Improvement of the cryopreservation method for the Babesia gibsoni parasite by using commercial freezing media

• Published in: Parasitology international Vol. 65; no. 5; pp. 532 - 535
• Main Authors: Kusakisako, Kodai, Masatani, Tatsunori, Yada, Yurika, Talactac, Melbourne Rio, Hernandez, Emmanuel Pacia, Maeda, Hiroki, Mochizuki, Masami, Tanaka, Tetsuya
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ireland Ltd 01.10.2016
• Subjects: Babesia bigemina; Babesia bovis; Babesia gibsoni; cattle; Cryopreservation; dimethyl sulfoxide; fetal bovine serum; freezing; Freezing medium; Gastroenterology and Hepatology; glycerol; in vitro culture; Infectious Disease; nitrogen; parasites; Plasmodium; polyvinylpyrrolidone; sodium chloride; sorbitol; Freezing medium; Cryopreservation; Babesia gibsoni

In vitro cultivation and cryopreservation under liquid nitrogen have already been reported and established for Babesia bovis and Babesia bigemina parasites. Although the in vitro cultivation methods for Babesia gibsoni have been reported and established, the cryopreservation methods for this parasit...