Isolation and characterization of a cDNA from soybean and its homolog from Escherichia coli, which both complement the light sensitivity of Escherichia coli hemH mutant strain VS101

• Published in: Genes & Genetic Systems Vol. 76; no. 5; pp. 327 - 334
• Main Authors: Kanjo, N. (Kyoto Univ. (Japan)), Nakahigashi, K, Oeda, K, Inokuchi, H
• Format: Journal Article
• Language: English
• Published: Japan The Genetics Society of Japan 01.10.2001; Japan Science and Technology Agency
• Subjects: Aminolevulinic Acid - analysis; Bacteriophages - genetics; DNA; DNA, Bacterial; DNA, Complementary - chemistry; DNA, Complementary - isolation & purification; ESCHERICHIA COLI; Escherichia coli - genetics; Escherichia coli - growth & development; Escherichia coli - virology; Ferrochelatase - genetics; Gene Deletion; Genetic Complementation Test; Glycine max; Glycine max - genetics; GLYCINE SOJA; Heme - biosynthesis; hemH gene; ISOLATION; Light; Lysogeny; MUTANTS; Mutation; Open Reading Frames; protoporphyrin IX; Protoporphyrins - biosynthesis; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Transformation, Genetic; YicL protein

Using Escherichia coli strain VS101, whose hemH gene encoding the ferro-chelatase is partially defective, we isolated and analyzed a clone (designated lWH-1) from a l phage library of soybean (Glycine max) cDNA, which exhibited weak complementation activity against the light sensitivity of VS101. In...