Tissue factor–bearing exosome secretion from human mechanically stimulated bronchial epithelial cells in vitro and in vivo

• Published in: Journal of allergy and clinical immunology Vol. 130; no. 6; pp. 1375 - 1383
• Main Authors: Park, Jin-Ah, Sharif, Asma S., Tschumperlin, Daniel J., Lau, Laurie, Limbrey, Rachel, Howarth, Peter, Drazen, Jeffrey M.
• Format: Journal Article
• Language: English
• Published: New York, NY Mosby, Inc 01.12.2012; Elsevier; Elsevier Limited
• Subjects: Adult; Age; Aged; Airway management; Airway Remodeling; Allergy and Immunology; Angiogenesis; Asthma; Asthma - immunology; Asthma - pathology; Atmospheric pressure; Biological and medical sciences; Biopsy; blood coagulation; Bronchi - immunology; Bronchi - pathology; bronchial epithelium; Bronchoalveolar Lavage Fluid - immunology; bronchoconstriction; Bronchoconstriction - immunology; Cells, Cultured; Cellular Microenvironment - immunology; Chronic obstructive pulmonary disease, asthma; epithelial cells; Epithelial Cells - immunology; epithelium; exosomes; Exosomes - immunology; Fundamental and applied biological sciences. Psychology; Fundamental immunology; gene expression regulation; Humans; immunohistochemistry; Immunopathology; in vivo studies; Kinases; mechanotransduction; Mechanotransduction, Cellular; Medical sciences; messenger RNA; Microscopy; Middle Aged; patients; Pneumology; Proteins; Respiratory distress syndrome; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; secretion; Thromboplastin - genetics; Thromboplastin - metabolism; tissue factor; ultracentrifugation; water; Young Adult; NHBECs; tissue factor; mechanotransduction; BALF; PKC; CM; bronchoconstriction; Bis 1; EBBs; Asthma; TF; exosomes; bronchial epithelium; GAPDH; TEM; ALI; Protein kinase C; Bisindoylmaleimide 1; Normal human bronchial epithelial cells; Conditioned media; Air-liquid interface; Glyceraldehyde-3-phosphate; Transmission electron microscopy; Bronchoalveolar lavage fluid; Endobronchial biopsies; Human; Lung disease; Immunopathology; Respiratory disease; Secretion; Tissue factor; Bronchus; Epithelium; Respiratory system; In vitro; Respiratory tract; In vivo; Bronchoconstriction; Immunology; Exosome; Bronchus disease; Epithelial cell; Obstructive pulmonary disease

Tissue factor (TF), a primary initiator of blood coagulation, also plays a pivotal role in angiogenesis. TF expression in the airways is associated with asthma, a disease characterized in part by subepithelial angiogenesis. To determine potential sources of TF and the mechanisms of its availability...