Base editing: precision chemistry on the genome and transcriptome of living cells

• Published in: Nature reviews. Genetics Vol. 19; no. 12; pp. 770 - 788
• Main Authors: Rees, Holly A., Liu, David R.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.12.2018; Nature Publishing Group
• Subjects: 631/1647/1511; 631/1647/1513; 631/337/1645/1944; 631/337/4041/3196; 631/61/338; Agriculture; Animal Genetics and Genomics; Animals; Base sequence; Biomedical and Life Sciences; Biomedicine; Cancer Research; CRISPR; CRISPR-Cas Systems; Deoxyribonucleic acid; DNA; DNA binding proteins; DNA Breaks, Double-Stranded; DNA damage; DNA glycosylase; DNA sequencing; Double-stranded RNA; Editing; Editors; Endonucleases; Enzymes; Gene Editing - methods; Gene expression; Gene Function; Gene mutation; Genome editing; Genomes; Genomics; Human Genetics; Humans; Mutation; Nuclease; Nucleases; Nucleotide sequence; Review Article; Ribonucleic acid; RNA; RNA editing; Therapeutic applications; Transcription; Transcription (Genetics); Transcriptome

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA sequence or RNA transcript abundance. Base editing is a newer genome-editing approach that uses components from CRISPR systems togeth...