Reprogramming of murine and human somatic cells using a single polycistronic vector

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 1; pp. 157 - 162
• Main Authors: Carey, Bryce W, Markoulaki, Styliani, Hanna, Jacob, Saha, Kris, Gao, Qing, Mitalipova, Maisam, Jaenisch, Rudolf
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 06.01.2009; National Acad Sciences
• Subjects: adults; Animals; B lymphocytes; Biological Sciences; Cell lines; Cells; Cells, Cultured; Cellular Reprogramming - genetics; Cloning; Embryonic stem cells; Embryos; ethics; Fibroblasts; Fibroblasts - cytology; Genetic Vectors - genetics; Human subjects; humans; Induced pluripotent stem cells; insertional mutagenesis; keratinocytes; Keratinocytes - cytology; Kruppel-Like Transcription Factors - genetics; methodology; Mice; Neural stem cells; Octamer Transcription Factor-3 - genetics; Peptides; Pluripotent Stem Cells - cytology; Proto-Oncogene Proteins c-myc - genetics; Rodents; Somatic cells; SOXB1 Transcription Factors - genetics; Stem cells; Transfection - methods; Transgenes - genetics; Viruses

Directed reprogramming of somatic cells by defined factors provides a novel method for the generation of patient-specific stem cells with the potential to bypass both the practical and ethical concerns associated with somatic cell nuclear transfer (SCNT) and human embryonic stem (hES) cells. Althoug...