Controlled release of neurotrophin-3 from fibrin-based tissue engineering scaffolds enhances neural fiber sprouting following subacute spinal cord injury
This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-e...
Saved in:
| Published in | Biotechnology and bioengineering Vol. 104; no. 6; pp. 1207 - 1214 |
|---|---|
| Main Authors | , , |
| Format | Journal Article |
| Language | English |
| Published |
Hoboken
Wiley Subscription Services, Inc., A Wiley Company
15.12.2009
Wiley Wiley Subscription Services, Inc |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. Biotechnol. Bioeng. 2009; 104: 1207-1214. |
|---|---|
| AbstractList | Abstract
This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin‐3 (NT‐3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re‐exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin‐based delivery system with different concentrations of NT‐3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT‐3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT‐3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT‐3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. Biotechnol. Bioeng. 2009; 104: 1207–1214. © 2009 Wiley Periodicals, Inc. This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1000 ng/mL), or a fibrin scaffold containing 1000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin‐3 (NT‐3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re‐exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin‐based delivery system with different concentrations of NT‐3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT‐3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT‐3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT‐3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. Biotechnol. Bioeng. 2009; 104: 1207–1214. © 2009 Wiley Periodicals, Inc. This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. Biotechnol. Bioeng. 2009; 104: 1207-1214. This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate enhanced neural fiber sprouting. Long Evans rats received a T9 dorsal hemisection spinal cord injury. Two weeks later, the injury site was re-exposed, and either a fibrin scaffold alone, a fibrin scaffold containing a heparin-based delivery system with different concentrations of NT-3 (500 and 1,000 ng/mL), or a fibrin scaffold containing 1,000 ng/mL of NT-3 (no delivery system) was implanted into the injury site. The injured spinal cords were evaluated for morphological differences using markers for neurons, astrocytes, and chondroitin sulfate proteoglycans 2 weeks after treatment. The addition of 500 ng/mL of NT-3 with the delivery system resulted in an increase in neural fiber density compared to fibrin alone. These results demonstrate that the controlled release of NT-3 from fibrin scaffolds can enhance neural fiber sprouting even when treatment is delayed 2 weeks following injury. [PUBLICATION ABSTRACT] |
| Author | Parker, Stanley R Sakiyama-Elbert, Shelly E Johnson, Philip J |
| AuthorAffiliation | 2 Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA 3 Center for Materials Innovation, Washington University, St. Louis, MO 63130, USA 1 Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, USA |
| AuthorAffiliation_xml | – name: 2 Division of Plastic and Reconstructive Surgery, Department of Surgery, Washington University School of Medicine, St. Louis, MO 63110, USA – name: 3 Center for Materials Innovation, Washington University, St. Louis, MO 63130, USA – name: 1 Department of Biomedical Engineering, Washington University, St. Louis, MO 63130, USA |
| Author_xml | – sequence: 1 fullname: Johnson, Philip J – sequence: 2 fullname: Parker, Stanley R – sequence: 3 fullname: Sakiyama-Elbert, Shelly E |
| BackLink | http://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22174505$$DView record in Pascal Francis https://www.ncbi.nlm.nih.gov/pubmed/19603426$$D View this record in MEDLINE/PubMed |
| BookMark | eNqNkktvEzEQxy1URNPAgS8AKySEOGzrx65394IEEbSVAhxIxdHyeseJg2On9i4lH4Vvi_MgPCQkTqOZ-f1nxuM5QyfOO0DoMcHnBGN60Zr-nNKi4vfQiOCmyjFt8AkaYYx5zsqGnqKzGJfJrWrOH6BT0nDMCspH6PvEuz54a6HLAliQETKvMwdD8Cm-XhiXs0wHv8q0aUPy2oR0WW9iHCADNzcOIMXnWVRSa2-7mKIL6RTEXRlpt0oIWVwHP_RbMlHW3-00QyvV0ENKGpdI5UOXGbccwuYhuq-ljfDoYMfo5t3b2eQqn368vJ68nuaKc8Zz1bRQYl0qwrgkLccdqTtQShVE8qZhHWeVbiXjvORY17olFDhI1TBZNx1lbIxe7euuh3YFnYK0D2nFOpiVDBvhpRF_ZpxZiLn_KmhVY8Z4KvDiUCD42wFiL1YmKrBWOvBDFFXBcY0Jw_9BMl7WjDSJfPYXufRDSBuKghJWccyTGaOXe0gFH2MAfRyaYLE9DJEOQ-wOI7FPfn_lL_JwCQl4fgBk-kirQ_pBE48cpaQqSlwm7mLP3RkLm393FG-uZz9b53uFiT18Oypk-CJ4xapSfP5wKaaseD8hVzNBE_90z2vphZyHNMXNJ7pdIKkwLgrOfgDlke2R |
| CODEN | BIBIAU |
| CitedBy_id | crossref_primary_10_1002_adfm_201002468 crossref_primary_10_1002_jbm_a_33070 crossref_primary_10_1002_jbm_a_37596 crossref_primary_10_1016_j_msec_2017_02_102 crossref_primary_10_1039_c0jm04335d crossref_primary_10_1016_j_mtbio_2023_100668 crossref_primary_10_1016_j_neurol_2019_07_029 crossref_primary_10_1186_s13578_021_00694_2 crossref_primary_10_1093_rb_rbv012 crossref_primary_10_3389_fphar_2017_00245 crossref_primary_10_1016_j_biomaterials_2015_11_059 crossref_primary_10_1089_ten_tea_2011_0204 crossref_primary_10_1179_2045772313Y_0000000095 crossref_primary_10_1007_s00701_014_2089_6 crossref_primary_10_1016_j_biomaterials_2010_02_018 crossref_primary_10_1177_147323001204000423 crossref_primary_10_1007_s12015_023_10652_9 crossref_primary_10_1016_j_biomaterials_2018_02_013 crossref_primary_10_1016_j_mtbio_2021_100186 crossref_primary_10_1016_j_actbio_2020_09_057 crossref_primary_10_1016_j_matbio_2013_08_001 crossref_primary_10_2165_11631850_000000000_00000 crossref_primary_10_3892_etm_2013_1185 crossref_primary_10_1007_s12668_019_00682_4 crossref_primary_10_1089_ten_tea_2011_0097 crossref_primary_10_1155_2014_451639 crossref_primary_10_1002_adfm_201601521 crossref_primary_10_1016_j_actbio_2013_08_045 crossref_primary_10_1016_j_biomaterials_2023_122143 crossref_primary_10_1002_biot_201900275 crossref_primary_10_4061_2010_650857 crossref_primary_10_4103_1673_5374_290882 crossref_primary_10_1016_j_addr_2012_03_010 crossref_primary_10_4103_1673_5374_310696 crossref_primary_10_1177_0954411915611700 crossref_primary_10_1016_j_biomaterials_2009_11_084 crossref_primary_10_1089_ten_teb_2020_0267 crossref_primary_10_1021_acsbiomaterials_6b00167 crossref_primary_10_1088_1748_6041_7_1_012001 crossref_primary_10_1021_acs_langmuir_7b00909 crossref_primary_10_1039_C6IB00003G crossref_primary_10_1016_j_expneurol_2012_02_016 crossref_primary_10_1016_j_neulet_2012_02_025 crossref_primary_10_1016_j_biomaterials_2011_03_037 crossref_primary_10_1016_j_jconrel_2010_11_025 crossref_primary_10_1088_1748_605X_aaa4c3 crossref_primary_10_1016_j_fct_2014_05_019 crossref_primary_10_1016_j_jconrel_2011_06_035 crossref_primary_10_1093_rb_rbw004 crossref_primary_10_1098_rsif_2019_0505 crossref_primary_10_1186_1742_2094_8_183 crossref_primary_10_1016_j_brainres_2011_03_019 crossref_primary_10_1016_j_neulet_2012_01_058 crossref_primary_10_1155_2012_826754 crossref_primary_10_1038_s41598_017_06570_9 crossref_primary_10_1021_acs_bioconjchem_5b00397 crossref_primary_10_1186_s13287_021_02528_x crossref_primary_10_1002_bit_26074 crossref_primary_10_1016_j_jconrel_2014_05_040 crossref_primary_10_4103_1673_5374_156966 crossref_primary_10_1515_biol_2020_0028 crossref_primary_10_1016_j_mattod_2014_05_011 crossref_primary_10_1039_c0sm00173b crossref_primary_10_1007_s10439_011_0505_0 crossref_primary_10_1186_scrt480 crossref_primary_10_1088_1748_6041_7_2_024101 crossref_primary_10_1159_000446697 crossref_primary_10_1063_5_0035291 crossref_primary_10_1016_j_actbio_2019_12_033 crossref_primary_10_3727_096368910X524773 crossref_primary_10_12659_MSM_900265 crossref_primary_10_1016_j_biomaterials_2017_05_008 crossref_primary_10_1016_j_coche_2016_11_004 crossref_primary_10_1146_annurev_bioeng_121813_120655 crossref_primary_10_1002_jbm_a_36962 crossref_primary_10_1088_1748_6041_8_2_022001 |
| Cites_doi | 10.1006/exnr.2000.7413 10.1016/S0142-9612(03)00503-9 10.1002/jbm.a32343 10.1016/j.expneurol.2006.04.020 10.1016/j.jconrel.2004.05.003 10.1016/0006-8993(91)90506-Q 10.1002/jnr.20662 10.1016/j.jconrel.2006.07.005 10.1016/S0306-4522(02)00384-6 10.1089/neu.2006.23.617 10.1016/S0014-4886(03)00398-4 10.1038/367170a0 10.1016/j.expneurol.2006.07.030 10.1016/S0361-9230(99)00072-6 10.1523/JNEUROSCI.17-14-05560.1997 10.1089/neu.2006.23.263 10.1006/exnr.1997.6704 10.1002/cne.10381 10.1006/exnr.1998.6912 10.1006/exnr.2002.7980 10.1016/S0014-4886(03)00394-7 10.1016/j.biomaterials.2005.07.008 10.1016/j.jconrel.2006.05.005 10.1016/S0140-6736(02)07603-1 10.1523/JNEUROSCI.21-23-09334.2001 10.1016/S0168-3659(99)00221-7 |
| ContentType | Journal Article |
| Copyright | Copyright © 2009 Wiley Periodicals, Inc. 2015 INIST-CNRS 2009 Wiley Periodicals, Inc. Copyright John Wiley and Sons, Limited Dec 15, 2009 |
| Copyright_xml | – notice: Copyright © 2009 Wiley Periodicals, Inc. – notice: 2015 INIST-CNRS – notice: 2009 Wiley Periodicals, Inc. – notice: Copyright John Wiley and Sons, Limited Dec 15, 2009 |
| DBID | FBQ BSCLL IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QF 7QO 7QQ 7SC 7SE 7SP 7SR 7T7 7TA 7TB 7U5 8BQ 8FD C1K F28 FR3 H8D H8G JG9 JQ2 KR7 L7M L~C L~D P64 7TK 5PM |
| DOI | 10.1002/bit.22476 |
| DatabaseName | AGRIS Istex Pascal-Francis Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Aluminium Industry Abstracts Biotechnology Research Abstracts Ceramic Abstracts Computer and Information Systems Abstracts Corrosion Abstracts Electronics & Communications Abstracts Engineered Materials Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Materials Business File Mechanical & Transportation Engineering Abstracts Solid State and Superconductivity Abstracts METADEX Technology Research Database Environmental Sciences and Pollution Management ANTE: Abstracts in New Technology & Engineering Engineering Research Database Aerospace Database Copper Technical Reference Library Materials Research Database ProQuest Computer Science Collection Civil Engineering Abstracts Advanced Technologies Database with Aerospace Computer and Information Systems Abstracts Academic Computer and Information Systems Abstracts Professional Biotechnology and BioEngineering Abstracts Neurosciences Abstracts PubMed Central (Full Participant titles) |
| DatabaseTitle | MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Materials Research Database Technology Research Database Computer and Information Systems Abstracts – Academic Mechanical & Transportation Engineering Abstracts ProQuest Computer Science Collection Computer and Information Systems Abstracts Materials Business File Environmental Sciences and Pollution Management Aerospace Database Copper Technical Reference Library Engineered Materials Abstracts Biotechnology Research Abstracts Industrial and Applied Microbiology Abstracts (Microbiology A) Advanced Technologies Database with Aerospace ANTE: Abstracts in New Technology & Engineering Civil Engineering Abstracts Aluminium Industry Abstracts Electronics & Communications Abstracts Ceramic Abstracts METADEX Biotechnology and BioEngineering Abstracts Computer and Information Systems Abstracts Professional Solid State and Superconductivity Abstracts Engineering Research Database Corrosion Abstracts Neurosciences Abstracts |
| DatabaseTitleList | CrossRef Solid State and Superconductivity Abstracts MEDLINE Materials Research Database Engineering Research Database |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database – sequence: 3 dbid: FBQ name: AGRIS url: http://www.fao.org/agris/Centre.asp?Menu_1ID=DB&Menu_2ID=DB1&Language=EN&Content=http://www.fao.org/agris/search?Language=EN sourceTypes: Publisher |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Engineering Chemistry Biology Anatomy & Physiology |
| EISSN | 1097-0290 |
| EndPage | 1214 |
| ExternalDocumentID | 1912115521 10_1002_bit_22476 19603426 22174505 BIT22476 ark_67375_WNG_L34MC1HT_2 US201301700446 |
| Genre | article Journal Article Research Support, N.I.H., Extramural Feature |
| GrantInformation_xml | – fundername: NIH‐NINDS funderid: R01 NS051454 – fundername: NINDS NIH HHS grantid: R01 NS051454-04 – fundername: NINDS NIH HHS grantid: R01 NS051454 |
| GroupedDBID | --- -~X .3N .GA .GJ .Y3 05W 0R~ 10A 1L6 1OB 1OC 1ZS 23N 31~ 33P 3EH 3SF 3WU 4.4 4ZD 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52U 52W 52X 53G 5GY 5RE 5VS 66C 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAESR AAEVG AAHHS AAJUZ AANLZ AAONW AASGY AAXRX AAZKR ABCQN ABCUV ABCVL ABEML ABHUG ABIJN ABJNI ABPVW ACAHQ ACBWZ ACCFJ ACCZN ACGFO ACGFS ACIWK ACPOU ACPRK ACSCC ACSMX ACXBN ACXME ACXQS ADAWD ADBBV ADDAD ADEOM ADIZJ ADKYN ADMGS ADOZA ADXAS ADZMN ADZOD AEEZP AEIGN AEIMD AENEX AEQDE AEUQT AEUYR AFBPY AFFNX AFFPM AFGKR AFPWT AFRAH AFVGU AFZJQ AGJLS AI. AIAGR AIURR AIWBW AJBDE AJXKR ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB ASPBG ATUGU AUFTA AVWKF AZBYB AZFZN AZVAB BAFTC BDRZF BFHJK BHBCM BLYAC BMNLL BMXJE BNHUX BROTX BRXPI BY8 CS3 D-E D-F DCZOG DPXWK DR1 DR2 DRFUL DRSTM DU5 EBD EBS EJD EMOBN F00 F01 F04 F5P FBQ FEDTE G-S G.N GNP GODZA H.T H.X HBH HF~ HHY HHZ HVGLF HZ~ IX1 J0M JPC KQQ LATKE LAW LC2 LC3 LEEKS LH4 LH6 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MEWTI MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NDZJH NF~ NNB O66 O9- P2P P2W P2X P4D PALCI PQQKQ Q.N Q11 QB0 QRW R.K RBB RIWAO RJQFR RNS ROL RWI RX1 RYL SAMSI SUPJJ SV3 TN5 UB1 V2E VH1 W8V W99 WBKPD WH7 WIB WIH WIK WJL WNSPC WOHZO WQJ WRC WSB WXSBR WYISQ XFK XG1 XPP XSW XV2 Y6R ZGI ZXP ZZTAW ~02 ~IA ~KM ~WT AHBTC AITYG BSCLL HGLYW OIG 08R AAPBV IQODW CGR CUY CVF ECM EIF NPM AAYXX CITATION 7QF 7QO 7QQ 7SC 7SE 7SP 7SR 7T7 7TA 7TB 7U5 8BQ 8FD C1K F28 FR3 H8D H8G JG9 JQ2 KR7 L7M L~C L~D P64 7TK 5PM |
| ID | FETCH-LOGICAL-c6636-c9be50f5c136a1b60d18deccc41a6993d637fba366560f8fb12e6eac93a89d233 |
| IEDL.DBID | DR2 |
| ISSN | 0006-3592 |
| IngestDate | Tue Sep 17 21:24:58 EDT 2024 Fri Aug 16 23:39:28 EDT 2024 Sat Aug 17 03:14:04 EDT 2024 Thu Oct 10 18:29:00 EDT 2024 Fri Aug 23 03:03:36 EDT 2024 Sat Sep 28 07:57:02 EDT 2024 Sun Oct 22 16:03:23 EDT 2023 Sat Aug 24 01:15:28 EDT 2024 Wed Oct 30 09:54:54 EDT 2024 Wed Dec 27 19:16:50 EST 2023 |
| IsDoiOpenAccess | false |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Keywords | Neurotrophin Delivery system growth factors Tissue engineering Fiber Nerve Fibrin Regeneration Spinal cord trauma Biomaterial nerve regeneration Release Growth factor |
| Language | English |
| License | CC BY 4.0 2009 Wiley Periodicals, Inc. |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c6636-c9be50f5c136a1b60d18deccc41a6993d637fba366560f8fb12e6eac93a89d233 |
| Notes | http://dx.doi.org/10.1002/bit.22476 ArticleID:BIT22476 No benefit of any kind will be received either directly or indirectly by the authors. NIH-NINDS - No. R01 NS051454 istex:46734A4579D5368CE3627CB103A99C68F74DAF3D ark:/67375/WNG-L34MC1HT-2 ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
| OpenAccessLink | https://onlinelibrary.wiley.com/doi/pdfdirect/10.1002/bit.22476 |
| PMID | 19603426 |
| PQID | 213760621 |
| PQPubID | 48814 |
| PageCount | 8 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_2780336 proquest_miscellaneous_746080130 proquest_miscellaneous_743658319 proquest_journals_213760621 crossref_primary_10_1002_bit_22476 pubmed_primary_19603426 pascalfrancis_primary_22174505 wiley_primary_10_1002_bit_22476_BIT22476 istex_primary_ark_67375_WNG_L34MC1HT_2 fao_agris_US201301700446 |
| PublicationCentury | 2000 |
| PublicationDate | 15 December 2009 |
| PublicationDateYYYYMMDD | 2009-12-15 |
| PublicationDate_xml | – month: 12 year: 2009 text: 15 December 2009 day: 15 |
| PublicationDecade | 2000 |
| PublicationPlace | Hoboken |
| PublicationPlace_xml | – name: Hoboken – name: Hoboken, NJ – name: United States – name: New York |
| PublicationTitle | Biotechnology and bioengineering |
| PublicationTitleAlternate | Biotechnol. Bioeng |
| PublicationYear | 2009 |
| Publisher | Wiley Subscription Services, Inc., A Wiley Company Wiley Wiley Subscription Services, Inc |
| Publisher_xml | – name: Wiley Subscription Services, Inc., A Wiley Company – name: Wiley – name: Wiley Subscription Services, Inc |
| References | Prang P, Muller R, Eljaouhari A, Heckmann K, Kunz W, Weber T, Faber C, Vroemen M, Bogdahn U, Weidner N. 2006. The promotion of oriented axonal regrowth in the injured spinal cord by alginate-based anisotropic capillary hydrogels. Biomaterials 27(19): 3560-3569. Novikova LN, Novikov LN, Kellerth JO. 2002. Differential effects of neurotrophins on neuronal survival and axonal regeneration after spinal cord injury in adult rats. J Comp Neurol 452(3): 255-263. Sakiyama-Elbert SE, Hubbell JA. 2000. Development of fibrin derivatives for controlled release of heparin-binding growth factors. J Control Release 65(3): 389-402. Taylor SJ, Rosenzweig ES, McDonald JW III, Sakiyama-Elbert SE. 2006. Delivery of neurotrophin-3 from fibrin enhances neuronal fiber sprouting after spinal cord injury. J Control Release 113(3): 226-235. Lu P, Jones LL, Tuszynski MH. 2007. Axon regeneration through scars and into sites of chronic spinal cord injury. Exp Neurol 203(1): 8-21. Xu B, Michalski B, Racine RJ, Fahnestock M. 2002. Continuous infusion of neurotrophin-3 triggers sprouting, decreases the levels of TrkA and TrkC, and inhibits epileptogenesis and activity-dependent axonal growth in adult rats. Neuroscience 115(4): 1295-1308. Grill R, Murai K, Blesch A, Gage FH, Tuszynski MH. 1997a. Cellular delivery of neurotrophin-3 promotes corticospinal axonal growth and partial functional recovery after spinal cord injury. J Neurosci 17(14): 5560-5572. Jain A, Kim YT, McKeon RJ, Bellamkonda RV. 2006. In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury. Biomaterials 27(3): 497-504. Jin Y, Fischer I, Tessler A, Houle JD. 2002. Transplants of fibroblasts genetically modified to express BDNF promote axonal regeneration from supraspinal neurons following chronic spinal cord injury. Exp Neurol 177(1): 265-275. Tobias CA, Shumsky JS, Shibata M, Tuszynski MH, Fischer I, Tessler A, Murray M. 2003. Delayed grafting of BDNF and NT-3 producing fibroblasts into the injured spinal cord stimulates sprouting, partially rescues axotomized red nucleus neurons from loss and atrophy, and provides limited regeneration. Exp Neurol 184(1): 97-113. Hsu JY, Xu XM. 2005. Early profiles of axonal growth and astroglial response after spinal cord hemisection and implantation of Schwann cell-seeded guidance channels in adult rats. J Neurosci Res 82(4): 472-483. Taylor SJ, McDonald JW III, Sakiyama-Elbert SE. 2004. Controlled release of neurotrophin-3 from fibrin gels for spinal cord injury. J Control Release 98(2): 281-294. Johnson PJ, Parker SR, Sakiyama-Elbert SE. 2009. Fibrin-based tissue engineering scaffolds enhance neural fiber sprouting and delay the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury. J Biomed Mater Res A. DOI: 10.1002/jbm.a32343 Patist CM, Mulder MB, Gautier SE, Maquet V, Jerome R, Oudega M. 2004. Freeze-dried poly(D,L-lactic acid) macroporous guidance scaffolds impregnated with brain-derived neurotrophic factor in the transected adult rat thoracic spinal cord. Biomaterials 25(9): 1569-1582. Coumans JV, Lin TT, Dai HN, MacArthur L, McAtee M, Nash C, Bregman BS. 2001. Axonal regeneration and functional recovery after complete spinal cord transection in rats by delayed treatment with transplants and neurotrophins. J Neurosci 21(23): 9334-9344. Blits B, Dijkhuizen PA, Boer GJ, Verhaagen J. 2000. Intercostal nerve implants transduced with an adenoviral vector encoding neurotrophin-3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function. Exp Neurol 164(1): 25-37. Fawcett JW, Asher RA. 1999. The glial scar and central nervous system repair. Brain Res Bull 49(6): 377-391. McDonald JW, Sadowsky C. 2002. Spinal-cord injury. Lancet 359(9304): 417-425. Taylor SJ, Sakiyama-Elbert SE. 2006. Effect of controlled delivery of neurotrophin-3 from fibrin on spinal cord injury in a long term model. J Control Release 116(2): 204-210. Hagg T, Oudega M. 2006. Degenerative and spontaneous regenerative processes after spinal cord injury. J Neurotrauma 23(3-4): 264-280. Shumsky JS, Tobias CA, Tumolo M, Long WD, Giszter SF, Murray M. 2003. Delayed transplantation of fibroblasts genetically modified to secrete BDNF and NT-3 into a spinal cord injury site is associated with limited recovery of function. Exp Neurol 184(1): 114-130. Schnell L, Schneider R, Kolbeck R, Barde YA, Schwab ME. 1994. Neurotrophin-3 enhances sprouting of corticospinal tract during development and after adult spinal cord lesion. Nature 367(6459): 170-173. von Meyenburg J, Brosamle C, Metz GA, Schwab ME. 1998. Regeneration and sprouting of chronically injured corticospinal tract fibers in adult rats promoted by NT-3 and the mAb IN-1, which neutralizes myelin-associated neurite growth inhibitors. Exp Neurol 154(2): 583-594. Knoops B, Hubert I, Hauw JJ, van den Bosch de Aguilar P. 1991. Axonal growth and glial migration from co-cultured hippocampal and septal slices into fibrin-fibronectin-containing matrix of peripheral regeneration chambers: A light and electron microscope study. Brain Res 540(1-2): 183-194. Piantino J, Burdick JA, Goldberg D, Langer R, Benowitz LI. 2006. An injectable, biodegradable hydrogel for trophic factor delivery enhances axonal rewiring and improves performance after spinal cord injury. Exp Neurol 201(2): 359-367. Grill RJ, Blesch A, Tuszynski MH. 1997b. Robust growth of chronically injured spinal cord axons induced by grafts of genetically modified NGF-secreting cells. Exp Neurol 148(2): 444-452. Lynskey JV, Sandhu FA, Dai HN, McAtee M, Slotkin JR, MacArthur L, Bregman BS. 2006. Delayed intervention with transplants and neurotrophic factors supports recovery of forelimb function after cervical spinal cord injury in adult rats. J Neurotrauma 23(5): 617-634. 2004; 98 2007; 203 1994; 367 1991; 540 2006; 23 1997a; 17 2006; 27 2004; 25 2000; 65 1999; 49 2002; 177 2002; 452 2009 2002; 359 2003; 184 2002; 115 2000; 164 2005; 82 2006; 116 1998; 154 1997b; 148 2001; 21 2006; 201 2006; 113 16764857 - Exp Neurol. 2006 Oct;201(2):359-67 9204937 - J Neurosci. 1997 Jul 15;17(14):5560-72 11717367 - J Neurosci. 2001 Dec 1;21(23):9334-44 12453498 - Neuroscience. 2002;115(4):1295-308 16240391 - J Neurosci Res. 2005 Nov 15;82(4):472-83 10699297 - J Control Release. 2000 Apr 3;65(3):389-402 12353221 - J Comp Neurol. 2002 Oct 21;452(3):255-63 8114912 - Nature. 1994 Jan 13;367(6459):170-3 16629615 - J Neurotrauma. 2006 Mar-Apr;23(3-4):264-80 16797770 - J Control Release. 2006 Jul 20;113(3):226-35 15262419 - J Control Release. 2004 Aug 11;98(2):281-94 11844532 - Lancet. 2002 Feb 2;359(9304):417-25 14697859 - Biomaterials. 2004 Apr;25(9):1569-82 19165795 - J Biomed Mater Res A. 2010 Jan;92(1):152-63 17014846 - Exp Neurol. 2007 Jan;203(1):8-21 14637085 - Exp Neurol. 2003 Nov;184(1):114-30 12429228 - Exp Neurol. 2002 Sep;177(1):265-75 16689666 - J Neurotrauma. 2006 May;23(5):617-34 16500703 - Biomaterials. 2006 Jul;27(19):3560-9 16919351 - J Control Release. 2006 Nov 28;116(2):204-10 2054610 - Brain Res. 1991 Feb 1;540(1-2):183-94 16099038 - Biomaterials. 2006 Jan;27(3):497-504 14637084 - Exp Neurol. 2003 Nov;184(1):97-113 9417824 - Exp Neurol. 1997 Dec;148(2):444-52 9878193 - Exp Neurol. 1998 Dec;154(2):583-94 10483914 - Brain Res Bull. 1999 Aug;49(6):377-91 10877912 - Exp Neurol. 2000 Jul;164(1):25-37 e_1_2_1_20_1 e_1_2_1_23_1 e_1_2_1_24_1 e_1_2_1_21_1 e_1_2_1_22_1 e_1_2_1_27_1 e_1_2_1_28_1 e_1_2_1_25_1 e_1_2_1_26_1 e_1_2_1_7_1 e_1_2_1_8_1 e_1_2_1_5_1 e_1_2_1_6_1 e_1_2_1_3_1 e_1_2_1_12_1 e_1_2_1_4_1 e_1_2_1_13_1 e_1_2_1_10_1 e_1_2_1_2_1 e_1_2_1_11_1 e_1_2_1_16_1 e_1_2_1_17_1 Prang P (e_1_2_1_19_1) 2006; 27 e_1_2_1_14_1 e_1_2_1_15_1 e_1_2_1_9_1 e_1_2_1_18_1 |
| References_xml | – volume: 65 start-page: 389 issue: 3 year: 2000 end-page: 402 article-title: Development of fibrin derivatives for controlled release of heparin‐binding growth factors publication-title: J Control Release – volume: 367 start-page: 170 issue: 6459 year: 1994 end-page: 173 article-title: Neurotrophin‐3 enhances sprouting of corticospinal tract during development and after adult spinal cord lesion publication-title: Nature – volume: 203 start-page: 8 issue: 1 year: 2007 end-page: 21 article-title: Axon regeneration through scars and into sites of chronic spinal cord injury publication-title: Exp Neurol – volume: 49 start-page: 377 issue: 6 year: 1999 end-page: 391 article-title: The glial scar and central nervous system repair publication-title: Brain Res Bull – volume: 21 start-page: 9334 issue: 23 year: 2001 end-page: 9344 article-title: Axonal regeneration and functional recovery after complete spinal cord transection in rats by delayed treatment with transplants and neurotrophins publication-title: J Neurosci – volume: 113 start-page: 226 issue: 3 year: 2006 end-page: 235 article-title: Delivery of neurotrophin‐3 from fibrin enhances neuronal fiber sprouting after spinal cord injury publication-title: J Control Release – volume: 154 start-page: 583 issue: 2 year: 1998 end-page: 594 article-title: Regeneration and sprouting of chronically injured corticospinal tract fibers in adult rats promoted by NT‐3 and the mAb IN‐1, which neutralizes myelin‐associated neurite growth inhibitors publication-title: Exp Neurol – volume: 164 start-page: 25 issue: 1 year: 2000 end-page: 37 article-title: Intercostal nerve implants transduced with an adenoviral vector encoding neurotrophin‐3 promote regrowth of injured rat corticospinal tract fibers and improve hindlimb function publication-title: Exp Neurol – volume: 184 start-page: 97 issue: 1 year: 2003 end-page: 113 article-title: Delayed grafting of BDNF and NT‐3 producing fibroblasts into the injured spinal cord stimulates sprouting, partially rescues axotomized red nucleus neurons from loss and atrophy, and provides limited regeneration publication-title: Exp Neurol – volume: 82 start-page: 472 issue: 4 year: 2005 end-page: 483 article-title: Early profiles of axonal growth and astroglial response after spinal cord hemisection and implantation of Schwann cell‐seeded guidance channels in adult rats publication-title: J Neurosci Res – volume: 115 start-page: 1295 issue: 4 year: 2002 end-page: 1308 article-title: Continuous infusion of neurotrophin‐3 triggers sprouting, decreases the levels of TrkA and TrkC, and inhibits epileptogenesis and activity‐dependent axonal growth in adult rats publication-title: Neuroscience – volume: 184 start-page: 114 issue: 1 year: 2003 end-page: 130 article-title: Delayed transplantation of fibroblasts genetically modified to secrete BDNF and NT‐3 into a spinal cord injury site is associated with limited recovery of function publication-title: Exp Neurol – volume: 27 start-page: 497 issue: 3 year: 2006 end-page: 504 article-title: In situ gelling hydrogels for conformal repair of spinal cord defects, and local delivery of BDNF after spinal cord injury publication-title: Biomaterials – volume: 177 start-page: 265 issue: 1 year: 2002 end-page: 275 article-title: Transplants of fibroblasts genetically modified to express BDNF promote axonal regeneration from supraspinal neurons following chronic spinal cord injury publication-title: Exp Neurol – volume: 148 start-page: 444 issue: 2 year: 1997b end-page: 452 article-title: Robust growth of chronically injured spinal cord axons induced by grafts of genetically modified NGF‐secreting cells publication-title: Exp Neurol – year: 2009 article-title: Fibrin‐based tissue engineering scaffolds enhance neural fiber sprouting and delay the accumulation of reactive astrocytes at the lesion in a subacute model of spinal cord injury publication-title: J Biomed Mater Res A – volume: 27 start-page: 3560 issue: 19 year: 2006 end-page: 3569 article-title: The promotion of oriented axonal regrowth in the injured spinal cord by alginate‐based anisotropic capillary hydrogels publication-title: Biomaterials – volume: 23 start-page: 264 issue: 3–4 year: 2006 end-page: 280 article-title: Degenerative and spontaneous regenerative processes after spinal cord injury publication-title: J Neurotrauma – volume: 116 start-page: 204 issue: 2 year: 2006 end-page: 210 article-title: Effect of controlled delivery of neurotrophin‐3 from fibrin on spinal cord injury in a long term model publication-title: J Control Release – volume: 359 start-page: 417 issue: 9304 year: 2002 end-page: 425 article-title: Spinal‐cord injury publication-title: Lancet – volume: 25 start-page: 1569 issue: 9 year: 2004 end-page: 1582 article-title: Freeze‐dried poly(D,L‐lactic acid) macroporous guidance scaffolds impregnated with brain‐derived neurotrophic factor in the transected adult rat thoracic spinal cord publication-title: Biomaterials – volume: 201 start-page: 359 issue: 2 year: 2006 end-page: 367 article-title: An injectable, biodegradable hydrogel for trophic factor delivery enhances axonal rewiring and improves performance after spinal cord injury publication-title: Exp Neurol – volume: 17 start-page: 5560 issue: 14 year: 1997a end-page: 5572 article-title: Cellular delivery of neurotrophin‐3 promotes corticospinal axonal growth and partial functional recovery after spinal cord injury publication-title: J Neurosci – volume: 540 start-page: 183 issue: 1–2 year: 1991 end-page: 194 article-title: Axonal growth and glial migration from co‐cultured hippocampal and septal slices into fibrin‐fibronectin‐containing matrix of peripheral regeneration chambers: A light and electron microscope study publication-title: Brain Res – volume: 23 start-page: 617 issue: 5 year: 2006 end-page: 634 article-title: Delayed intervention with transplants and neurotrophic factors supports recovery of forelimb function after cervical spinal cord injury in adult rats publication-title: J Neurotrauma – volume: 452 start-page: 255 issue: 3 year: 2002 end-page: 263 article-title: Differential effects of neurotrophins on neuronal survival and axonal regeneration after spinal cord injury in adult rats publication-title: J Comp Neurol – volume: 98 start-page: 281 issue: 2 year: 2004 end-page: 294 article-title: Controlled release of neurotrophin‐3 from fibrin gels for spinal cord injury publication-title: J Control Release – ident: e_1_2_1_2_1 doi: 10.1006/exnr.2000.7413 – ident: e_1_2_1_17_1 doi: 10.1016/S0142-9612(03)00503-9 – ident: e_1_2_1_11_1 doi: 10.1002/jbm.a32343 – ident: e_1_2_1_18_1 doi: 10.1016/j.expneurol.2006.04.020 – ident: e_1_2_1_24_1 doi: 10.1016/j.jconrel.2004.05.003 – ident: e_1_2_1_12_1 doi: 10.1016/0006-8993(91)90506-Q – ident: e_1_2_1_8_1 doi: 10.1002/jnr.20662 – ident: e_1_2_1_23_1 doi: 10.1016/j.jconrel.2006.07.005 – ident: e_1_2_1_28_1 doi: 10.1016/S0306-4522(02)00384-6 – ident: e_1_2_1_14_1 doi: 10.1089/neu.2006.23.617 – ident: e_1_2_1_22_1 doi: 10.1016/S0014-4886(03)00398-4 – volume: 27 start-page: 3560 issue: 19 year: 2006 ident: e_1_2_1_19_1 article-title: The promotion of oriented axonal regrowth in the injured spinal cord by alginate‐based anisotropic capillary hydrogels publication-title: Biomaterials contributor: fullname: Prang P – ident: e_1_2_1_21_1 doi: 10.1038/367170a0 – ident: e_1_2_1_13_1 doi: 10.1016/j.expneurol.2006.07.030 – ident: e_1_2_1_4_1 doi: 10.1016/S0361-9230(99)00072-6 – ident: e_1_2_1_5_1 doi: 10.1523/JNEUROSCI.17-14-05560.1997 – ident: e_1_2_1_7_1 doi: 10.1089/neu.2006.23.263 – ident: e_1_2_1_6_1 doi: 10.1006/exnr.1997.6704 – ident: e_1_2_1_16_1 doi: 10.1002/cne.10381 – ident: e_1_2_1_27_1 doi: 10.1006/exnr.1998.6912 – ident: e_1_2_1_10_1 doi: 10.1006/exnr.2002.7980 – ident: e_1_2_1_26_1 doi: 10.1016/S0014-4886(03)00394-7 – ident: e_1_2_1_9_1 doi: 10.1016/j.biomaterials.2005.07.008 – ident: e_1_2_1_25_1 doi: 10.1016/j.jconrel.2006.05.005 – ident: e_1_2_1_15_1 doi: 10.1016/S0140-6736(02)07603-1 – ident: e_1_2_1_3_1 doi: 10.1523/JNEUROSCI.21-23-09334.2001 – ident: e_1_2_1_20_1 doi: 10.1016/S0168-3659(99)00221-7 |
| SSID | ssj0007866 |
| Score | 2.3610864 |
| Snippet | This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin-3 (NT-3) from fibrin scaffolds can stimulate... This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin‐3 (NT‐3) from fibrin scaffolds can stimulate... Abstract This study investigated whether delayed treatment of spinal cord injury with controlled release of neurotrophin‐3 (NT‐3) from fibrin scaffolds can... |
| SourceID | pubmedcentral proquest crossref pubmed pascalfrancis wiley istex fao |
| SourceType | Open Access Repository Aggregation Database Index Database Publisher |
| StartPage | 1207 |
| SubjectTerms | Animals Biological and medical sciences biomaterial Biotechnology delivery system Female Fibrin Fundamental and applied biological sciences. Psychology growth factors Medical treatment Nerve Fibers - drug effects Nerve Regeneration Neurons Neurotrophin 3 - pharmacokinetics Rats Rats, Long-Evans Rodents Spinal Cord - pathology Spinal cord injuries Spinal Cord Injuries - therapy Studies Tissue Engineering - methods Tissue Scaffolds - chemistry |
| Title | Controlled release of neurotrophin-3 from fibrin-based tissue engineering scaffolds enhances neural fiber sprouting following subacute spinal cord injury |
| URI | https://api.istex.fr/ark:/67375/WNG-L34MC1HT-2/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1002%2Fbit.22476 https://www.ncbi.nlm.nih.gov/pubmed/19603426 https://www.proquest.com/docview/213760621 https://search.proquest.com/docview/743658319 https://search.proquest.com/docview/746080130 https://pubmed.ncbi.nlm.nih.gov/PMC2780336 |
| Volume | 104 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV3dbtMwFLbGJARc8NMBC4PJQmjiJl1sN04irrbCKIjtAlqxCyTLTmxaVtJpacXPFY_AC_ByPAnnOE278ifEXRLbUex88flOfPwdQh5E0iWxljwscqvDjjY8zOI4DaXTeZ7GzvAUNzgfHsneoPP8OD5eI4-avTC1PsTihxt-GX6-xg9cm2p3KRpqRtM22J8E5baZSDCc6_HLpXRUktbrlOgxizjjjapQxHcXLVds0QWnJ8BQcXA_YoSkrmCQXJ3d4nf089coyvPs1pung2vkTdOxOirlpD2bmnb--SfNx__s-XVydU5b6V6NsxtkzZYtsrFXgsv-_hPdoT6Q1P-hb5GL-83RpW6TTq5FrpxTPtwg37p1iPzYFhTTtoAtpRNHvbomXD8djsrvX74KirtfqMNtCXiOJregU48Vapf3ozCIzk3GRQVXh4jjyt8KnthhRAytYFxmGOBNodZ48sG3mRmdz6YWCjEzGEU3nI7KdwCym2Rw8KTf7YXzTBFhDoxJhnlmbBy5OGdCamZkVLC0AHDmHaYlMLBCisQZLSRKDbnUGcatBJOTCZ1mBRfiFlkvJ6XdJJTZPEsjZ3LLHHiDJmMmKYoCsytFJk9tQO43mFGntSCIqqWfuYIXo_yLCcgmoEnptzBRq8ErjsvDKIQIvndAdjzEFo312QkG1yWxen30VL0QncMu6_UVD8j2CgYXDTh6kUBcA7LVgFLNJ55KcYZRTpKzgNBFKbxpXAbSpZ3MKgWcEWgnTL1_qyLBk4CHDsjtGuTLvoLLK4DWBSRZgf-iAuqVr5aUo6HXLedJGgkBLR96dP95-NT-s74_uPPvVbfIZT5P7cHiu2R9ejaz94AvTs22nxh-APGEbAk |
| link.rule.ids | 230,315,783,787,888,1378,27937,27938,46307,46731 |
| linkProvider | Wiley-Blackwell |
| linkToHtml | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Lb9QwEB61Rahw4FEeDYViIVRxSZvYGyeRuLQLZQttD7AVvSDLTmy6tGSr7q54nPgJ_AH-HL-EGWez2-UlxC2J7ciPz54Ze_wNwMNIujTRkodlYXXY0oaHeZJkoXS6KLLEGZ7RBee9fdk5aD0_TA7n4HFzF6bmh5hsuNHM8Os1TXDakN6Ysoaa3nAdBVAq5-ECTndB0_LJyyl5VJrVJ5VkM4sk5w2vUMQ3JkVnpNG8033UUal7P5KPpB5gN7k6vsXvFNBf_SjP67deQG1fhTdN02q_lOP10dCsF59_Yn3837ZfgytjzZVt1lC7DnO2WoIbmxVa7e8_sTXmfUn9Jv0SXNxqnhbbTUS5Jbh8jvzwBnxr117yJ7ZkFLkFxSnrO-YJNvH76VGv-v7lq2B0AYY5uplA7yR1Szb0cGF2-j-Gvehc_6Qc4NcjgvLA_wpr7Mgphg2wY0bk480w10n_gy8zMroYDS0mUnAwRpY461XvEGc34WD7abfdCcfBIsIClSYZFrmxSeSSIhZSx0ZGZZyViM-iFWuJSlgpReqMFpLYhlzmTMytRKmTC53lJRfiFixU_couA4ttkWeRM4WNHRqEJo9NWpYlBViKTJHZAB40oFGnNSeIqtmfucKBUX5gAlhGOCn9FtdqdfCK0wkxcSGi-R3AmsfYpLA-Oyb_ujRRr_efqV3R2mvHna7iAazOgHBSgJMhibprACsNKtV47RkoHpOjk-RxAGySiiNNJ0G6sv3RQKHaiJonrr5_yyLRmMBKB3C7Rvm0rWj1CtTsAkhn8D_JQJTlsylV78hTl_M0i4TAko88vP_cfWprp-sf7vx71vuw2Onu7ardnf0XK3CJjyN9xMldWBiejew9VB-HZtWvEj8AVTdwIg |
| linkToPdf | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1bb9MwFD7ahrg9cNmAhcGwEJp4yZY4jZOIp61QOtgqBJ3YA5JlJzYrG2m1tuLyxE_gD_Dn-CWc4zTtyk2ItyS2o9j54vOd-Pg7AA8CYZNYCe4XuVF-Q2nuZ3Gc-sKqPE9jq3lKG5z3O6J90Hh2GB8uwKN6L0ylDzH94UZfhpuv6QMfFHZrJhqqe6NNtD-JWIRzDYFQJUb0cqYdlaTVQiW5zFGc8VpWKOBb06ZzxmjRqj5SVBrdjxQiqYY4SrZKb_E7_vlrGOVZeuvsU-sqvKl7VoWlHG-OR3oz__yT6ON_dv0aXJnwVrZdAe06LJhyGVa2S_TZ339iG8xFkrpf9Mtwfqc-utis88ktw-Uz0ocr8K1ZxcifmIJR3hY0pqxvmZPXxOuDo175_cvXiNH2F2ZpXwKdk80t2MiBhZnZ_RgOorX9k2KIV48IyEN3K3xiSyExbIjjMqYIb4a1TvofXJuxVvl4ZLCQUoMx8sNZr3yHKLsBB60n3Wbbn6SK8HOkTMLPM23iwMZ5GAkVahEUYVogOvNGqARSsEJEidUqEqQ1ZFOrQ24E2pwsUmlW8Ci6CUtlvzSrwEKTZ2lgdW5Ci-6gzkKdFEVB6ZUCnafGg_s1ZuSgUgSRlfYzl_hipHsxHqwimqR6izO1PHjFaX2YlBDR-fZgw0Fs2lidHlN0XRLL152nci9q7DfDdldyD9bnMDhtwMmNRObqwVoNSjmZeYaShxTmJHjoAZuW4pumdSBVmv54KJE0Iu_EufdvVQS6EvjQHtyqQD7rK_q8EfI6D5I5-E8rkGD5fEnZO3LC5TxJgyjClg8duv88fHJnt-sObv971Xtw4cXjltzb7Txfg0t8kuYjjO_A0uh0bO4idxzpdTdH_AC_6m7R |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Controlled+release+of+neurotrophin%E2%80%903+from+fibrin%E2%80%90based+tissue+engineering+scaffolds+enhances+neural+fiber+sprouting+following+subacute+spinal+cord+injury&rft.jtitle=Biotechnology+and+bioengineering&rft.au=Johnson%2C+Philip+J.&rft.au=Parker%2C+Stanley+R.&rft.au=Sakiyama%E2%80%90Elbert%2C+Shelly+E.&rft.date=2009-12-15&rft.issn=0006-3592&rft.eissn=1097-0290&rft.volume=104&rft.issue=6&rft.spage=1207&rft.epage=1214&rft_id=info:doi/10.1002%2Fbit.22476&rft.externalDBID=n%2Fa&rft.externalDocID=10_1002_bit_22476 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3592&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3592&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3592&client=summon |