Telomere Dynamics in Human Cells Reprogrammed to Pluripotency
Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from old...
Saved in:
| Published in | PloS one Vol. 4; no. 12; p. e8124 |
|---|---|
| Main Authors | , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
United States
Public Library of Science
02.12.2009
Public Library of Science (PLoS) |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation."
We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres.
While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. |
|---|---|
| AbstractList | Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation."BACKGROUNDHuman induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation."We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres.METHODOLOGY/PRINCIPAL FINDINGSWe examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres.While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age.CONCLUSIONS/SIGNIFICANCEWhile these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. Background Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell arejuvenation.a Methodology/Principal Findings We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres. Conclusions/Significance While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. BACKGROUND:Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation." METHODOLOGY/PRINCIPAL FINDINGS:We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres. CONCLUSIONS/SIGNIFICANCE:While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell 'rejuvenation.' We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres. While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell "rejuvenation." We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres. While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. Background Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of autologous cells and tissues for therapeutic use. A question remains as to the biological age of IPSCs, in particular when isolated from older subjects. Studies of cloned animals indicate that somatic cells reprogrammed to pluripotency variably display telomere elongation, a common indicator of cell “rejuvenation.” Methodology/Principal Findings We examined telomere lengths in human skin fibroblasts isolated from younger and older subjects, fibroblasts converted to IPSCs, and IPSCs redifferentiated through teratoma formation and explant culture. In IPSCs analyzed at passage five (P5), telomeres were significantly elongated in 6/7 lines by >40% and approximated telomere lengths in human embryonic stem cells (hESCs). In cell lines derived from three IPSC-teratoma explants cultured to P5, two displayed telomeres shortened to lengths similar to input fibroblasts while the third line retained elongated telomeres. Conclusions/Significance While these results reveal some heterogeneity in the reprogramming process with respect to telomere length, human somatic cells reprogrammed to pluripotency generally displayed elongated telomeres that suggest that they will not age prematurely when isolated from subjects of essentially any age. |
| Audience | Academic |
| Author | Suhr, Steven T. Beyhan, Zeki Cibelli, Jose B. Chang, Eun Ah Rodriguez, Ramon M. Murthy, Shashanka Ross, Pablo J. Wang, Kai |
| AuthorAffiliation | 1 Cellular Reprogramming Laboratory, Department of Animal Science, Michigan State University, East Lansing, Michigan, United States of America University of Southern California, United States of America 2 Programa Andaluz de Terapia Celular y Medicina Regenerativa, Andalucía, Spain |
| AuthorAffiliation_xml | – name: University of Southern California, United States of America – name: 2 Programa Andaluz de Terapia Celular y Medicina Regenerativa, Andalucía, Spain – name: 1 Cellular Reprogramming Laboratory, Department of Animal Science, Michigan State University, East Lansing, Michigan, United States of America |
| Author_xml | – sequence: 1 givenname: Steven T. surname: Suhr fullname: Suhr, Steven T. – sequence: 2 givenname: Eun Ah surname: Chang fullname: Chang, Eun Ah – sequence: 3 givenname: Ramon M. surname: Rodriguez fullname: Rodriguez, Ramon M. – sequence: 4 givenname: Kai surname: Wang fullname: Wang, Kai – sequence: 5 givenname: Pablo J. surname: Ross fullname: Ross, Pablo J. – sequence: 6 givenname: Zeki surname: Beyhan fullname: Beyhan, Zeki – sequence: 7 givenname: Shashanka surname: Murthy fullname: Murthy, Shashanka – sequence: 8 givenname: Jose B. surname: Cibelli fullname: Cibelli, Jose B. |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/19956585$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNk2tr2zAUhs3oWNts_2BshkHHPiTTXXJhg5JdGih0dN2-CkWWExVZyiR7LP9-yqUlKaMrNrY5fs4rznvOOS4OfPCmKF5CMIKYw_c3oY9eudEih0cAAAEReVIcwQqjIUMAH-x8HxbHKd0AQLFg7FlxCKuKMiroUfHh2rjQmmjKT0uvWqtTaX153rfKl2PjXCqvzCKGWVRta-qyC-U310e7CJ3xevm8eNool8yL7XtQ_Pjy-Xp8Pry4_DoZn10MNeOIDDVGlAjNUKOQMmRaQVDXglY1B40gDCvNa66h4FORH4o2kNZIKAY4IxVpCB4Urze6CxeS3FaeJEQVQgDgfA-KyYaog7qRi2hbFZcyKCvXgRBnUsXOamfkVDWUGYI5pYBgbSowrYAQkCAKIVif9nF7Wj_NRWvju6jcnuj-H2_nchZ-S8QFwBBlgbdbgRh-9SZ1srVJZzeVN6FPkhNaZbCi_ydxbjWjVGTy5EESQcKzyewxIM7O4gy-uQf-29jRhpqp7J31Tcgl63zVJk9Lnr3G5vgZ4bl4TNay7_YSMtOZP91M9SnJyferx7OXP_fZkx12bpTr5im4vrPBp33w1W777vp2O_QZON0AOoaUommktp1a6eTSrJMQyNWG3dohVxsmtxuWk8m95Dv9h9L-AokTJvM |
| CitedBy_id | crossref_primary_10_1016_j_tig_2016_10_007 crossref_primary_10_1096_fj_14_259531 crossref_primary_10_1089_rej_2017_1930 crossref_primary_10_1155_2021_5586052 crossref_primary_10_1002_stem_635 crossref_primary_10_1134_S0006297920090047 crossref_primary_10_1016_j_stem_2010_02_008 crossref_primary_10_1089_scd_2016_0164 crossref_primary_10_1016_j_molmed_2016_06_005 crossref_primary_10_1002_jcb_23366 crossref_primary_10_1089_scd_2011_0127 crossref_primary_10_1155_2012_926147 crossref_primary_10_1177_2472555218775028 crossref_primary_10_1007_s10522_013_9455_2 crossref_primary_10_7554_eLife_47859 crossref_primary_10_1007_s11427_014_4666_6 crossref_primary_10_3390_genes9010039 crossref_primary_10_1016_j_bbrc_2023_149150 crossref_primary_10_1089_rej_2015_1763 crossref_primary_10_1038_s41598_018_24421_z crossref_primary_10_1016_j_expneurol_2013_07_010 crossref_primary_10_3233_JHD_160199 crossref_primary_10_1134_S106236042470005X crossref_primary_10_1002_jcp_24537 crossref_primary_10_1016_j_bbi_2024_04_023 crossref_primary_10_1016_j_wneu_2015_05_076 crossref_primary_10_2217_rme_12_64 crossref_primary_10_5504_BBEQ_2011_0103 crossref_primary_10_1007_s00441_017_2749_y crossref_primary_10_1242_dmm_029009 crossref_primary_10_1016_j_jocn_2021_09_042 crossref_primary_10_1093_hmg_ddu071 crossref_primary_10_1242_jcs_099192 crossref_primary_10_1016_j_bbrc_2020_02_092 crossref_primary_10_4236_ojrm_2013_23010 crossref_primary_10_1016_j_stemcr_2018_04_001 crossref_primary_10_1146_annurev_genet_120417_031534 crossref_primary_10_1161_CIRCRESAHA_116_304668 crossref_primary_10_1007_s11626_011_9478_4 crossref_primary_10_3390_ijms20051236 crossref_primary_10_1371_journal_pone_0017128 crossref_primary_10_1186_scrt99 crossref_primary_10_2217_rme_10_51 crossref_primary_10_3389_fcvm_2018_00004 crossref_primary_10_3727_096368912X655208 crossref_primary_10_3389_fcell_2022_1089970 crossref_primary_10_1016_j_mrfmmm_2011_05_008 crossref_primary_10_1083_jcb_201310135 crossref_primary_10_4061_2011_619583 crossref_primary_10_1016_j_stem_2015_05_004 crossref_primary_10_12729_jbtr_2017_18_1_007 crossref_primary_10_1038_mt_2010_269 crossref_primary_10_1089_scd_2013_0040 crossref_primary_10_1371_journal_pone_0013703 crossref_primary_10_1038_srep30013 crossref_primary_10_1155_2020_1061470 crossref_primary_10_1161_ATVBAHA_113_302462 crossref_primary_10_1016_j_yexcr_2017_07_002 crossref_primary_10_1038_s41467_024_48917_7 crossref_primary_10_3390_jpm11111050 crossref_primary_10_2144_000113493 crossref_primary_10_1007_s10522_015_9579_7 crossref_primary_10_1002_wdev_308 crossref_primary_10_1016_j_scr_2019_101540 crossref_primary_10_1016_j_blre_2011_06_004 crossref_primary_10_1016_j_ceb_2012_10_004 crossref_primary_10_2217_rme_10_21 crossref_primary_10_3389_fncel_2023_1003188 crossref_primary_10_1038_s43587_023_00539_2 crossref_primary_10_1080_15476286_2015_1134413 crossref_primary_10_1016_j_scr_2016_08_006 crossref_primary_10_1016_j_gde_2012_09_003 crossref_primary_10_1002_stem_3297 crossref_primary_10_1016_j_brainres_2015_11_013 crossref_primary_10_1002_jnr_25173 crossref_primary_10_1111_acel_12182 crossref_primary_10_1371_journal_pone_0035824 crossref_primary_10_1093_cvr_cvs253 crossref_primary_10_1016_j_arr_2016_02_007 crossref_primary_10_1016_j_tice_2013_07_003 crossref_primary_10_1007_s12015_021_10254_3 crossref_primary_10_1161_ATVBAHA_111_230938 crossref_primary_10_4161_epi_6_1_13390 crossref_primary_10_1038_cr_2011_201 crossref_primary_10_1038_cr_2011_120 crossref_primary_10_1101_gad_173922_111 crossref_primary_10_3390_jcm3041402 crossref_primary_10_1371_journal_pone_0074202 crossref_primary_10_1002_jbm_a_35996 |
| ContentType | Journal Article |
| Copyright | COPYRIGHT 2009 Public Library of Science 2009 Suhr et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. Suhr et al. 2009 |
| Copyright_xml | – notice: COPYRIGHT 2009 Public Library of Science – notice: 2009 Suhr et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: Suhr et al. 2009 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM IOV ISR 3V. 7QG 7QL 7QO 7RV 7SN 7SS 7T5 7TG 7TM 7U9 7X2 7X7 7XB 88E 8AO 8C1 8FD 8FE 8FG 8FH 8FI 8FJ 8FK ABJCF ABUWG AEUYN AFKRA ARAPS ATCPS AZQEC BBNVY BENPR BGLVJ BHPHI C1K CCPQU D1I DWQXO FR3 FYUFA GHDGH GNUQQ H94 HCIFZ K9. KB. KB0 KL. L6V LK8 M0K M0S M1P M7N M7P M7S NAPCQ P5Z P62 P64 PATMY PDBOC PHGZM PHGZT PIMPY PJZUB PKEHL PPXIY PQEST PQGLB PQQKQ PQUKI PRINS PTHSS PYCSY RC3 7X8 5PM DOA |
| DOI | 10.1371/journal.pone.0008124 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Gale In Context: Opposing Viewpoints Gale In Context: Science ProQuest Central (Corporate) Animal Behavior Abstracts Bacteriology Abstracts (Microbiology B) Biotechnology Research Abstracts Nursing & Allied Health Database Ecology Abstracts Entomology Abstracts (Full archive) Immunology Abstracts Meteorological & Geoastrophysical Abstracts Nucleic Acids Abstracts Virology and AIDS Abstracts Agricultural Science Collection Health & Medical Collection (ProQuest) ProQuest Central (purchase pre-March 2016) Medical Database (Alumni Edition) ProQuest Pharma Collection Public Health Database (ProQuest) Technology Research Database ProQuest SciTech Collection ProQuest Technology Collection ProQuest Natural Science Collection Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) Materials Science & Engineering Collection ProQuest Central (Alumni) ProQuest One Sustainability ProQuest Central UK/Ireland Advanced Technologies & Aerospace Collection Agricultural & Environmental Science Collection ProQuest Central Essentials Biological Science Collection ProQuest Central Technology Collection Natural Science Collection Environmental Sciences and Pollution Management ProQuest One ProQuest Materials Science Collection ProQuest Central Korea Engineering Research Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Central Student AIDS and Cancer Research Abstracts SciTech Premium Collection ProQuest Health & Medical Complete (Alumni) Materials Science Database Nursing & Allied Health Database (Alumni Edition) Meteorological & Geoastrophysical Abstracts - Academic ProQuest Engineering Collection ProQuest Biological Science Collection Agricultural Science Database ProQuest Health & Medical Collection Medical Database Algology Mycology and Protozoology Abstracts (Microbiology C) Biological Science Database (ProQuest) Engineering Database Nursing & Allied Health Premium Advanced Technologies & Aerospace Database ProQuest Advanced Technologies & Aerospace Collection Biotechnology and BioEngineering Abstracts Environmental Science Database Materials Science Collection ProQuest Central Premium ProQuest One Academic (New) Publicly Available Content Database ProQuest Health & Medical Research Collection ProQuest One Academic Middle East (New) ProQuest One Health & Nursing ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Applied & Life Sciences ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China Engineering Collection Environmental Science Collection Genetics Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) DOAJ Directory of Open Access Journals |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Agricultural Science Database Publicly Available Content Database ProQuest Central Student ProQuest Advanced Technologies & Aerospace Collection ProQuest Central Essentials Nucleic Acids Abstracts SciTech Premium Collection ProQuest Central China Environmental Sciences and Pollution Management ProQuest One Applied & Life Sciences ProQuest One Sustainability Health Research Premium Collection Meteorological & Geoastrophysical Abstracts Natural Science Collection Health & Medical Research Collection Biological Science Collection ProQuest Central (New) ProQuest Medical Library (Alumni) Engineering Collection Advanced Technologies & Aerospace Collection Engineering Database Virology and AIDS Abstracts ProQuest Biological Science Collection ProQuest One Academic Eastern Edition Agricultural Science Collection ProQuest Hospital Collection ProQuest Technology Collection Health Research Premium Collection (Alumni) Biological Science Database Ecology Abstracts ProQuest Hospital Collection (Alumni) Biotechnology and BioEngineering Abstracts Environmental Science Collection Entomology Abstracts Nursing & Allied Health Premium ProQuest Health & Medical Complete ProQuest One Academic UKI Edition Environmental Science Database ProQuest Nursing & Allied Health Source (Alumni) Engineering Research Database ProQuest One Academic Meteorological & Geoastrophysical Abstracts - Academic ProQuest One Academic (New) Technology Collection Technology Research Database ProQuest One Academic Middle East (New) Materials Science Collection ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest One Health & Nursing ProQuest Natural Science Collection ProQuest Pharma Collection ProQuest Central ProQuest Health & Medical Research Collection Genetics Abstracts ProQuest Engineering Collection Biotechnology Research Abstracts Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Bacteriology Abstracts (Microbiology B) Algology Mycology and Protozoology Abstracts (Microbiology C) Agricultural & Environmental Science Collection AIDS and Cancer Research Abstracts Materials Science Database ProQuest Materials Science Collection ProQuest Public Health ProQuest Nursing & Allied Health Source ProQuest SciTech Collection Advanced Technologies & Aerospace Database ProQuest Medical Library Animal Behavior Abstracts Materials Science & Engineering Collection Immunology Abstracts ProQuest Central (Alumni) MEDLINE - Academic |
| DatabaseTitleList | MEDLINE - Academic Nucleic Acids Abstracts Nucleic Acids Abstracts MEDLINE Agricultural Science Database Nucleic Acids Abstracts |
| Database_xml | – sequence: 1 dbid: DOA name: DOAJ Directory of Open Access Journals url: https://www.doaj.org/ sourceTypes: Open Website – sequence: 2 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 3 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 4 dbid: 8FG name: ProQuest Technology Collection url: https://search.proquest.com/technologycollection1 sourceTypes: Aggregation Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Sciences (General) Zoology |
| DocumentTitleAlternate | Human Telomere Reprogramming |
| EISSN | 1932-6203 |
| EndPage | e8124 |
| ExternalDocumentID | 1292200300 oai_doaj_org_article_baf56e43755043ce90b90881425110f4 PMC2780312 2898380471 A472783473 19956585 10_1371_journal_pone_0008124 |
| Genre | Research Support, Non-U.S. Gov't Journal Article |
| GeographicLocations | United States--US East Lansing Michigan Michigan |
| GeographicLocations_xml | – name: East Lansing Michigan – name: Michigan – name: United States--US |
| GroupedDBID | --- 123 29O 2WC 53G 5VS 7RV 7X2 7X7 7XC 88E 8AO 8C1 8CJ 8FE 8FG 8FH 8FI 8FJ A8Z AAFWJ AAUCC AAWOE AAYXX ABDBF ABIVO ABJCF ABUWG ACGFO ACIHN ACIWK ACPRK ACUHS ADBBV ADRAZ AEAQA AENEX AEUYN AFKRA AFPKN AFRAH AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS AOIJS APEBS ARAPS ATCPS BAWUL BBNVY BCNDV BENPR BGLVJ BHPHI BKEYQ BPHCQ BVXVI BWKFM CCPQU CITATION CS3 D1I D1J D1K DIK DU5 E3Z EAP EAS EBD EMOBN ESX EX3 F5P FPL FYUFA GROUPED_DOAJ GX1 HCIFZ HH5 HMCUK HYE IAO IEA IGS IHR IHW INH INR IOV IPY ISE ISR ITC K6- KB. KQ8 L6V LK5 LK8 M0K M1P M48 M7P M7R M7S M~E NAPCQ O5R O5S OK1 OVT P2P P62 PATMY PDBOC PHGZM PHGZT PIMPY PQQKQ PROAC PSQYO PTHSS PYCSY RNS RPM SV3 TR2 UKHRP WOQ WOW ~02 ~KM 3V. BBORY CGR CUY CVF ECM EIF NPM PMFND 7QG 7QL 7QO 7SN 7SS 7T5 7TG 7TM 7U9 7XB 8FD 8FK AZQEC C1K DWQXO FR3 GNUQQ H94 K9. KL. M7N P64 PJZUB PKEHL PPXIY PQEST PQGLB PQUKI PRINS RC3 7X8 5PM PUEGO AAPBV ABPTK N95 |
| ID | FETCH-LOGICAL-c6724-c32548c62fa2ae4b910dd859d70f8463ac7d7c187b8187a5f15d28a6076494f43 |
| IEDL.DBID | M48 |
| ISSN | 1932-6203 |
| IngestDate | Sun Jun 04 06:37:07 EDT 2023 Wed Aug 27 01:32:03 EDT 2025 Thu Aug 21 13:55:45 EDT 2025 Fri Jul 11 06:48:35 EDT 2025 Fri Jul 11 01:43:20 EDT 2025 Mon Jul 21 09:56:03 EDT 2025 Fri Jul 11 08:13:56 EDT 2025 Fri Jul 25 10:14:18 EDT 2025 Tue Jun 10 21:31:22 EDT 2025 Fri Jun 27 03:40:28 EDT 2025 Fri Jun 27 04:02:22 EDT 2025 Thu May 22 20:58:03 EDT 2025 Wed Feb 19 02:33:34 EST 2025 Thu Apr 24 23:06:21 EDT 2025 Tue Jul 01 03:13:44 EDT 2025 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 12 |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. Creative Commons Attribution License |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c6724-c32548c62fa2ae4b910dd859d70f8463ac7d7c187b8187a5f15d28a6076494f43 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 Conceived and designed the experiments: STS EAC JC. Performed the experiments: STS EAC RMR SM. Analyzed the data: STS EAC RMR KW PJR ZB. Contributed reagents/materials/analysis tools: STS EAC RMR KW. Wrote the paper: STS EAC JC. |
| OpenAccessLink | https://doaj.org/article/baf56e43755043ce90b90881425110f4 |
| PMID | 19956585 |
| PQID | 1292200300 |
| PQPubID | 23462 |
| PageCount | e8124 |
| ParticipantIDs | plos_journals_1292200300 doaj_primary_oai_doaj_org_article_baf56e43755043ce90b90881425110f4 pubmedcentral_primary_oai_pubmedcentral_nih_gov_2780312 proquest_miscellaneous_745903195 proquest_miscellaneous_733716558 proquest_miscellaneous_21475486 proquest_miscellaneous_21436073 proquest_journals_1292200300 gale_infotracacademiconefile_A472783473 gale_incontextgauss_ISR_A472783473 gale_incontextgauss_IOV_A472783473 gale_healthsolutions_A472783473 pubmed_primary_19956585 crossref_citationtrail_10_1371_journal_pone_0008124 crossref_primary_10_1371_journal_pone_0008124 |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 20091202 |
| PublicationDateYYYYMMDD | 2009-12-02 |
| PublicationDate_xml | – month: 12 year: 2009 text: 20091202 day: 2 |
| PublicationDecade | 2000 |
| PublicationPlace | United States |
| PublicationPlace_xml | – name: United States – name: San Francisco – name: San Francisco, USA |
| PublicationTitle | PloS one |
| PublicationTitleAlternate | PLoS One |
| PublicationYear | 2009 |
| Publisher | Public Library of Science Public Library of Science (PLoS) |
| Publisher_xml | – name: Public Library of Science – name: Public Library of Science (PLoS) |
| References | PLoS One. 2010;5(3) doi: 10.1371/annotation/c786d141-fd0f-45fd-80ec-96d80be620dc |
| References_xml | – reference: - PLoS One. 2010;5(3) doi: 10.1371/annotation/c786d141-fd0f-45fd-80ec-96d80be620dc |
| SSID | ssj0053866 |
| Score | 2.3007877 |
| Snippet | Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a potential source of... Background Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a... BACKGROUND:Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a... Background Human induced pluripotent stem cells (IPSCs) have enormous potential in the development of cellular models of human disease and represent a... |
| SourceID | plos doaj pubmedcentral proquest gale pubmed crossref |
| SourceType | Open Website Open Access Repository Aggregation Database Index Database Enrichment Source |
| StartPage | e8124 |
| SubjectTerms | Age Aged Aging Analysis Animal genetic engineering Animal sciences Autografts Cancer Cell culture Cell Differentiation Cell Line Cell lines Cellular Reprogramming - genetics Chromosomes Deoxyribonucleic acid Developmental Biology/Aging Developmental Biology/Cell Differentiation Developmental Biology/Embryology Developmental Biology/Stem Cells DNA Elongation Embryo cells Embryonic stem cells Embryos Explants Fibroblasts Fibroblasts - cytology Fibroblasts - metabolism Genetic Vectors - genetics Genomes Health aspects Heterogeneity Humans Induced Pluripotent Stem Cells - cytology Induced Pluripotent Stem Cells - metabolism Laboratories Lentivirus - genetics Male Microscopy, Phase-Contrast Phenotype Pluripotency Skin Somatic cells Stem cell transplantation Stem cells Telomerase Telomere - metabolism Telomeres Teratoma Teratoma - pathology Tissues Zoology |
| SummonAdditionalLinks | – databaseName: DOAJ Directory of Open Access Journals dbid: DOA link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwrV1Lb9QwELbQnrggyquBQi2EBBzSOo4fyYFDQVQFCZCgRb1ZjmNDpSVZNbsH_j0zjndpUGk5cF1_m2zmYc9sZr4h5Bn3oWlEIXIRypALBwlrpZ3M61C51koW2oDdyB8-qqMT8f5Unl4Y9YU1YSM98Ci4_cYGqbwotUSuLedr1mBpTiEwNmYhMoHCmbdOpsY9GLxYqdQoV-piP-llb9F3fi8eg1xMDqLI17_ZlWeLeT9cFnL-WTl54Sg6vE1upRiSHoy_fYvc8N0dspW8dKAvEpX0y7vk1bGf9z_8uaftOHl-oGcdjXP5KP5lP1AktYwVWnA7uuzpYr6CbaTHSPrnPXJy-Pb4zVGeBibkTmkucldCulc5xYPl1osGQoG2rWTdahYgziit0612RaUbOKa1laGQLa-sYlqJWgRR3iezDkS0Taj1hWMAqVhQAiC2BmQrA77Xq7R2GSnX0jMusYnjUIu5ia_INGQVozAMytwkmWck33xrMbJpXIN_jYrZYJELO34AFmKShZjrLCQju6hWMzaWbjzaHAgdx4zoMiNPIwL5MDosuPlmV8Ng3n36-g-gL58noOcJFHoQh7OpyQGeCXm2JshttLL1Yw8GAi-OlYKMZWRnbXmXL-9ulmEvQGuxne9Xg-EQ_IIyyysRGkxEZYT-BaFLUIaSsroCImSNzW8yIw9Gd_itTGyUhgw0I3riKBMNTle6s--R1RzkAhflD_-Hyh-RmzzN-WB8h8yW5yv_GILHZfMk7hO_AJr1a6Y priority: 102 providerName: Directory of Open Access Journals – databaseName: Health & Medical Collection (ProQuest) dbid: 7X7 link: http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV1Lb9QwELZgERIXRMujgUIthAQc0iaOHScHhEqhKkg8BC1a9RI5jl0qhWTZ7B7498w43pSgsnBdf8kqM56XPQ9CnjBjy5LHPOQ2sSHXELBmUoswt5mulIhsZbEa-f2H9OiEv5uKqT9w63xa5UonOkVdtRrPyPfALjFMpIqil7MfIU6NwttVP0LjKrmGrcswpUtOh4ALZDlNfblcIuM9z53dWduYXWcMGR-ZI9e1f9DNk1nddpc5nn_mT_5mkA5vkZvek6T7Pes3yBXTbJINL6sdfeYbSj_fJNdPW3d4fpu8ODZ1-93MDX3dT6Lv6HlD3UE-PTB13VFwyPuMLfhjumjpp3oJaqVFz_rnHXJy-Ob44Cj0AxRCnUrGQ51A-JfplFnFlOEluAZVlYm8kpEFvyNRWlZSx5kswWxLJWwsKpapNJIpz7nlyV0yaYBYW4QqE-sIIFlkUw4QlQOyEhbv-TIpdUCSFR0L7buL45CLunBXZhKijJ4sBVK_8NQPSDg8Neu7a_wD_wpZNGCxN7b7oZ2fFV7UwPxbkRqeSIHd2bTJoxKTuWKO0VRk4SU7yOCiLzQdJLzY59KNHZFJQB47BPbHaDAB50wtu654-_Hrf4C-fB6BnnqQbYEcWvmiB_gm7Ls1Qm7hflt9dldcbPiAbK_24OXLO8My6Aa88FGNaZddwcAZBmYmaxEStkgaEPoXhEyAGakQ2RoIFzkWw4mA3OsF44KZWDgNEWlA5EhkRhwcrzTn31yXc6ALvJTdX__xD8gN5id6RGybTBbzpXkIbuKifOR0wS9GxWbc priority: 102 providerName: ProQuest |
| Title | Telomere Dynamics in Human Cells Reprogrammed to Pluripotency |
| URI | https://www.ncbi.nlm.nih.gov/pubmed/19956585 https://www.proquest.com/docview/1292200300 https://www.proquest.com/docview/21436073 https://www.proquest.com/docview/21475486 https://www.proquest.com/docview/733716558 https://www.proquest.com/docview/745903195 https://pubmed.ncbi.nlm.nih.gov/PMC2780312 https://doaj.org/article/baf56e43755043ce90b90881425110f4 http://dx.doi.org/10.1371/journal.pone.0008124 |
| Volume | 4 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwjV3Nb9MwFLe27sIFMb4WNroIIQGHVPmw4-Qwoa20DKSNaayotyhx7DEpJF3TSuzC3857jhsI6jYuPsQ_J8p7fvZ79vsg5LUvVZZRjzpUBcqhAgzWiAvmxCoSecpclSuMRj45DY8n9POUTTfIqmarIWC91rTDelKTeTH4eX3zHgT-QFdt4N5q0GBWlXKgNzmfbpIt2Js4iuoJbe8VQLrD0ATQ3Tays0HpPP7tat2bFVW9ThX916Pyry1q_Ig8NLqlfdhMhm2yIcvHZNtIb22_NSmm3z0hBxeyqH7IubQ_NBXpa_uqtPWBvj2URVHboJg3nlvwOXtR2WfFEpaXCjXsm6dkMh5dDI8dU0jBESH3qSMCMAMjEfoq9VNJM1AR8jxicc5dBfpHkAqec-FFPIPtm6dMeSz3ozR0eUhjqmjwjPRKINEOsVPpCRcgkatCCpA0BmTOFN73RZwLiwQr6iXCZBnHYhdFoq_OOFgbDTESpHliaG4Rpx01a7Js3IM_Qsa0WMyRrR9U88vEiByoAYqFkgacYZY2IWM3Q6cuj6JV5Sp4yT6yNWkCTltJTw4p1-VHeGCRVxqBeTJKdMS5TJd1nXz68u0_QF_PO6A3BqQqIIdITfAD_BPm3-ogd3CWrX67TkAh89GD0HUtsreaeeu799tuWCPw4ictZbWsEx-UYmBmcCeCwxQJLWLfguABMCNkLLoDQlmMQXHMIs8bcfjDTAygBsvUIrwjKB0OdnvKq-862znQBV7qv7j_s7vkgW-qe7j-Hukt5kv5ElTGRdYnm3zKoY2GHrbjj32ydTQ6PTvv60OYvl4lsP01-g0MD3H4 |
| linkProvider | Scholars Portal |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9QwELbKIgQXRMujgcJaCAQc0iaOHSeHCpWWapc-QLCtVlxC4tilUkiWza5Q_xS_kZkkmxJUCpde11-8ysx4HvE8CHnGtEkS7nKbG8_YXEHAGkgl7NAEKo2FY1KD1cgHh_7giL8bi_ES-bmohcG0yoVOrBR1Wij8Rr4BdolhIpXjvJ58t3FqFN6uLkZo1GKxp89-QMhWbg53gL_PGdt9O9oe2M1UAVv5knFbeRATBcpnJmax5gnYyzQNRJhKx4Ax9mIlU6ncQCZgy2QsjCtSFsQ-BPw85IZ7sO81ch0Mr4MnSo7bAA90h-835XmedDcaaVifFLler4wv4x3zV00JaG1Bb5IV5UWO7p_5mr8ZwN075HbjudKtWtSWyZLOV8hyoxtK-rJpYP1qhdz4XFQf6--SzZHOim96qulOPfm-pKc5rS4O6LbOspJCAFBniMEf01lBP2RzUGMFevJn98jRlZD2PunlQKxVQmPtKgcggWN8DpA4BGQqDN4rBlIqi3gLOkaq6WaOQzWyqLqikxDV1GSJkPpRQ32L2O1Tk7qbxz_wb5BFLRZ7cVc_FNOTqDna4G4Y4WvuSYHd4JQOnQSTx1yO0ZtjYJM-MjiqC1tbjRJtcVmNOZGeRZ5WCOzHkWPCz0k8L8to-P74P0CfPnZALxqQKYAcKm6KLOCdsM9XB7mK8rZ47TI6P2AWWVvI4MXL_XYZdBFeMMW5LuZlxMD5BmZ6lyIkiIhvEfoXhPSAGb4QwSUQLkIsvhMWeVAfjHNmYqE2RMAWkZ0j0-FgdyU__Vp1VQe6wKbs4eUv3yc3B6OD_Wh_eLj3iNxizTQRh62R3mw614_BRZ0lTyq9QMmXq1ZEvwCAL6IJ |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1bb9MwFLZGEYgXxMZlgUEtBAIesiaOHScPExor1cpgTLBNFS8hcexRKUtK0wr1r_HrOE6cjKJReNlr_cVVzjk-l_hcEHpGpEoS6lKbKk_ZVEDAGnDB7FAFIo2Zo1Klq5E_HPr7J_TdiI3W0M-mFkanVTY6sVLUaSH0N_Ie2CWiE6kcp6dMWsRRf_B68t3WE6T0TWszTqMWkQO5-AHhW7kz7AOvnxMyeHu8t2-bCQO28DmhtvAgPgqET1RMYkkTsJ1pGrAw5Y4Cw-zFgqdcuAFPwK7xmCmXpSSIfQj-aUgV9WDfa-g695irzxgftcEe6BHfN6V6Hnd7RjK2J0UutytDTOiSKawmBrR2oTPJivIyp_fP3M3fjOHgDrptvFi8W4vdOlqT-QZaN3qixC9NM-tXG-jGl6L6cH8X7RzLrDiXU4n7izw-H4sSj3NcXSLgPZllJYZgoM4Wgz_GswIfZXNQaYX26hf30MmVkPY-6uRArE2EY-kKByCBo3wKkDgEZMqUvmMMOBcW8ho6RsJ0NtcDNrKouq7jEOHUZIk09SNDfQvZ7VOTurPHP_BvNItarO7LXf1QTM8ic8zB9VDMl9TjTHeGEzJ0Ep1I5lIdyTkKNulqBkd1kWurXaJdyquRJ9yz0NMKoXtz5FrKz-J5WUbDj6f_Afr8aQn0woBUAeQQsSm4gHfSPb-WkJta3prXLqOLw2ahrUYGL1_utsugl_RlU5zLYl5GBBxxYKa3EsFBRHwL4b8guAfM8BkLVkAoC3UhHrPQg_pgXDBTF21DNGwhvnRklji4vJKPv1Ud1oEusCl5uPrlu-gmqKDo_fDw4BG6RcxgEYdsoc5sOpePwVudJU8qtYDR16vWQ78ATyCmPw |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Telomere+Dynamics+in+Human+Cells+Reprogrammed+to+Pluripotency&rft.jtitle=PloS+one&rft.au=Suhr%2C+Steven+T&rft.au=Chang%2C+Eun+Ah&rft.au=Rodriguez%2C+Ramon+M&rft.au=Wang%2C+Kai&rft.date=2009-12-02&rft.eissn=1932-6203&rft.volume=4&rft.issue=2&rft_id=info:doi/10.1371%2Fjournal.pone.0008124&rft.externalDBID=NO_FULL_TEXT |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1932-6203&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1932-6203&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1932-6203&client=summon |