Analysis of essential pathways for self-renewal in common marmoset embryonic stem cells

• Published in: FEBS open bio Vol. 4; no. 1; pp. 213 - 219
• Main Authors: Nii, Takenobu, Marumoto, Tomotoshi, Kawano, Hirotaka, Yamaguchi, Saori, Liao, Jiyuan, Okada, Michiyo, Sasaki, Erika, Miura, Yoshie, Tani, Kenzaburo
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 01.01.2014; John Wiley & Sons, Inc; Elsevier; Wiley
• Subjects: 1-Phosphatidylinositol 3-kinase; AKT protein; bFGF; Biotechnology; Cell culture; Cell self-renewal; Common marmoset; Embryo cells; Embryo fibroblasts; Embryonic stem cells; Fibroblast growth factor 2; Fibroblasts; Flow cytometry; Gene expression; Growth factors; Kinases; Laboratories; Leukemia; Leukemia inhibitory factor; Polymerase chain reaction; Proteins; Regenerative medicine; Self-renewal; Software; Stem cell transplantation; Stem cells; TGFβ; Transforming growth factor-b; Variance analysis; United States > US; Japan; MEFs; SMAD2/3; STAT3; LIF; AKT; CM; KSR; Embryonic stem cells; bFGF; FCM; iPSCs; MEK; Self-renewal; PI3K; Common marmoset; JAK; RT-PCR; TGFβ; EpiSCs; EB; ESCs; ERK; EB, embryoid body; EpiSCs, epiblast stem cells; JAK, janus kinase; MEK, mitogen-activated protein/extracellular signal-regulated kinase kinase; SMAD2/3, mothers against decapentaplegic homolog 2/3; KSR, knockout serum replacement; TGFβ, transforming growth factor β; STAT3, signal transducer and activator of transcription 3; CM, common marmoset; FCM, flow cytometry; ERK, extracellular signal-regulated kinase; AKT, protein kinase B; iPSCs, induced pluripotent stem cells; bFGF, basic fibroblast growth factor; LIF, leukemia inhibitory factor; MEFs, mouse embryonic fibroblasts; ESCs, embryonic stem cells; PI3K, phosphatidylinositol-3-kinase; RT-PCR, reverse transcription-polymerase chain reaction

•Self-renewal of common marmoset embryonic stem cells (CM ESCs) is promoted by bFGF.•bFGF activates the PI3K-AKT pathway in CM ESCs on feeder cells.•bFGF and TGFβ in combination support culture of CM ESCs without feeder cells.•CM ESCs show phenotypes similar to those of human ESCs and mouse epiblast...