111 – fundername: NIAID NIH HHS grantid: R01 AI094386 – fundername: NIGMS NIH HHS grantid: GM071940 – fundername: NCRR NIH HHS grantid: 1S10RR23057 – fundername: NIAID NIH HHS grantid: AI094386 – fundername: NIGMS NIH HHS grantid: R01 GM071940 – fundername: NIH HHS grantid: 1S10OD018111 – fundername: NCRR NIH HHS grantid: S10 RR023057 |
GroupedDBID | --- --Z -DZ -ET -~X .55 .CO .XZ 00M 07C 0R~ 0WA 123 186 1OL 1VR 29M 2KS 2XV 39C 3V. 4.4 41X 53G 5RE 6TJ 70F 7RV 7X2 7X7 7XC 85S 88A 88E 88I 8AF 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ 8G5 8R4 8R5 8WZ 97F 97L A6W A7Z A8Z AAEEF AAHBH AAHTB AAIKC AAKAB AAKAS AAMNW AASDW AAYEP AAZLF ABDBF ABFSI ABIVO ABJCF ABJNI ABLJU ABOCM ABPEJ ABPPZ ABUWG ABVXF ABWJO ABZEH ACBEA ACBWK ACGFO ACGFS ACGOD ACIWK ACKOT ACMJI ACNCT ACPRK ACWUS ADBBV ADFRT ADUKH ADYSU ADZCM AENEX AFFNX AFKRA AFLOW AFRAH AFRQD AFSHS AGAYW AGEZK AGHSJ AGHTU AGNAY AGSOS AHMBA AHSBF AIDAL AIDUJ ALFFA ALIPV ALMA_UNASSIGNED_HOLDINGS AMTXH APEBS ARAPS ARMCB ARTTT ASPBG ATCPS ATWCN AVWKF AXYYD AZFZN AZQEC B-7 B0M BBNVY BCU BDKGC BEC BENPR BGLVJ BHPHI BIN BKEYQ BKKNO BKSAR BPHCQ BVXVI CCPQU CGR CJ0 CS3 CUY CVF D1I D1J D1K DO4 DU5 DWQXO E.- E.L EAD EAP EAS EAZ EBC EBD EBO EBS ECC ECM EE. EIF EJD EMB EMF EMH EMK EMOBN EPL EPS ESE ESN ESTFP ESX EX3 EXGXG F20 F5P FEDTE FQGFK FSGXE FYUFA GNUQQ GUQSH HCIFZ HG6 HMCUK HVGLF HZ~ I-F IAO ICQ IEA IEP IGS IH2 IHR INH INR IOF IPY ISR ITC K6- KB. KOO L6V L7B LK5 LK8 LSO M0K M0L M1P M2M M2O M2P M7P M7R M7S N9A NAPCQ NEJ NEPJS NPM O9- OBC OES OHH OMK OVD P-O P2P P62 PATMY PCBAR PDBOC PM3 PQQKQ PROAC PSQYO PSYQQ PTHSS PYCSY Q2X R05 RND RNS RNT RNTTT RXW S0X SC5 SHXYY SIXXV SJFOW SJN SNYQT SV3 TAE TAOOD TBHMF TDRGL TEORI TH9 TN5 TSG TUS TWZ U5U UIG UKHRP UKR UMD UQL VQA VVN WH7 WOW X7M XIH XKW XZL Y6R YAE YCJ YFH YNT YOC YQT YR2 YXB YZZ ZCA ZE2 ZKB ~02 ~7V ~88 ~8M ~KM AAYXX CITATION AADEA AAEXX ABEEJ ADFPY ADZGE AETEA NXXTH AADWK AAGJQ AAJMP AAYJO ABGIJ ACBMV ACBRV ACBYP ACIGE ACTTH ACVWB ADMDM ADQMX AEDAW AEFTE AFNRJ AGGBP AGPPL AHGBK AHPSJ AJDOV AMRJV I-U U1R XFK ZA5 AAPBV ABGFU ABPTK AEQTP 7QG 7QL 7QP 7QR 7SN 7SS 7ST 7T5 7TG 7TK 7TM 7TO 7U9 7XB 8FD 8FK C1K FR3 H94 K9. KL. M7N MBDVC P64 PQEST PQUKI Q9U RC3 SOI 7X8 5PM |
ID | FETCH-LOGICAL-c677t-b8b6b78f0ebc8ac19c73f0a21fb4b59fd9ab717b2ffae6f650a335542b41edb63 |
IEDL.DBID | BENPR |
ISSN | 0028-0836 |
IngestDate | Tue Sep 17 21:22:50 EDT 2024 Sat Aug 17 03:06:21 EDT 2024 Sat Oct 05 17:00:31 EDT 2024 Thu Feb 22 23:29:26 EST 2024 Fri Feb 02 04:43:05 EST 2024 Tue Dec 12 21:20:44 EST 2023 Fri Feb 02 04:26:51 EST 2024 Thu Aug 01 19:34:28 EDT 2024 Thu Aug 01 19:53:52 EDT 2024 Thu Sep 26 17:50:44 EDT 2024 Sat Sep 28 07:57:42 EDT 2024 |
IsDoiOpenAccess | true |
IsOpenAccess | true |
IsPeerReviewed | true |
IsScholarly | true |
Issue | 7579 |
Language | English |
License | Users may view, print, copy, and download text and data-mine the content in such documents, for the purposes of academic research, subject always to the full Conditions of use:http://www.nature.com/authors/editorial_policies/license.html#terms |
LinkModel | DirectLink |
MergedId | FETCHMERGED-LOGICAL-c677t-b8b6b78f0ebc8ac19c73f0a21fb4b59fd9ab717b2ffae6f650a335542b41edb63 |
Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
OpenAccessLink | https://pubmed.ncbi.nlm.nih.gov/PMC5086257 |
PMID | 26503045 |
PQID | 1738043005 |
PQPubID | 40569 |
PageCount | 4 |
ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_5086257 proquest_miscellaneous_1737480130 proquest_journals_1738043005 gale_infotracmisc_A436231752 gale_infotracgeneralonefile_A436231752 gale_infotraccpiq_436231752 gale_infotracacademiconefile_A436231752 gale_incontextgauss_ISR_A436231752 gale_incontextgauss_ATWCN_A436231752 crossref_primary_10_1038_nature15767 pubmed_primary_26503045 |
PublicationCentury | 2000 |
PublicationDate | 2015-11-26 |
PublicationDateYYYYMMDD | 2015-11-26 |
PublicationDate_xml | – month: 11 year: 2015 text: 2015-11-26 day: 26 |
PublicationDecade | 2010 |
PublicationPlace | England |
PublicationPlace_xml | – name: England – name: London |
PublicationTitle | Nature (London) |
PublicationTitleAlternate | Nature |
PublicationYear | 2015 |
Publisher | Nature Publishing Group |
Publisher_xml | – name: Nature Publishing Group |
References | 12464184 - Cell. 2002 Nov 27;111(5):733-45 12781660 - J Struct Biol. 2003 Jun;142(3):334-47 16452981 - Nature. 2006 Feb 2;439(7076):612-6 15572765 - Acta Crystallogr D Biol Crystallogr. 2004 Dec;60(Pt 12 Pt 1):2126-32 25243800 - Curr Opin Virol. 2014 Dec;9:31-8 15254177 - J Virol. 2004 Aug;78(15):8059-67 15890281 - J Struct Biol. 2005 Jun;150(3):332-9 21248043 - J Virol. 2011 Apr;85(7):3095-105 18238898 - Proc Natl Acad Sci U S A. 2008 Feb 12;105(6):1867-72 20308582 - Proc Natl Acad Sci U S A. 2010 Apr 6;107(14):6298-303 14608373 - Nat Struct Biol. 2003 Dec;10(12):1011-8 16709746 - Science. 2006 Jun 23;312(5781):1791-5 10801118 - Nature. 2000 Apr 27;404(6781):960-7 12827465 - Arch Virol. 2003 Jul;148(7):1357-68 10793759 - Nucleic Acids Res. 1974 Jun;1(6):809-22 23872434 - J Struct Biol. 2013 Sep;183(3):377-88 23089332 - J Mol Biol. 2013 Jan 9;425(1):124-32 19000820 - Structure. 2008 Nov 12;16(11):1678-88 21565700 - Structure. 2011 May 11;19(5):652-61 1848156 - Cell. 1991 Mar 8;64(5):1007-15 15264254 - J Comput Chem. 2004 Oct;25(13):1605-12 23000701 - J Struct Biol. 2012 Dec;180(3):519-30 18449192 - Nature. 2008 May 15;453(7193):415-9 17095250 - J Struct Biol. 2007 May;158(2):156-64 14613938 - J Biol Chem. 2004 Feb 6;279(6):4394-403 20124702 - Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):213-21 9814712 - Cell. 1998 Oct 30;95(3):431-7 10338212 - Cell. 1999 May 14;97(4):481-90 9311795 - J Virol. 1997 Oct;71(10):7220-6 17417654 - Nat Struct Mol Biol. 2007 May;14(5):449-51 22492979 - Proc Natl Acad Sci U S A. 2012 Apr 17;109(16):6118-23 12401805 - J Biol Chem. 2003 Jan 10;278(2):1094-100 2168982 - J Virol. 1990 Oct;64(10):4988-96 9774103 - Nature. 1998 Oct 1;395(6701):470-8 15113934 - J Virol. 2004 May;78(10):5528-30 15653727 - Biophys J. 2005 Apr;88(4):2737-44 8438576 - Virology. 1993 Mar;193(1):356-66 26240998 - Elife. 2015 Aug 04;4:e07901 9371626 - J Virol. 1997 Dec;71(12):9618-26 12036315 - Virology. 2002 Apr 25;296(1):24-38 19914821 - Curr Opin Struct Biol. 2009 Dec;19(6):746-51 17064936 - J Struct Biol. 2007 Feb;157(2):356-64 J Abels (BFnature15767_CR13) 2005; 88 X Zhang (BFnature15767_CR36) 2008; 105 JT Patton (BFnature15767_CR23) 1997; 71 Y Tao (BFnature15767_CR43) 1998; 95 X Yu (BFnature15767_CR35) 2011; 19 F Booy (BFnature15767_CR41) 1991; 64 BFnature15767_CR1 P Emsley (BFnature15767_CR44) 2004; 60 JM Grimes (BFnature15767_CR29) 1998; 395 JA Mindell (BFnature15767_CR32) 2003; 142 CL Luongo (BFnature15767_CR22) 2002; 296 Q Xia (BFnature15767_CR11) 2003; 278 BFnature15767_CR45 X Zhang (BFnature15767_CR9) 2003; 10 Y Tao (BFnature15767_CR2) 2002; 111 KH Choi (BFnature15767_CR19) 2009; 19 EF Pettersen (BFnature15767_CR31) 2004; 25 BFnature15767_CR6 ML Nibert (BFnature15767_CR14) 2004; 78 EA Mansell (BFnature15767_CR24) 1990; 64 Y Furuichi (BFnature15767_CR7) 1974; 1 M Wolf (BFnature15767_CR37) 2010; 107 PD Adams (BFnature15767_CR38) 2010; 66 J Kim (BFnature15767_CR18) 2004; 279 EL Nason (BFnature15767_CR10) 2004; 78 JA Briggs (BFnature15767_CR40) 2005; 150 MC Starnes (BFnature15767_CR27) 1993; 193 P Gouet (BFnature15767_CR12) 1999; 97 CL Gridley (BFnature15767_CR25) 2014; 9 G Sutton (BFnature15767_CR16) 2007; 14 X Yu (BFnature15767_CR21) 2015; 4 SM McDonald (BFnature15767_CR26) 2011; 85 D Lyumkis (BFnature15767_CR33) 2013; 183 W Jiang (BFnature15767_CR4) 2006; 439 N Stauber (BFnature15767_CR17) 1997; 71 LF Estrozi (BFnature15767_CR8) 2013; 425 C Yang (BFnature15767_CR20) 2012; 109 X Lu (BFnature15767_CR3) 2008; 16 S Zhao (BFnature15767_CR15) 2003; 148 X Yu (BFnature15767_CR30) 2008; 453 Y Zhang (BFnature15767_CR42) 2007; 157 GC Lander (BFnature15767_CR5) 2006; 312 KM Reinisch (BFnature15767_CR28) 2000; 404 SH Scheres (BFnature15767_CR34) 2012; 180 JT Huiskonen (BFnature15767_CR39) 2007; 158 |
References_xml | – volume: 64 start-page: 4988 year: 1990 ident: BFnature15767_CR24 publication-title: J. Virol. doi: 10.1128/JVI.64.10.4988-4996.1990 contributor: fullname: EA Mansell – volume: 71 start-page: 7220 year: 1997 ident: BFnature15767_CR17 publication-title: J. Virol. doi: 10.1128/JVI.71.10.7220-7226.1997 contributor: fullname: N Stauber – volume: 85 start-page: 3095 year: 2011 ident: BFnature15767_CR26 publication-title: J. Virol. doi: 10.1128/JVI.02360-10 contributor: fullname: SM McDonald – volume: 296 start-page: 24 year: 2002 ident: BFnature15767_CR22 publication-title: Virology doi: 10.1006/viro.2001.1258 contributor: fullname: CL Luongo – volume: 404 start-page: 960 year: 2000 ident: BFnature15767_CR28 publication-title: Nature doi: 10.1038/35010041 contributor: fullname: KM Reinisch – volume: 278 start-page: 1094 year: 2003 ident: BFnature15767_CR11 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M205964200 contributor: fullname: Q Xia – volume: 193 start-page: 356 year: 1993 ident: BFnature15767_CR27 publication-title: Virology doi: 10.1006/viro.1993.1132 contributor: fullname: MC Starnes – volume: 158 start-page: 156 year: 2007 ident: BFnature15767_CR39 publication-title: J. Struct. Biol. doi: 10.1016/j.jsb.2006.08.021 contributor: fullname: JT Huiskonen – ident: BFnature15767_CR1 – volume: 97 start-page: 481 year: 1999 ident: BFnature15767_CR12 publication-title: Cell doi: 10.1016/S0092-8674(00)80758-8 contributor: fullname: P Gouet – volume: 64 start-page: 1007 year: 1991 ident: BFnature15767_CR41 publication-title: Cell doi: 10.1016/0092-8674(91)90324-R contributor: fullname: F Booy – volume: 111 start-page: 733 year: 2002 ident: BFnature15767_CR2 publication-title: Cell doi: 10.1016/S0092-8674(02)01110-8 contributor: fullname: Y Tao – volume: 88 start-page: 2737 year: 2005 ident: BFnature15767_CR13 publication-title: Biophys. J. doi: 10.1529/biophysj.104.052811 contributor: fullname: J Abels – volume: 425 start-page: 124 year: 2013 ident: BFnature15767_CR8 publication-title: J. Mol. Biol. doi: 10.1016/j.jmb.2012.10.011 contributor: fullname: LF Estrozi – ident: BFnature15767_CR45 doi: 10.7554/eLife.01299 – volume: 107 start-page: 6298 year: 2010 ident: BFnature15767_CR37 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.0914604107 contributor: fullname: M Wolf – volume: 1 start-page: 809 year: 1974 ident: BFnature15767_CR7 publication-title: Nucleic Acids Res. doi: 10.1093/nar/1.6.809 contributor: fullname: Y Furuichi – volume: 66 start-page: 213 year: 2010 ident: BFnature15767_CR38 publication-title: Acta Crystallogr. D doi: 10.1107/S0907444909052925 contributor: fullname: PD Adams – volume: 4 start-page: e07901 year: 2015 ident: BFnature15767_CR21 publication-title: Elife doi: 10.7554/eLife.07901 contributor: fullname: X Yu – volume: 105 start-page: 1867 year: 2008 ident: BFnature15767_CR36 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.0711623105 contributor: fullname: X Zhang – volume: 25 start-page: 1605 year: 2004 ident: BFnature15767_CR31 publication-title: J. Comput. Chem. doi: 10.1002/jcc.20084 contributor: fullname: EF Pettersen – volume: 95 start-page: 431 year: 1998 ident: BFnature15767_CR43 publication-title: Cell doi: 10.1016/S0092-8674(00)81773-0 contributor: fullname: Y Tao – volume: 439 start-page: 612 year: 2006 ident: BFnature15767_CR4 publication-title: Nature doi: 10.1038/nature04487 contributor: fullname: W Jiang – volume: 312 start-page: 1791 year: 2006 ident: BFnature15767_CR5 publication-title: Science doi: 10.1126/science.1127981 contributor: fullname: GC Lander – volume: 279 start-page: 4394 year: 2004 ident: BFnature15767_CR18 publication-title: J. Biol. Chem. doi: 10.1074/jbc.M308637200 contributor: fullname: J Kim – ident: BFnature15767_CR6 – volume: 16 start-page: 1678 year: 2008 ident: BFnature15767_CR3 publication-title: Structure doi: 10.1016/j.str.2008.09.006 contributor: fullname: X Lu – volume: 180 start-page: 519 year: 2012 ident: BFnature15767_CR34 publication-title: J. Struct. Biol. doi: 10.1016/j.jsb.2012.09.006 contributor: fullname: SH Scheres – volume: 9 start-page: 31 year: 2014 ident: BFnature15767_CR25 publication-title: Curr. Opin. Virol. doi: 10.1016/j.coviro.2014.08.008 contributor: fullname: CL Gridley – volume: 142 start-page: 334 year: 2003 ident: BFnature15767_CR32 publication-title: J. Struct. Biol. doi: 10.1016/S1047-8477(03)00069-8 contributor: fullname: JA Mindell – volume: 183 start-page: 377 year: 2013 ident: BFnature15767_CR33 publication-title: J. Struct. Biol. doi: 10.1016/j.jsb.2013.07.005 contributor: fullname: D Lyumkis – volume: 60 start-page: 2126 year: 2004 ident: BFnature15767_CR44 publication-title: Acta Crystallogr. D doi: 10.1107/S0907444904019158 contributor: fullname: P Emsley – volume: 78 start-page: 8059 year: 2004 ident: BFnature15767_CR10 publication-title: J. Virol. doi: 10.1128/JVI.78.15.8059-8067.2004 contributor: fullname: EL Nason – volume: 453 start-page: 415 year: 2008 ident: BFnature15767_CR30 publication-title: Nature doi: 10.1038/nature06893 contributor: fullname: X Yu – volume: 78 start-page: 5528 year: 2004 ident: BFnature15767_CR14 publication-title: J. Virol. doi: 10.1128/JVI.78.10.5528-5530.2004 contributor: fullname: ML Nibert – volume: 395 start-page: 470 year: 1998 ident: BFnature15767_CR29 publication-title: Nature doi: 10.1038/26694 contributor: fullname: JM Grimes – volume: 150 start-page: 332 year: 2005 ident: BFnature15767_CR40 publication-title: J. Struct. Biol. doi: 10.1016/j.jsb.2005.03.009 contributor: fullname: JA Briggs – volume: 109 start-page: 6118 year: 2012 ident: BFnature15767_CR20 publication-title: Proc. Natl Acad. Sci. USA doi: 10.1073/pnas.1200206109 contributor: fullname: C Yang – volume: 14 start-page: 449 year: 2007 ident: BFnature15767_CR16 publication-title: Nature Struct. Mol. Biol. doi: 10.1038/nsmb1225 contributor: fullname: G Sutton – volume: 148 start-page: 1357 year: 2003 ident: BFnature15767_CR15 publication-title: Arch. Virol. doi: 10.1007/s00705-003-0103-z contributor: fullname: S Zhao – volume: 71 start-page: 9618 year: 1997 ident: BFnature15767_CR23 publication-title: J. Virol. doi: 10.1128/JVI.71.12.9618-9626.1997 contributor: fullname: JT Patton – volume: 10 start-page: 1011 year: 2003 ident: BFnature15767_CR9 publication-title: Nature Struct. Mol. Biol. doi: 10.1038/nsb1009 contributor: fullname: X Zhang – volume: 19 start-page: 652 year: 2011 ident: BFnature15767_CR35 publication-title: Structure doi: 10.1016/j.str.2011.03.003 contributor: fullname: X Yu – volume: 19 start-page: 746 year: 2009 ident: BFnature15767_CR19 publication-title: Curr. Opin. Struct. Biol. doi: 10.1016/j.sbi.2009.10.015 contributor: fullname: KH Choi – volume: 157 start-page: 356 year: 2007 ident: BFnature15767_CR42 publication-title: J. Struct. Biol. doi: 10.1016/j.jsb.2006.09.002 contributor: fullname: Y Zhang |
SSID | ssj0005174 |
Score | 2.5149665 |
Snippet | Viruses in the Reoviridae, like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of... Viruses in the Reoviridae , like the triple-shelled human rotavirus and the single-shelled insect cytoplasmic polyhedrosis virus (CPV), all package a genome of... |
SourceID | pubmedcentral proquest gale crossref pubmed |
SourceType | Open Access Repository Aggregation Database Index Database |
StartPage | 531 |
SubjectTerms | Binding sites Capsid Proteins - chemistry Capsid Proteins - metabolism Capsid Proteins - ultrastructure Catalytic Domain Cryoelectron Microscopy Crystal structure Enzymes Genetic aspects Genome, Viral - genetics Genomes Kinases Models, Molecular Multienzyme Complexes - chemistry Multienzyme Complexes - metabolism Multienzyme Complexes - ultrastructure Nucleoside-Triphosphatase - metabolism Nucleoside-Triphosphatase - ultrastructure Protein Subunits - chemistry Protein Subunits - metabolism Proteins Reoviridae - enzymology Reoviridae - genetics Reoviridae - ultrastructure Reoviruses RNA RNA polymerase RNA polymerases RNA Replicase - chemistry RNA Replicase - metabolism RNA Replicase - ultrastructure RNA, Double-Stranded - genetics RNA, Double-Stranded - ultrastructure RNA, Messenger - biosynthesis RNA, Messenger - genetics RNA, Messenger - ultrastructure RNA, Viral - biosynthesis RNA, Viral - genetics RNA, Viral - ultrastructure Synthesis Templates, Genetic Transcription, Genetic Viruses |
Title | In situ structures of the segmented genome and RNA polymerase complex inside a dsRNA virus |
URI | https://www.ncbi.nlm.nih.gov/pubmed/26503045 https://www.proquest.com/docview/1738043005/abstract/ https://search.proquest.com/docview/1737480130 https://pubmed.ncbi.nlm.nih.gov/PMC5086257 |
Volume | 527 |
hasFullText | 1 |
inHoldings | 1 |
isFullTextHit | |
isPrint | |
link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwELf2ISReEBsfKxuTQRMfD1ETx4ndJ1SqlQ6JCpVNVLxE_iyVIOlIi-C_5y5Ju2aaePHL_WI59tm-s-9-JuQMZplliY0CYXgYcOV1oLU2GDQeJpbzHjOYKPxpnI6u-MdpMt0ho3UuDIZVrtfEaqG2hcEz8m4kYon8VGHSVRpPAcyy-25xHeD7UXjP2jymsUv2WcTxwnb__fn48-Qm3OMWI3OTqxfGsltTaEZgeYvW7nR7jd7apNoBlFs70vAhedCYkrRfj_0B2XH5IblXhXSa8pAcNNO2pG8abum3j8i3i5zCf6xozRsLDSpp4SlYgbR0s4qg01Lkbf3pqMotnYz7dFH8-ItHV6WjVQC6-0Pn1SufVFFbIuL3_NeqfEyuhueXg1HQPK8QmFSIZaClTrWQPnTaSGWinhGxDxWLvOY66XnbUxqcPc28Vy71YMqpGK0TpnnkrE7jJ2QvL3J3RCizEkwJwyPVA3fPORXG4O06Cb5dnHLJOuRs3a3ZombRyKrb71hmW70PMOzyDHkpcgx8malVWWb9y6-DcdbnsNeitQO1vbwLdvFl0gK9bkC-QG1RTboBNBgZr1rI4xbSLObX2Zb0VUs6q0fsrmpOWkCYpqYtXutR1iwTZXaj1B3yYiPGLzH0LXfFqsII5PiJww55Wqvdpg8ZDApedXeIaCnkBoDk4W1JPv9ekYgn6Msm4tn_m3VM7oOFmGDyJUtPyB7opnsOVthSn5JdMRVQykGE5fDDaTPl_gEejDPi |
link.rule.ids | 230,315,786,790,891,12083,12250,12792,21416,27957,27958,31754,31755,33301,33302,33408,33409,33779,33780,43345,43614,43635,43840,74102,74371,74392,74659 |
linkProvider | ProQuest |
linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3Pb9MwFLZgCMEFsfGrbIBBE7BDtCR24vSEqompha2H0YmKS2Q7dqkESYdXBP897yVuV08T5_fFcuxn-z3782dC9mGUVWlWJZHQPI64tCpSSmkkjcdZxXk_1XhR-HScD8_5p2k29RtuztMqV3NiO1FXjcY98sNEsAL1qeLsw-Iiwlej8HTVP6Fxm9zhjHGk9ImpuKJ4XFNh9vfzYlYcdrKZCUTbIliRrs_LGwtTSJrcWIWOH5IHPnykg66_t8ktU--Quy2NU7sdsu2HqqPvvZ70wSPybVRT-Isl7bRioUKONpZC5EedmbWinBVFrdafhsq6omfjAV00P_7idpUztCWdmz903r7sSSWtHCJ-z38t3WNyfvxxcjSM_JMKkc6FuIxUoXIlChsbpQupk74WzMYyTaziKuvbqi8VJHgqtVaa3EL4JhlGJKniialUzp6QrbqpzTNC06qA8EHzRPYhxTNGxgwyXFNAPsdyXqQ9sr9q1nLRKWeU7Yk3K8qN1gcYNnmJWhQ1kl1mculcOZh8PRqXAw7rK0Y4UNqbm2CjL2cB6J0H2Qb6SEt_xQAqjCpXAXI3QOrF_KLcsL4NrLOux24qZi8AwtDUoXnlR6WfGlx55cg98nptxi-R7labZtliBOr6sLhHnnZut27DFDoFj7d7RAQOuQagYHhoqeffW-HwDPPXTDz_f7VekXvDyelJeTIaf94l9yFCzPDyZZrvkS3wU_MCorBL9bIdav8AkuEvFQ |
linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwfV3db9MwELdgE4gXxMZX2QCDJj4eoubDiZMnVMaqlY9qKps28RLZjl0qQdLhFcF_z13idvU08Xy_WI7vzr6zzz8TsgdeVsVpFQVcsTBgwshASqmwaDxMK8aKWOFF4S_j7PCEfTxLz1z9k3Vllcs5sZ2oq0bhHnk_4kmO_FRh2jeuLOLow_Dd_DzAF6TwpNU9p3GTbHKWpWDhm-8PxkeTy4KPK5zM7rZemOT9jkQzgtibe-vT1Vl6bZnySyjX1qThPXLXBZN00Gl_i9zQ9Ta51RZ1KrtNtpzjWvrGsUu_vU--jWoK_7GgHXMsdMjSxlCIA6nV05ais6LI3PpTU1FXdDIe0Hnz4y9uXllN2xJ0_YfO2nc-qaCVRcTv2a-FfUBOhgfH-4eBe2AhUBnnF4HMZSZ5bkItVS5UVCiemFDEkZFMpoWpCiEh3ZOxMUJnBoI5kWB8EksW6UpmyUOyUTe1fkxoXOUQTCgWiQISPq1FmEC-q3PI7pKM5XGP7C2HtZx3PBple_6d5OXa6AMMh7xEZooadTwVC2vLwfHp_rgcMFhtMd6B1l5eBxt9nXig1w5kGtCREu7CAXQYOa885I6HVPPZebkmfeVJp53Grmtm1wOCoypfvLSj0k0Utrw06x55sRLjl1j8Vutm0WI4svwkYY886sxuNYYxKAUPu3uEewa5AiB9uC-pZ99bGvEUs9mUP_l_t56T2-Bn5efR-NMOuQPhYoo3MeNsl2yAmeqnEJJdyGfO1_4BMa00uA |
openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+situ+structures+of+the+segmented+genome+and+RNA+polymerase+complex+inside+a+dsRNA+virus&rft.jtitle=Nature+%28London%29&rft.au=Zhang%2C+Xing&rft.au=Ding%2C+Ke&rft.au=Yu%2C+Xuekui&rft.au=Chang%2C+Winston&rft.date=2015-11-26&rft.pub=Nature+Publishing+Group&rft.issn=0028-0836&rft.eissn=1476-4687&rft.spage=531&rft_id=info:doi/10.1038%2Fnature15767&rft.externalDBID=ATWCN&rft.externalDocID=A436231752 |
thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0028-0836&client=summon |
thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0028-0836&client=summon |
thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0028-0836&client=summon |