Integrating vectors for genetic studies in the rare Actinomycete Amycolatopsis marina

• Published in: BMC biotechnology Vol. 19; no. 1; p. 32
• Main Authors: Gao, Hong, Murugesan, Buvani, Hoßbach, Janina, Evans, Stephanie K, Stark, W Marshall, Smith, Margaret C M
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 04.06.2019; BioMed Central; BMC
• Subjects: Actinobacteria - genetics; Actinobacteria - virology; Actinomycetes; Amycolatopsis; Antibiotics; Attachment Sites, Microbiological - genetics; Base Sequence; Cloning; Conjugation; Deoxyribonucleic acid; DNA; DNA sequencing; E coli; Escherichia coli; Gene sequencing; Genetic aspects; Genetic engineering; Genetic vectors; Genetic Vectors - genetics; Genome, Bacterial - genetics; Genomes; Genomics; Homology; Integrases; Integrases - genetics; Integrases - metabolism; Integrating vectors; Laboratories; Loci; Metabolism; Natural products; Phages; Plasmids; R4 integrase; Rare Actinomycetes; Recombination; Recombination, Genetic; Sequence Homology, Nucleic Acid; Streptomyces; TG1 integrase; Vectors; Viral Proteins - genetics; Viral Proteins - metabolism; Integrating vectors; Rare Actinomycetes; R4 integrase; Amycolatopsis; TG1 integrase

Few natural product pathways from rare Actinomycetes have been studied due to the difficulty in applying molecular approaches in these genetically intractable organisms. In this study, we sought to identify more integrating vectors, using phage int/attP loci, that would efficiently integrate site-sp...