The crystal structure of bromide-bound GtACR1 reveals a pre-activated state in the transmembrane anion tunnel

• Published in: eLife Vol. 10
• Main Authors: Li, Hai, Huang, Chia-Ying, Govorunova, Elena G, Sineshchekov, Oleg A, Yi, Adrian, Rothschild, Kenneth J, Wang, Meitian, Zheng, Lei, Spudich, John L
• Format: Journal Article
• Language: English
• Published: Cambridge eLife Science Publications, Ltd 17.05.2021; eLife Sciences Publications Ltd; eLife Sciences Publications, Ltd
• Subjects: ACR; Binding sites; bromide binding; Channel opening; Channel pores; channelrhodopsin; Chloride; Crystal structure; Crystals; Data collection; Free energy; GtACR1; Guillardia theta; optogenetics; Proteins; Research Advance; Structural Biology and Molecular Biophysics; Structure; Canada

The crystal structure of the light-gated anion channel GtACR1 reported in our previous Research Article (Li et al., 2019) revealed a continuous tunnel traversing the protein from extracellular to intracellular pores. We proposed the tunnel as the conductance channel closed by three constrictions: C1...