Identification of Binding Proteins of Fusarielin A as Actin and Tubulin

• Published in: Chemical & Pharmaceutical Bulletin Vol. 58; no. 1; pp. 129 - 134
• Main Authors: Noguchi-Yachide, Tomomi, Dodo, Kosuke, Aoyama, Hiroshi, Fujimoto, Haruka, Hori, Masatoshi, Hashimoto, Yuichi, Kobayashi, Hisayoshi
• Format: Journal Article
• Language: English; Japanese
• Published: TOKYO The Pharmaceutical Society of Japan 01.01.2010; Pharmaceutical Society of Japan; Pharmaceutical Soc Japan; Japan Science and Technology Agency
• Subjects: actin; Actins - analysis; Actins - metabolism; affinity gel; Amino Acid Sequence; Angiogenesis Inhibitors - isolation & purification; Angiogenesis Inhibitors - pharmacology; Antifungal Agents - isolation & purification; Antifungal Agents - pharmacology; binding protein; Cell Line; Chemistry; Chemistry, Medicinal; Chemistry, Multidisciplinary; Epoxy Compounds - isolation & purification; Epoxy Compounds - pharmacology; fusarielin A; Fusarium - chemistry; HL-60; Humans; Life Sciences & Biomedicine; Molecular Sequence Data; Naphthalenes - isolation & purification; Naphthalenes - pharmacology; Pharmacology & Pharmacy; Physical Sciences; Protein Binding; Science & Technology; tubulin; Tubulin - analysis; Tubulin - metabolism; CYTOSKELETAL; actin; PURIFICATION; affinity gel; tubulin; MACROCYCLIC LACTONE ANTIBIOTICS; RHIZOXIN; HL-60; fusarielin A; INHIBITOR; binding protein; BRAIN
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.58.129

Fusarielin A (FSA), isolated from culture of Fusarium sp. K432, has anti-fungal/anti-angiogenic activity. Based on our previous structure–activity relationship studies, we prepared FSA-immobilized affinity gel. The results of affinity chromatography with this gel indicated that tubulin and actin are FSA-binding proteins. The binding affinities of tubulin and actin for FSA were estimated by the use of surface plasmon resonance spectroscopy.