Detecting SARS-CoV-2 3CLpro expression and activity using a polyclonal antiserum and a luciferase-based biosensor - O'Brien, Amornrat; Chen, Da-Yuan; Hackbart, Matthew; Close, Brianna J.; O'Brien, Timothy E.; Saeed, Mohsan; Baker, Susan C. (Virology (New York, N.Y.), 2021)
Keywords: 3CLpro; Animals; antiserum; antiviral agents; Antiviral Agents - pharmacology; assays; Biosensing Techniques - methods; biosensors; Coronavirus 3C Proteases - antagonists & inhibitors; Coronavirus 3C Proteases - genetics; Coronavirus 3C Proteases - immunology; Coronavirus 3C Proteases - metabolism; Coronavirus infections; Cross Reactions; drug development; enzyme activity; Immune Sera - immunology; Infectious Disease; Luciferases - genetics; Luciferases - metabolism; Main protease; Mpro; nsp5; pathogenesis; pGlo biosensor; polyclonal antibodies; polyproteins; Protease inhibitors; Protease Inhibitors - pharmacology; proteinase inhibitors; proteinases; Rabbits; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; SARS-CoV-2; SARS-CoV-2 - drug effects; SARS-CoV-2 - genetics; SARS-CoV-2 - immunology; SARS-CoV-2 - metabolism; Severe acute respiratory syndrome coronavirus; Severe acute respiratory syndrome-related coronavirus - immunology; Severe acute respiratory syndrome-related coronavirus - metabolism; Viral Nonstructural Proteins - genetics; Viral Nonstructural Proteins - immunology; Viral Nonstructural Proteins - metabolism; virology; Virus Replication - drug effects; SARS-CoV-2; Main protease; Mpro; Protease inhibitors; pGlo biosensor; nsp5; 3CLpro