Two approaches for estimating the lower limit of quantitation (LLOQ) of microRNA levels assayed as exploratory biomarkers by RT-qPCR

• Published in: BMC biotechnology Vol. 18; no. 1; p. 6
• Main Authors: Wolfinger, Russell D., Beedanagari, Sudheer, Boitier, Eric, Chen, Tao, Couttet, Philippe, Ellinger-Ziegelbauer, Heidrun, Guillemain, Gregory, Mariet, Claire, Mouritzen, Peter, O’Lone, Raegan, Pine, P. Scott, Sharapova, Tatiana, Yan, Jian, Yuen, Peter S., Thompson, Karol L.
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 02.02.2018; BioMed Central; BMC
• Subjects: Absolute quantitation; Animals; Biological markers; biomarkers; Calibration; data collection; drugs; Gene expression; Genetic Markers; guidelines; Limit of Detection; logit analysis; Lower limit of quantitation; Methodology; microRNA; MicroRNAs - genetics; Polymerase chain reaction; Quantitative genetics; Quantitative PCR; quantitative polymerase chain reaction; Rats; reverse transcriptase polymerase chain reaction; Reverse Transcriptase Polymerase Chain Reaction - methods; Reverse Transcriptase Polymerase Chain Reaction - statistics & numerical data; reverse transcription; Transcription (Genetics); uncertainty; Workflow; Lower limit of quantitation; Absolute quantitation; microRNA; Quantitative PCR

Circulating microRNAs are undergoing exploratory use as safety biomarkers in drug development. Reverse transcription quantitative polymerase chain reaction (RT-qPCR) is one common approach used to quantitate levels of microRNAs in samples that includes the use of a standard curve of calibrators fit...