Analysis of allelic expression patterns in clonal somatic cells by single-cell RNA–seq

• Published in: Nature genetics Vol. 48; no. 11; pp. 1430 - 1435
• Main Authors: Reinius, Björn, Mold, Jeff E, Ramsköld, Daniel, Deng, Qiaolin, Johnsson, Per, Michaëlsson, Jakob, Frisén, Jonas, Sandberg, Rickard
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2016; Nature Publishing Group
• Subjects: 38/39; 38/91; 631/208/199; 631/208/212/2019; 631/208/514/1949; 64/60; Agriculture; Alleles; Animal Genetics and Genomics; Animals; Biomedicine; Cancer Research; CD8-Positive T-Lymphocytes - metabolism; Cell cycle; Cells, Cultured; Chromosomes; Clone Cells - metabolism; Cloning; Experiments; Female; Fibroblasts; Fibroblasts - metabolism; Gene expression; Gene Expression Profiling - methods; Gene Function; Genetic variation; Heterogeneity; Human Genetics; Humans; Identification and classification; letter; Lymphocytes; Male; Methods; Mice; Mice, Inbred C57BL; Mice, Inbred Strains; Observations; Polymorphism, Single Nucleotide; Properties; RNA sequencing; Sequence Analysis, RNA; Studies

Rickard Sandberg and colleagues use allele-sensitive single-cell RNA–seq on primary mouse fibroblasts and human T cells to study clonal and dynamic monoallelic expression patterns. They find that the majority of random monoallelic expression of autosomal genes occurs transiently within individual ce...