Human antibody response to N-glycans present on plant-made influenza virus-like particle (VLP) vaccines

• Published in: Vaccine Vol. 32; no. 46; pp. 6098 - 6106
• Main Authors: Ward, Brian J., Landry, Nathalie, Trépanier, Sonia, Mercier, Geneviève, Dargis, Michèle, Couture, Manon, D’Aoust, Marc-André, Vézina, Louis-P.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 21.10.2014; Elsevier; Elsevier Limited
• Subjects: Adolescent; Adult; Allergens; Allergens - immunology; Allergies; Allergy; Allergy and Immunology; antennae; antibodies; Antibody Formation; Applied microbiology; avidin; Biological and medical sciences; Bromelain assay; bromelains; CCDs; Clinical trials; Cross Reactions - immunology; Cross-reactive carbohydrate determinants; Double-Blind Method; enzyme-linked immunosorbent assay; FDA approval; Food allergies; Fundamental and applied biological sciences. Psychology; Glycoproteins; Hemagglutination Inhibition Tests; Hemagglutinin Glycoproteins, Influenza Virus - immunology; hemagglutinins; Humans; Humoral response; Hypersensitivity; Hypersensitivity - immunology; IgE; IgG; Immunization; immunoglobulin E; Immunoglobulin E - blood; immunoglobulin G; Immunoglobulin G - blood; influenza; Influenza A virus; Influenza Vaccines - biosynthesis; Influenza Vaccines - immunology; mannose; Microbiology; Middle Aged; Miscellaneous; Nicotiana - metabolism; Nicotiana benthamiana; Plant-made vaccines; Plant-specific glycans; polysaccharides; Polysaccharides - immunology; Proteins; Rice; transfection; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Virus-Like Particle - biosynthesis; Vaccines, Virus-Like Particle - immunology; Virology; virus-like particle vaccines; Young Adult; Zea mays - immunology; USA, California; Indonesia; PBS; Allergy; Humoral response; MMXF3; CCD; IgE; Plant-made vaccines; IgG; MMF3; IgG/E; CCDs; Cross-reactive carbohydrate determinants; NA; Influenza A virus; Plant-specific glycans; Bromelain assay; MUXF3; HA; MMX; VLP; GlcNAc (Gn); hemagglutinin; virus-like particle; phosphate-buffered saline; MUXF 3; MMF 3; Manα1-6(Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc; N-acetylglucosamine; MMXF 3; (Manα1-3)(Manα1-6Xylβ1-2)Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc; (Manα1-3)(Manα1-6Xylβ1-2)Manβ1-4GlcNAcβ1-4GlcNAc; (Manα1-3)Manα1-6Manβ1-4GlcNAcβ1-4(Fucα1-3)GlcNAc; neuraminidase; immunoglobulin G or E; Orthomyxoviridae; Carbohydrate; Flulike syndrome; Humoral immunity; Human; Immunopathology; Immune response; Enzyme; Virus like particle; Antiinflammatory agent; Bromelains; Vaccine; Influenzavirus; Glycan; Virus; Influenzavirus A

Plant-made biotherapeutics are gathering momentum and some plant glycoproteins are allergens. Glycans with core β1-2xylose and α1,3fucose motifs and antennae terminated by mannose residues (e.g.: MMXF) are found on several plant allergens and can cross-react with glyco-epitopes from other sources. T...