Quantifying the phase separation property of chromatin-associated proteins under physiological conditions using an anti-1,6-hexanediol index

• Published in: Genome Biology Vol. 22; no. 1; p. 229
• Main Authors: Shi, Minglei, You, Kaiqiang, Chen, Taoyu, Hou, Chao, Liang, Zhengyu, Liu, Mingwei, Wang, Jifeng, Wei, Taotao, Qin, Jun, Chen, Yang, Zhang, Michael Q., Li, Tingting
• Format: Journal Article
• Language: English
• Published: England BioMed Central 17.08.2021; BMC
• Subjects: Biomolecular Condensates; Cell division; Chromatin; Chromatin - drug effects; Chromosomes; DNA-Binding Proteins - drug effects; Gene expression; genome; Genomes; Glycols - chemistry; Glycols - pharmacology; Humans; Hydrogenation; Hydrophobicity; Mass spectrometry; Methods; Physicochemical properties; Physiology; Proteins; Proteomics; quantitative analysis; RNA polymerase; Scientific imaging; separation; Sequence Analysis, Protein

Liquid-liquid phase separation (LLPS) is an important organizing principle for biomolecular condensation and chromosome compartmentalization. However, while many proteins have been reported to undergo LLPS, quantitative and global analysis of chromatin LLPS property remains absent. Here, by combinin...