Constraints and advances in high-solids enzymatic hydrolysis of lignocellulosic biomass: a critical review

• Published in: Biotechnology for biofuels Vol. 13; no. 1; p. 58
• Main Authors: da Silva, Ayla Sant’Ana, Espinheira, Roberta Pereira, Teixeira, Ricardo Sposina Sobral, de Souza, Marcella Fernandes, Ferreira-Leitão, Viridiana, Bon, Elba P. S.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 23.03.2020; BioMed Central; BMC
• Subjects: adsorption; biofuels; Biomass; capital; Cellulases inhibition; Cellulolytic enzymes; Cellulose; Chemical properties; Chemicals; Conduction; Costs; economic feasibility; Enzymatic hydrolysis; Enzyme engineering; Enzymes; Ethanol; Fed-batch strategy; feedstocks; Formulations; Glucan; glucans; Glucose; Heat transfer; High solids loading; Hydrolysis; Impellers; Industrial production; Lignin; Lignocellulose; Lignocellulosic biomass; Materials; Methods; NMR; Nuclear magnetic resonance; Plant biomass; Pretreatment; Product inhibition; Production processes; Review; Rheological properties; Scientific papers; Solids; Sugar; sugars; Syrups; Water constraint; Brazil; Cellulosic ethanol; Biomass sugar syrups; Cellulases inhibition; Fed-batch strategy; Enzymatic hydrolysis; Water constraint; Biorefinery; Enzymatic hydrolysis reactors; Lignocellulosic biomass; High solids loading

The industrial production of sugar syrups from lignocellulosic materials requires the conduction of the enzymatic hydrolysis step at high-solids loadings (i.e., with over 15% solids [w/w] in the reaction mixture). Such conditions result in sugar syrups with increased concentrations and in improvemen...