Method for estimation of toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice

We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the inject...

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Published inJournal of Veterinary Medical Science Vol. 66; no. 12; pp. 1591 - 1593
Main Authors Takikawa, N. (Kitasato Inst., Tokyo (Japan)), Kawahara, K, Sawata, A, Kume, K
Format Journal Article
LanguageEnglish
Published Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.12.2004
Japan Science and Technology Agency
Subjects
Animals
Bacterial Vaccines - chemistry
endotoxin
ENDOTOXINS
Endotoxins - analysis
Galactosamine
Lethal Dose 50
Limulus
Limulus Test - methods
METHODS
MICE
Mice - immunology
Mice, Inbred C3H
SALMONELLA
vaccine
VACCINES
Online AccessGet full text
ISSN0916-7250
1347-7439
DOI10.1292/jvms.66.1591

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Abstract We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD sub(50) in the mouse safety test (r=O.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
AbstractList We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD sub(50) in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD50 in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD sub(50) in the mouse safety test (r=O.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD50 in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD50 in the mouse safety test (r=0.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.
Author Takikawa, N. (Kitasato Inst., Tokyo (Japan))
Kawahara, K
Sawata, A
Kume, K
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Cites_doi 10.1073/pnas.76.11.5939
10.1128/IAI.51.3.891-895.1986
10.3181/00379727-131-34059
10.1292/jvms.59.927
10.2460/javma.1992.200.12.1894
10.1007/BF02214982
10.4049/jimmunol.84.2.189
10.1128/JB.93.4.1463-1464.1967
10.1128/IAI.59.6.2110-2115.1991
10.1126/science.282.5396.2085
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References 14. Rifkind, D. 1967. J. Bacteriol. 93: 1463-1464.
1. Cullor, J. S. 1984. J. Am. Vet. Med. Assoc. 200: 1894-1902.
3. Freudenberg, M. A. and Galanos, C. 1991. Infect. Immun. 59: 2110-2115.
6. Hussaini, S. N. and Ready, R. A. 1981. Vet. Res. Commun. 5: 171-175.
11. Poltorak, A., He, X., Smirnova, I., Liu, MY., Van Huffel, C., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C., Freudenberg, M., Ricciardi-Castagnoli, P., Layton, B. and Beuter, B. 1998. Science 282: 2085-2088.
8. Kreeftenberg, J. G., Loggen, H. G., Ramshort, J. D. and Beuvery, E. C. 1976. Develop. Boil. Standard. 34: 15-20.
12. Rieder, R. F. and Thomas, L. 1960. J. Immunol. 84: 189-193.
5. Grisman, S. E. and Hornick, R. B. 1969. Proc. Soc. Exp. Biol. Med. 131: 1154-1158.
4. Galanos, C., Freuenberg, M. A. and Reutter, W. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 5939-5943.
2. Freudenberg, M. A., Keppler, D. and Galanos, C. 1986. Infect. Immun. 51: 891-895.
13. Rietsched, E., Brade, L., Lindner, B. and Zahringer, U. 1999. pp. 3-4. In: Bacterial Endotoxic: Lipopolysaccharides vol.I (Morrison, D. C. and Ryan, J. L. eds.), CRC Press, Boca Raton, U. S. A.
7. Hussaini, S. N. and Sawtell, J. A. A. 1986. Develop. Biol. Standard. 64: 261-269.
10. Ohtsuka, H., Ohki, K., Tanaka, T., Tajima, M., Yoshino, T. and Takahashi, K. 1997. J. Vet. Med. Sci. 59: 927-929.
9. Mullenax, C. H., Keeler, R. F. and Allison, M. J. 1966. Am. J. Vet. Res. 27: 857-868.
Cullor, J. S. (1) 1984; 200
Poltorak, A., He, X., Smirnova, I. (11) 1998; 282
Rietsched, E., Brade, L., Lindner (13) 1999
Hussaini, S. N. and Ready, R. A. (6) 1981; 5
Kreeftenberg, J. G., Loggen, H. G. (8) 1976; 34
Mullenax, C. H., Keeler, R. F. and (9) 1966; 27
Freudenberg, M. A., Keppler, D. and (2) 1986; 51
Hussaini, S. N. and Sawtell, J. A. (7) 1986; 64
reudenberg, M. A. and Galanos, C. (3) 1991; 59
Grisman, S. E. and Hornick, R. B. (5) 1969; 131
Rifkind, D. (14) 1967; 93
Galanos, C., Freuenberg, M. A. and (4) 1979; 76
Rieder, R. F. and Thomas, L. (12) 1960; 84
10
References_xml – reference: 12. Rieder, R. F. and Thomas, L. 1960. J. Immunol. 84: 189-193.
– reference: 13. Rietsched, E., Brade, L., Lindner, B. and Zahringer, U. 1999. pp. 3-4. In: Bacterial Endotoxic: Lipopolysaccharides vol.I (Morrison, D. C. and Ryan, J. L. eds.), CRC Press, Boca Raton, U. S. A.
– reference: 2. Freudenberg, M. A., Keppler, D. and Galanos, C. 1986. Infect. Immun. 51: 891-895.
– reference: 4. Galanos, C., Freuenberg, M. A. and Reutter, W. 1979. Proc. Natl. Acad. Sci. U.S.A. 76: 5939-5943.
– reference: 7. Hussaini, S. N. and Sawtell, J. A. A. 1986. Develop. Biol. Standard. 64: 261-269.
– reference: 8. Kreeftenberg, J. G., Loggen, H. G., Ramshort, J. D. and Beuvery, E. C. 1976. Develop. Boil. Standard. 34: 15-20.
– reference: 5. Grisman, S. E. and Hornick, R. B. 1969. Proc. Soc. Exp. Biol. Med. 131: 1154-1158.
– reference: 10. Ohtsuka, H., Ohki, K., Tanaka, T., Tajima, M., Yoshino, T. and Takahashi, K. 1997. J. Vet. Med. Sci. 59: 927-929.
– reference: 3. Freudenberg, M. A. and Galanos, C. 1991. Infect. Immun. 59: 2110-2115.
– reference: 9. Mullenax, C. H., Keeler, R. F. and Allison, M. J. 1966. Am. J. Vet. Res. 27: 857-868.
– reference: 1. Cullor, J. S. 1984. J. Am. Vet. Med. Assoc. 200: 1894-1902.
– reference: 11. Poltorak, A., He, X., Smirnova, I., Liu, MY., Van Huffel, C., Du, X., Birdwell, D., Alejos, E., Silva, M., Galanos, C., Freudenberg, M., Ricciardi-Castagnoli, P., Layton, B. and Beuter, B. 1998. Science 282: 2085-2088.
– reference: 6. Hussaini, S. N. and Ready, R. A. 1981. Vet. Res. Commun. 5: 171-175.
– reference: 14. Rifkind, D. 1967. J. Bacteriol. 93: 1463-1464.
– volume: 27
  start-page: 857
  issn: 0002-9645
  year: 1966
  ident: 9
  publication-title: Am. J. Vet. Res.
– volume: 76
  start-page: 5939
  issn: 0027-8424
  year: 1979
  ident: 4
  publication-title: Proc. Natl. Acad. Sci. U.S.A.
  doi: 10.1073/pnas.76.11.5939
– volume: 51
  start-page: 891
  issn: 0019-9567
  year: 1986
  ident: 2
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.51.3.891-895.1986
– volume: 131
  start-page: 1154
  issn: 0037-9727
  year: 1969
  ident: 5
  publication-title: Proc. Soc. Exp. Biol. Med.
  doi: 10.3181/00379727-131-34059
– volume: 64
  start-page: 261
  issn: 0301-5149
  year: 1986
  ident: 7
  publication-title: Develop. Biol. Standard.
– ident: 10
  doi: 10.1292/jvms.59.927
– volume: 200
  start-page: 1894
  issn: 0003-1488
  year: 1984
  ident: 1
  publication-title: J. Am. Vet. Med. Assoc.
  doi: 10.2460/javma.1992.200.12.1894
– volume: 5
  start-page: 171
  issn: 0165-7380
  year: 1981
  ident: 6
  publication-title: Vet. Res. Commun.
  doi: 10.1007/BF02214982
– volume: 84
  start-page: 189
  issn: 0022-1767
  year: 1960
  ident: 12
  publication-title: J. Immunol.
  doi: 10.4049/jimmunol.84.2.189
– start-page: 3
  year: 1999
  ident: 13
– volume: 93
  start-page: 1463
  issn: 0021-9193
  year: 1967
  ident: 14
  publication-title: J. Bacteriol.
  doi: 10.1128/JB.93.4.1463-1464.1967
– volume: 59
  start-page: 2110
  issn: 0019-9567
  year: 1991
  ident: 3
  publication-title: Infect. Immun.
  doi: 10.1128/IAI.59.6.2110-2115.1991
– volume: 34
  start-page: 15
  issn: 0012-1606
  year: 1976
  ident: 8
  publication-title: Develop. Boil. Standard.
– volume: 282
  start-page: 2085
  issn: 0036-8075
  year: 1998
  ident: 11
  publication-title: Science
  doi: 10.1126/science.282.5396.2085
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SubjectTerms Animals
Bacterial Vaccines - chemistry
endotoxin
ENDOTOXINS
Endotoxins - analysis
Galactosamine
Lethal Dose 50
Limulus
Limulus Test - methods
METHODS
MICE
Mice - immunology
Mice, Inbred C3H
SALMONELLA
vaccine
VACCINES
Title Method for estimation of toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice
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