Multicolor two-photon imaging of in vivo cellular pathophysiology upon influenza virus infection using the two-photon IMPRESS

• Published in: Nature protocols Vol. 15; no. 3; pp. 1041 - 1065
• Main Authors: Ueki, Hiroshi, Wang, I-Hsuan, Zhao, Dongming, Gunzer, Matthias, Kawaoka, Yoshihiro
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01.03.2020; Nature Publishing Group
• Subjects: 631/1647/245/2186; 631/1647/767/1972; 631/250/2503; 631/326/596/1578; Analytical Chemistry; Animals; Antibodies; Biological Techniques; Biomedical and Life Sciences; Computational Biology/Bioinformatics; Development and progression; Dogs; Fluorescence microscopy; Fluorescent dyes; Fluorescent indicators; Gene Expression Regulation, Viral; Genes, Reporter; Host-virus relationships; Imaging; In vivo methods and tests; Inflammation; Influenza; Influenza A virus - genetics; Life Sciences; Luminescent Proteins - chemistry; Luminescent Proteins - metabolism; Lung - physiopathology; Lung - virology; Lung diseases; Lungs; Madin Darby Canine Kidney Cells; Medical research; Medicine, Experimental; Methods; Mice; Microarrays; Microscopy; Microscopy, Medical; Organic Chemistry; Orthomyxoviridae Infections - physiopathology; Orthomyxoviridae Infections - virology; Pathogenesis; Pathophysiology; Photons; Protocol; Respiratory diseases; Viruses; Japan

In vivo two-photon imaging is a valuable technique for studies of viral pathogenesis and host responses to infection in vivo. In this protocol, we describe a methodology for analyzing influenza virus–infected lung in vivo by two-photon imaging microscopy. We describe the surgical procedure, how to s...