Antimicrobial and antibiofilm efficacy of a copper/calcium hydroxide-based endodontic paste against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans
Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intr...
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| Published in | Dental Materials Journal Vol. 38; no. 4; pp. 591 - 603 |
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| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japan
The Japanese Society for Dental Materials and Devices
26.07.2019
Japanese Society for Dental Materials and Devices Japan Science and Technology Agency |
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| Online Access | Get full text |
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| Abstract | Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intracanal irrigants, antimicrobial drugs and host immune responses. The aim of this study was to evaluate the in vitro efficacy of a Cu/CaOH2-based endodontic paste, against bacteria and fungi, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. We found that such compound significantly reduced microbial replication time and cell growth. Moreover, biofilm formation and persistence were also affected; treated biofilms showed both a reduced number of cells and levels of released pyoverdine. This study provides the first evidence on effectiveness of this endodontic compound against microbial biofilms. Given its wide range of action, its use in prevention and treatment of the main oral biofilm-associated infections will be discussed. |
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| AbstractList | Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intracanal irrigants, antimicrobial drugs and host immune responses. The aim of this study was to evaluate the in vitro efficacy of a Cu/CaOH2-based endodontic paste, against bacteria and fungi, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. We found that such compound significantly reduced microbial replication time and cell growth. Moreover, biofilm formation and persistence were also affected; treated biofilms showed both a reduced number of cells and levels of released pyoverdine. This study provides the first evidence on effectiveness of this endodontic compound against microbial biofilms. Given its wide range of action, its use in prevention and treatment of the main oral biofilm-associated infections will be discussed. Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intracanal irrigants, antimicrobial drugs and host immune responses. The aim of this study was to evaluate the in vitro efficacy of a Cu/CaOH -based endodontic paste, against bacteria and fungi, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. We found that such compound significantly reduced microbial replication time and cell growth. Moreover, biofilm formation and persistence were also affected; treated biofilms showed both a reduced number of cells and levels of released pyoverdine. This study provides the first evidence on effectiveness of this endodontic compound against microbial biofilms. Given its wide range of action, its use in prevention and treatment of the main oral biofilm-associated infections will be discussed. Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intracanal irrigants, antimicrobial drugs and host immune responses. The aim of this study was to evaluate the in vitro efficacy of a Cu/CaOH2-based endodontic paste, against bacteria and fungi, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. We found that such compound significantly reduced microbial replication time and cell growth. Moreover, biofilm formation and persistence were also affected; treated biofilms showed both a reduced number of cells and levels of released pyoverdine. This study provides the first evidence on effectiveness of this endodontic compound against microbial biofilms. Given its wide range of action, its use in prevention and treatment of the main oral biofilm-associated infections will be discussed.Endodontic biofilm is a microbial community, enclosed in a polymeric matrix of polysaccharide origin where are found pathogens, like bacteria and opportunistic fungi responsible for various endodontic pathologies. As clinical importance is the fact, that biofilm is extremely resistant to common intracanal irrigants, antimicrobial drugs and host immune responses. The aim of this study was to evaluate the in vitro efficacy of a Cu/CaOH2-based endodontic paste, against bacteria and fungi, such as Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. We found that such compound significantly reduced microbial replication time and cell growth. Moreover, biofilm formation and persistence were also affected; treated biofilms showed both a reduced number of cells and levels of released pyoverdine. This study provides the first evidence on effectiveness of this endodontic compound against microbial biofilms. Given its wide range of action, its use in prevention and treatment of the main oral biofilm-associated infections will be discussed. |
| Author | METO, Aida PEPPOLONI, Samuele COLOMBARI, Bruna PERICOLINI, Eva METO, Agron SALA, Arianna BLASI, Elisabetta |
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| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/31257304$$D View this record in MEDLINE/PubMed |
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| Copyright | 2019 The Japanese Society for Dental Materials and Devices Copyright Japan Science and Technology Agency 2019 |
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| CorporateAuthor | Aldent University Faculty of Dental Medicine School of Specialization in Microbiology and Virology Department of Surgical School of Doctorate in Clinical and Experimental Medicine Oncological and Regenerative Medicine; University of Modena and Reggio Emilia Medical Dental and Morphological Sciences with interest in Transplant University of Modena and Reggio Emilia Department of Therapy |
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Proc Natl Acad Sci USA 2005; 102: 11076-11081. 44) Du WL, Niu SS, Xu YL, Xu ZR, Fan CL. Antibacterial activity of chitosan tripolyphosphate nanoparticles loaded with various metal ions. Carbohydr Polym 2009; 75: 385-389. 17) Nair PN. On the causes of persistent apical periodontitis: a review. Int Endod J 2006; 39: 249-281. 23) Muto Y, Goto S. Transformation by extracellular DNA produced by Pseudomonas aeruginosa. Microbiol Immunol 1986; 30: 621-628. 45) Allesen-Holm M, Barken KB, Yang L, Klausen M, Webb JS, Kjelleberg S, et al. A characterization of DNA release in Pseudomonas aeruginosa cultures and biofilms. Mol Microbiol 2006; 59: 1114-1128. 46) Das T, Sharma PK, Busscher HJ, Van der Mei HC, Krom BP. Role of extracellular DNA in initial bacterial adhesion and surface aggregation. Appl Environ Microbiol 2010; 76: 3405-3408. 12) Arnold M, Ricucci D, Siqueira JF Jr. Infection in a complex network of apical ramifications as the cause of persistent apical periodontitis: a case report. J Endod 2013; 39: 1179-1184. 3) Siqueira JF Jr, Rôças IN, Ricucci D. Biofilms in endodontic infection. Endod Topics 2010; 22: 33-49. 22 44 23 45 24 46 25 26 27 28 29 30 31 10 32 11 33 12 34 13 35 14 36 15 37 16 38 17 39 18 19 1 2 3 4 5 6 7 8 9 40 41 20 42 21 43 |
| References_xml | – reference: 34) Haapasalo M, Qian W, Portenier I, Waltimo T. Effects of dentin on the antimicrobial properties of endodontic medicaments. J Endod 2007; 33: 917-925. – reference: 3) Siqueira JF Jr, Rôças IN, Ricucci D. Biofilms in endodontic infection. Endod Topics 2010; 22: 33-49. – reference: 18) Kim YS, Lee ES, Kwon HK, Kim BI. Monitoring the maturation process of a dental microcosm biofilm using the quantitative light-induced fluorescence-digital (QLF-D). Dent J 2014; 42: 691-696. – reference: 21) Cornelis P, Matthijs S. Diversity of siderophore-mediated iron uptake in fluorescent pseudomonas: not only pyoverdines. Environ Microbiol 2002; 4: 787-798. – reference: 46) Das T, Sharma PK, Busscher HJ, Van der Mei HC, Krom BP. Role of extracellular DNA in initial bacterial adhesion and surface aggregation. Appl Environ Microbiol 2010; 76: 3405-3408. – reference: 7) Carbajal Mejía JB. 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| Title | Antimicrobial and antibiofilm efficacy of a copper/calcium hydroxide-based endodontic paste against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans |
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