Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses

• Published in: Journal of Veterinary Medical Science Vol. 86; no. 4; pp. 389 - 395
• Main Authors: MIMURA, Yume, HIONO, Takahiro, HUYNH, Loc Tan, OGINO, Saho, KOBAYASHI, Maya, ISODA, Norikazu, SAKODA, Yoshihiro
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 2024; Japan Science and Technology Agency; The Japanese Society of Veterinary Science
• Subjects: Animals; Biotypes; Border disease; Cell culture; classical swine fever virus; Classical Swine Fever Virus - genetics; Hog cholera; infectivity titer; luciferase activity; Luciferases - genetics; pestivirus; Pestivirus - genetics; Superinfection; Superinfection - veterinary; superinfection exclusion; Swine; Swine Diseases; Titration; Virology; Viruses; superinfection exclusion; classical swine fever virus; pestivirus; infectivity titer; luciferase activity

Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies...