Method for estimation of toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice

• Published in: Journal of Veterinary Medical Science Vol. 66; no. 12; pp. 1591 - 1593
• Main Authors: Takikawa, N. (Kitasato Inst., Tokyo (Japan)), Kawahara, K, Sawata, A, Kume, K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01.12.2004; Japan Science and Technology Agency
• Subjects: Animals; Bacterial Vaccines - chemistry; endotoxin; ENDOTOXINS; Endotoxins - analysis; Galactosamine; Lethal Dose 50; Limulus; Limulus Test - methods; METHODS; MICE; Mice - immunology; Mice, Inbred C3H; SALMONELLA; vaccine; VACCINES
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.66.1591

We developed a method to estimate the content of the toxic endotoxin in inactivated Salmonella vaccine in D-galactosamine-sensitized mice. Ten-fold serially diluted vaccines were injected intraperitoneally into D-galactosamine-sensitized mice. Lethality in the mice was judged 3 days after the injection. The best result was obtained when C3H/HeN mice were used for the test. Correlation was observed between the endotoxin content measured by Limulus amoebocyte lysate assay and the LD sub(50) in the mouse safety test (r=O.81). These results suggested that this test could be applied to the estimation of endotoxin content in inactivated vaccines of Salmonella.