Role for protease activity in visceral pain in irritable bowel syndrome

• Published in: The Journal of clinical investigation Vol. 117; no. 3; pp. 636 - 647
• Main Authors: Cenac, Nicolas, Andrews, Christopher N., Holzhausen, Marinella, Chapman, Kevin, Cottrell, Graeme, Andrade-Gordon, Patricia, Steinhoff, Martin, Barbara, Giovanni, Beck, Paul, Bunnett, Nigel W., Sharkey, Keith A., Ferraz, Jose Geraldo P., Shaffer, Eldon, Vergnolle, Nathalie
• Format: Journal Article
• Language: English
• Published: United States American Society for Clinical Investigation 01.03.2007
• Subjects: Abdominal Pain - enzymology; Abdominal Pain - etiology; Adult; alpha 1-Antitrypsin - analysis; alpha 1-Antitrypsin - metabolism; Biomedical research; Biopsy; Colon; Colon - enzymology; Colon - innervation; Colon - pathology; Constipation; Diarrhea; Endopeptidases - analysis; Endopeptidases - drug effects; Endopeptidases - physiology; Female; Humans; Hyperalgesia; Hyperalgesia - enzymology; Hyperalgesia - etiology; Inflammatory bowel disease; Irritable bowel syndrome; Irritable Bowel Syndrome - complications; Irritable Bowel Syndrome - enzymology; Irritable Bowel Syndrome - pathology; Male; Middle Aged; Neurons, Afferent - drug effects; Neurons, Afferent - metabolism; NF-kappa B - metabolism; Patients; Physiological aspects; Proteases; Receptor, PAR-2 - agonists; Rectum; Serine Proteinase Inhibitors - pharmacology; Trypsin - analysis; Trypsin - drug effects; Trypsin - metabolism; Tryptases - analysis; Tryptases - antagonists & inhibitors; Tryptases - metabolism
• ISSN: 0021-9738; 1558-8238
• DOI: 10.1172/JCI29255

Mediators involved in the generation of symptoms in patients with irritable bowel syndrome (IBS) are poorly understood. Here we show that colonic biopsy samples from IBS patients release increased levels of proteolytic activity (arginine cleavage) compared to asymptomatic controls. This was dependen...