rpoB, a promising marker for analyzing the diversity of bacterial communities by amplicon sequencing

• Published in: BMC microbiology Vol. 19; no. 1; pp. 171 - 16
• Main Authors: Ogier, Jean-Claude, Pagès, Sylvie, Galan, Maxime, Barret, Matthieu, Gaudriault, Sophie
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 29.07.2019; BioMed Central; BMC
• Subjects: 16S rRNA gene; Analysis; Bacteria; Bacterial genetics; Biodiversity and Ecology; DNA sequencing; Ecosystems; Entomopathogenic nematodes; Environmental Sciences; Genes; Genetic aspects; Genetic markers; Metabarcoding; Methodology; Microbial colonies; Microbiota (Symbiotic organisms); Mock communities; RNA; Roundworms; rpoB; Technology; France; Metabarcoding; Mock communities; Entomopathogenic nematodes; rpoB; 16S rRNA gene; metabarcoding; mock communities; entomopathogenic nematodes

Microbiome composition is frequently studied by the amplification and high-throughput sequencing of specific molecular markers (metabarcoding). Various hypervariable regions of the 16S rRNA gene are classically used to estimate bacterial diversity, but other universal bacterial markers with a finer...