Immunohistochemical analysis for acetylcholinesterase and choline acetyltransferase in mouse cerebral cortex after traumatic brain injury

• Published in: Journal of Veterinary Medical Science Vol. 82; no. 6; pp. 827 - 835
• Main Authors: HORIO, Tomoyo, OZAWA, Aisa, KAMIIE, Junichi, SAKAUE, Motoharu
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2020; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: acetylcholine; Acetylcholinesterase; Acetylcholinesterase - metabolism; Acetyltransferase; Anatomy; Animals; Antigens, CD - metabolism; Antigens, Differentiation, Myelomonocytic - metabolism; Astrocytes; Brain - enzymology; Brain - metabolism; Brain Injuries, Traumatic - physiopathology; Cell death; Central nervous system; Cerebral cortex; Cerebral Cortex - enzymology; Cerebral Cortex - metabolism; Choline; choline acetyltransferase; Choline O-acetyltransferase; Choline O-Acetyltransferase - metabolism; Enzymes; Glial cells; Glial Fibrillary Acidic Protein; Immunohistochemistry; Male; Mice, Inbred C57BL; Microglia; Neuroglia - metabolism; Neuroglia - pathology; Neuronal-glial interactions; Traumatic brain injury; traumatic brain injury; acetylcholinesterase; choline acetyltransferase; acetylcholine; immunohistochemistry

The regulation of glial cells, especially astrocytes and microglia, is important to prevent the exacerbation of a brain injury because over-reactive glial cells promote neuronal death. Acetylcholine (ACh), a neurotransmitter synthesized and hydrolyzed by choline acetyltransferase (ChAT) and acetylch...