Analysis of 18 urinary mercapturic acids by two high-throughput multiplex-LC-MS/MS methods

• Published in: Analytical and bioanalytical chemistry Vol. 407; no. 18; pp. 5463 - 5476
• Main Authors: Pluym, Nikola, Gilch, Gerhard, Scherer, Gerhard, Scherer, Max
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2015; Springer; Springer Nature B.V
• Subjects: Acetylcysteine - analysis; Acetylcysteine - urine; Acids; acrolein; acrylamides; Acrylonitrile; Analysis; Analytical Chemistry; Benzene; Biochemistry; Biomarkers; Characterization and Evaluation of Materials; Chemical properties; Chemicals; Chemistry; Chemistry and Materials Science; Chromatography, Liquid - methods; clinical trials; Cost analysis; cost effectiveness; Ethylene; ethylene oxide; Food Science; glutathione; guidelines; High-Throughput Screening Assays - methods; Humans; Hydrocarbons; Immunoassays; Laboratory Medicine; Limit of Detection; Liquid chromatography; Mass spectrometry; Mathematical analysis; Metabolism; Metabolites; methods; Monitoring/Environmental Analysis; Multiplexing; Organic acids; propylene oxide; Research Paper; Salt; smoking (habit); Smoking - urine; Styrene; Styrenes; t-test; Tandem Mass Spectrometry - methods; Thiols; Tobacco smoke; Toluene; toxic substances; Toxicants; Toxins; Urinalysis - methods; Urine; Vinyl chloride; VOCs; Volatile organic compounds; Germany; Israel; Biomarkers; LC-MS/MS; Biomonitoring; Restricted access material (RAM); Mercapturic acid; Urinary metabolites
• ISSN: 1618-2642; 1618-2650
• DOI: 10.1007/s00216-015-8719-x

Mercapturic acids (MAs) are metabolic end products formed from conjugates between glutathione and electrophilic compounds. MAs are, therefore, suitable biomarkers of exposure to toxicants, which are either electrophiles by themselves or metabolized to electrophilic intermediates. We developed and va...