Formalin Inactivation of Japanese Encephalitis Virus Vaccine Alters the Antigenicity and Immunogenicity of a Neutralization Epitope in Envelope Protein Domain III

• Published in: PLoS neglected tropical diseases Vol. 9; no. 10; p. e0004167
• Main Authors: Fan, Yi-Chin, Chiu, Hsien-Chung, Chen, Li-Kuang, Chang, Gwong-Jen J., Chiou, Shyan-Song
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.10.2015; Public Library of Science (PLoS)
• Subjects: Animals; Antibodies, Neutralizing - blood; Antibodies, Viral - blood; Antigenic determinants; Antigens, Viral - immunology; Care and treatment; Complications and side effects; Development and progression; Disinfectants - pharmacology; Encephalitis; Encephalitis Virus, Japanese - drug effects; Epitopes - immunology; Formaldehyde; Formaldehyde - pharmacology; Funding; Genotype & phenotype; Immunoglobulins; Infections; Japanese encephalitis; Japanese Encephalitis Vaccines - immunology; Membrane Glycoproteins - immunology; Mice, Inbred BALB C; Monoclonal antibodies; Patient outcomes; Plasmids; Prevention; Proteins; Vaccines; Vaccines, Inactivated - immunology; Viral Envelope Proteins - immunology; Viral infections; Virus Inactivation; Viruses; Taiwan

Formalin-inactivated Japanese encephalitis virus (JEV) vaccines are widely available, but the effects of formalin inactivation on the antigenic structure of JEV and the profile of antibodies elicited after vaccination are not well understood. We used a panel of monoclonal antibodies (MAbs) to map th...