Injectable alginate-microencapsulated canine adipose tissue-derived mesenchymal stem cells for enhanced viable cell retention
The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were...
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Published in | Journal of Veterinary Medical Science Vol. 79; no. 3; pp. 492 - 501 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
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Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
2017
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
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Abstract | The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro. HEK-293 cells, with or without microencapsulation, were injected into the subcutaneous tissue of mice and were tracked using in vivo bioluminescent imaging to evaluate the retention of transplanted cells. The optimized injectable microbeads were of uniform size and approximately 250 µm in diameter. There was no strong evidence of in vivo toxicity for the alginate beads. The cells remained viable after encapsulation, and there was evidence of in vitro proliferation within the microcapsules. In vivo bioluminescent imaging showed that alginate encapsulation improved the retention of transplanted cells and the encapsulated cells remained viable in vivo for 7 days. Encapsulation enhances the retention of viable cells in vivo and might represent a potential strategy to increase the therapeutic potency and efficacy of stem cells. |
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AbstractList | The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro. HEK-293 cells, with or without microencapsulation, were injected into the subcutaneous tissue of mice and were tracked using in vivo bioluminescent imaging to evaluate the retention of transplanted cells. The optimized injectable microbeads were of uniform size and approximately 250 µm in diameter. There was no strong evidence of in vivo toxicity for the alginate beads. The cells remained viable after encapsulation, and there was evidence of in vitro proliferation within the microcapsules. In vivo bioluminescent imaging showed that alginate encapsulation improved the retention of transplanted cells and the encapsulated cells remained viable in vivo for 7 days. Encapsulation enhances the retention of viable cells in vivo and might represent a potential strategy to increase the therapeutic potency and efficacy of stem cells. The purpose of this study was to establish an optimized protocol for the production of alginate-encapsulated canine adipose-derived mesenchymal stem cells (cASCs) and evaluate their suitability for clinical use, including viability, proliferation and in vivo cell retention. Alginate microbeads were formed by vibrational technology and the production of injectable microbeads was performed using various parameters with standard methodology. Microbead toxicity was tested in an animal model. Encapsulated cASCs were evaluated for viability and proliferation in vitro . HEK-293 cells, with or without microencapsulation, were injected into the subcutaneous tissue of mice and were tracked using in vivo bioluminescent imaging to evaluate the retention of transplanted cells. The optimized injectable microbeads were of uniform size and approximately 250 µ m in diameter. There was no strong evidence of in vivo toxicity for the alginate beads. The cells remained viable after encapsulation, and there was evidence of in vitro proliferation within the microcapsules. In vivo bioluminescent imaging showed that alginate encapsulation improved the retention of transplanted cells and the encapsulated cells remained viable in vivo for 7 days. Encapsulation enhances the retention of viable cells in vivo and might represent a potential strategy to increase the therapeutic potency and efficacy of stem cells. |
Author | KOH, Eunji JUNG, Yun Chan WOO, Heung-Myong KANG, Byung-Jae |
Author_xml | – sequence: 1 fullname: KOH, Eunji organization: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea – sequence: 2 fullname: JUNG, Yun Chan organization: KPC, Gwangju 12773, Republic of Korea – sequence: 3 fullname: WOO, Heung-Myong organization: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea – sequence: 4 fullname: KANG, Byung-Jae organization: College of Veterinary Medicine and Institute of Veterinary Science, Kangwon National University, Chuncheon 24341, Republic of Korea |
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Cites_doi | 10.1016/j.jconrel.2014.05.010 10.46582/jsrm.1002007 10.1016/j.tvjl.2014.09.024 10.1098/rsif.2014.0817 10.1016/j.biomaterials.2010.05.078 10.1016/j.jconrel.2008.08.010 10.1016/j.bbrc.2006.06.072 10.1089/ten.tec.2008.0582 10.3390/ma6041285 10.1371/journal.pone.0023212 10.1002/term.1680 10.4142/jvs.2012.13.3.299 10.1007/s11095-009-9884-4 10.1371/journal.pone.0107001 10.1096/fj.12-213611 10.1371/journal.pone.0113609 10.1161/JAHA.113.000367 10.1371/journal.pone.0062032 10.3727/096368912X637497 10.1111/wrr.12044 10.1016/j.msec.2013.11.048 10.1016/j.tiv.2014.09.014 10.1292/jvms.12-0065 |
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SubjectTerms | Adipose tissue Adipose Tissue - cytology alginate Alginates Alginates - toxicity Alginic acid Animal models Animal tissues Animals Biocompatible Materials canine Capsules - administration & dosage Capsules - chemistry Capsules - toxicity Cell Proliferation Cell Separation - veterinary Cell Survival Dogs - anatomy & histology Encapsulation Glucuronic Acid - toxicity HEK293 Cells Hexuronic Acids - toxicity Humans injectable Male mesenchymal stem cell Mesenchymal Stem Cell Transplantation - veterinary Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchyme Mice Mice, Inbred ICR Microcapsules Microencapsulation Microspheres Rats Rats, Sprague-Dawley Retention Stem cell transplantation Stem cells Surgery Toxicity Toxicity testing Viability |
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Title | Injectable alginate-microencapsulated canine adipose tissue-derived mesenchymal stem cells for enhanced viable cell retention |
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