Genome-wide identification of zero nucleotide recursive splicing in Drosophila

• Published in: Nature (London) Vol. 521; no. 7552; pp. 376 - 379
• Main Authors: Duff, Michael O., Olson, Sara, Wei, Xintao, Garrett, Sandra C., Osman, Ahmad, Bolisetty, Mohan, Plocik, Alex, Celniker, Susan E., Graveley, Brenton R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 21.05.2015; Nature Publishing Group
• Subjects: 45/91; 631/337/1645/1792; 631/45/500; 64/24; Animals; Base Sequence; Cells, Cultured; Drosophila; Drosophila melanogaster - genetics; Exons - genetics; Female; Gene expression; Genes, Insect - genetics; Genetic aspects; Genetic research; Genome, Insect - genetics; Genomes; Genomics; Humanities and Social Sciences; Humans; Insects; Introns - genetics; letter; Male; multidisciplinary; Nuclear Proteins - deficiency; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nucleotides - genetics; Reproducibility of Results; Ribonucleoproteins - deficiency; Ribonucleoproteins - genetics; Ribonucleoproteins - metabolism; RNA Splice Sites - genetics; RNA splicing; RNA Splicing - genetics; Science; Splicing Factor U2AF

In flies, some introns contain internal splice sites that cause ‘recursive splicing’, a multi-step removal of a single intron; this study demonstrates that the scope of this regulatory mechanism is much more extensive in flies than had been appreciated, and provides details about the recursive splic...