Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses
Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies...
Saved in:
| Published in | Journal of Veterinary Medical Science Vol. 86; no. 4; pp. 389 - 395 |
|---|---|
| Main Authors | , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Japan
JAPANESE SOCIETY OF VETERINARY SCIENCE
2024
Japan Science and Technology Agency The Japanese Society of Veterinary Science |
| Subjects | |
| Online Access | Get full text |
Cover
Loading…
| Abstract | Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE−/HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. |
|---|---|
| AbstractList | Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE−/HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE-/HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system.Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE-/HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE /HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their titration requires alternative methods rather than direct observation of cytopathogenic effects, such as immunostaining using specific antibodies or interference with cytopathogenic strains. However, these methods require microscopic observation to assess virus growth, which is time- and labor-intensive, especially when handling several samples. In this study, we developed a novel luciferase-based pestivirus titration method using the superinfection exclusion phenomenon with recombinant reporter pestiviruses that possessed an 11-amino-acid subunit derived from NanoLuc luciferase (HiBiT). In this method, swine kidney cells were inoculated with classical swine fever virus (CSFV) and superinfected with the reporter CSFV vGPE − /HiBiT 5 days postinoculation. Virus titer was determined based on virus growth measured in luminescence using the culture fluid 3 days after superinfection; the resultant virus titer was comparable to that obtained by immunoperoxidase staining. Furthermore, this method has proven to be applicable for the titration of border disease virus (BDV) by superinfection with both the homologous reporter BDV and heterologous reporter CSFV, suggesting that this novel virus titration method is a simple technique for automated virus detection based on the luciferase system. |
| ArticleNumber | 24-0005 |
| Author | HIONO, Takahiro KOBAYASHI, Maya MIMURA, Yume OGINO, Saho ISODA, Norikazu SAKODA, Yoshihiro HUYNH, Loc Tan |
| Author_xml | – sequence: 1 fullname: MIMURA, Yume organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 2 fullname: HIONO, Takahiro organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 3 fullname: HUYNH, Loc Tan organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 4 fullname: OGINO, Saho organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 5 fullname: KOBAYASHI, Maya organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 6 fullname: ISODA, Norikazu organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan – sequence: 7 fullname: SAKODA, Yoshihiro organization: Laboratory of Microbiology, Department of Disease Control, Faculty of Veterinary Medicine, Hokkaido University, Hokkaido, Japan |
| BackLink | https://www.ncbi.nlm.nih.gov/pubmed/38355118$$D View this record in MEDLINE/PubMed |
| BookMark | eNp1kc1v1DAUxC1URLeFG2cUiUsPpPVXnPiEoCoUqVIvcLYc52XXq8QOtrOC_75Od1mVSr3Ylt7vjWc0Z-jEeQcIvSf4klBJr7a7MV5SXmKMq1doRRivy5ozeYJWWBJR1rTCp-gsxi3GlHAh36BT1rCqIqRZofkmJt0ONm5GcKnwfaGLOE8QrOvBJOtdAX_MMMflNULa-K7ofSgmiMnubJhjkWwK-pHMlFtngQDGj611OisGmHxI8HQD4lv0utdDhHeH-xz9-nbz8_q2vLv__uP6y11phCCplJpno4Iy2hoOumFQ9aB7KbEwgtQ15m3bamZwx7qmaY3RLc2pur7THChu2Dn6vNed5naEzuSIQQ9qCnbU4a_y2qr_J85u1NrvFCFYkKoWWeHioBD87zlHUKONBoZBO_BzVLmBmhImsczox2fo1s_B5XyK4YrnHiRfLH14auno5V8lGfi0B0zwMQbojwjBamlcLY0rytXSeMbpM9zY9FhHDmSHl5a-7pe2uf01HH_QIVkzwB5uhOLLcVg6Ds1GBwWOPQDTUswL |
| CitedBy_id | crossref_primary_10_3390_v16121834 |
| Cites_doi | 10.1128/JVI.01191-19 10.1016/0042-6822(92)90199-Y 10.1128/jvi.69.6.3683-3689.1995 10.1128/JVI.01748-06 10.1128/JVI.77.7.4283-4290.2003 10.1128/jvi.68.5.3289-3297.1994 10.1128/JVI.01205-09 10.1038/341491a0 10.1128/JVI.00089-16 10.1128/jvi.54.2.351-357.1985 10.1016/j.jviromet.2006.08.005 10.1099/0022-1317-79-12-2981 10.1128/JVI.72.6.5318-5322.1998 10.1016/0378-1135(92)90051-T 10.1128/JCM.00859-13 10.3390/pathogens9030188 10.1016/j.virusres.2019.197809 10.1128/JVI.74.24.11792-11799.2000 10.1128/JVI.01582-17 10.1016/j.antiviral.2014.06.006 10.1021/acschembio.5b00753 10.1016/S0166-0934(98)00098-6 10.1021/acschembio.7b00549 10.1371/journal.pone.0149469 10.1007/s11626-998-0053-6 10.1093/oxfordjournals.aje.a118408 10.1099/vir.0.83041-0 10.3181/00379727-110-27481 10.1099/vir.0.18773-0 10.1016/j.cub.2005.02.058 10.1128/JVI.79.6.3231-3242.2005 10.1292/jvms.63.751 10.1128/JVI.00079-08 |
| ContentType | Journal Article |
| Copyright | 2024 by the Japanese Society of Veterinary Science 2024. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. 2024 The Japanese Society of Veterinary Science 2024 |
| Copyright_xml | – notice: 2024 by the Japanese Society of Veterinary Science – notice: 2024. This work is published under https://creativecommons.org/licenses/by-nc-nd/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. – notice: 2024 The Japanese Society of Veterinary Science 2024 |
| DBID | AAYXX CITATION CGR CUY CVF ECM EIF NPM 7QR 7U9 8FD FR3 H94 M7N P64 7X8 5PM |
| DOI | 10.1292/jvms.24-0005 |
| DatabaseName | CrossRef Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed Chemoreception Abstracts Virology and AIDS Abstracts Technology Research Database Engineering Research Database AIDS and Cancer Research Abstracts Algology Mycology and Protozoology Abstracts (Microbiology C) Biotechnology and BioEngineering Abstracts MEDLINE - Academic PubMed Central (Full Participant titles) |
| DatabaseTitle | CrossRef MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) Virology and AIDS Abstracts Technology Research Database Algology Mycology and Protozoology Abstracts (Microbiology C) AIDS and Cancer Research Abstracts Chemoreception Abstracts Engineering Research Database Biotechnology and BioEngineering Abstracts MEDLINE - Academic |
| DatabaseTitleList | Virology and AIDS Abstracts MEDLINE - Academic MEDLINE |
| Database_xml | – sequence: 1 dbid: NPM name: PubMed url: https://proxy.k.utb.cz/login?url=http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=PubMed sourceTypes: Index Database – sequence: 2 dbid: EIF name: MEDLINE url: https://proxy.k.utb.cz/login?url=https://www.webofscience.com/wos/medline/basic-search sourceTypes: Index Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Veterinary Medicine |
| EISSN | 1347-7439 |
| EndPage | 395 |
| ExternalDocumentID | PMC11061576 38355118 10_1292_jvms_24_0005 article_jvms_86_4_86_24_0005_article_char_en |
| Genre | Journal Article |
| GroupedDBID | 29L 2WC 53G 5GY ACGFO ACIWK ACPRK ADBBV ADRAZ AENEX AFRAH AI. ALMA_UNASSIGNED_HOLDINGS AOIJS B.T BAWUL CS3 DIK DU5 E3Z EBS EJD HYE JSF JSH KQ8 M48 M~E N5S OK1 P2P RJT RNS RPM RYR RZJ TKC TR2 VH1 XSB AAYXX CITATION OVT PGMZT CGR CUY CVF ECM EIF NPM 7QR 7U9 8FD FR3 H94 M7N P64 7X8 5PM |
| ID | FETCH-LOGICAL-c661t-9a48356232bc4ea83e5feaf9906c617704bbba3c0d3d88bccab2118dfda4e2083 |
| IEDL.DBID | M48 |
| ISSN | 0916-7250 1347-7439 |
| IngestDate | Thu Aug 21 18:34:29 EDT 2025 Fri Jul 11 12:24:18 EDT 2025 Sun Jun 29 13:37:42 EDT 2025 Mon Jul 21 06:02:54 EDT 2025 Tue Jul 01 00:31:12 EDT 2025 Thu Apr 24 23:01:02 EDT 2025 Fri May 10 01:25:10 EDT 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 4 |
| Keywords | superinfection exclusion classical swine fever virus pestivirus infectivity titer luciferase activity |
| Language | English |
| License | This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
| LinkModel | DirectLink |
| MergedId | FETCHMERGED-LOGICAL-c661t-9a48356232bc4ea83e5feaf9906c617704bbba3c0d3d88bccab2118dfda4e2083 |
| Notes | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 14 content type line 23 |
| OpenAccessLink | http://journals.scholarsportal.info/openUrl.xqy?doi=10.1292/jvms.24-0005 |
| PMID | 38355118 |
| PQID | 3054725948 |
| PQPubID | 2028964 |
| PageCount | 7 |
| ParticipantIDs | pubmedcentral_primary_oai_pubmedcentral_nih_gov_11061576 proquest_miscellaneous_2927213909 proquest_journals_3054725948 pubmed_primary_38355118 crossref_primary_10_1292_jvms_24_0005 crossref_citationtrail_10_1292_jvms_24_0005 jstage_primary_article_jvms_86_4_86_24_0005_article_char_en |
| ProviderPackageCode | CITATION AAYXX |
| PublicationCentury | 2000 |
| PublicationDate | 2024-00-00 |
| PublicationDateYYYYMMDD | 2024-01-01 |
| PublicationDate_xml | – year: 2024 text: 2024-00-00 |
| PublicationDecade | 2020 |
| PublicationPlace | Japan |
| PublicationPlace_xml | – name: Japan – name: Tokyo |
| PublicationTitle | Journal of Veterinary Medical Science |
| PublicationTitleAlternate | J. Vet. Med. Sci. |
| PublicationYear | 2024 |
| Publisher | JAPANESE SOCIETY OF VETERINARY SCIENCE Japan Science and Technology Agency The Japanese Society of Veterinary Science |
| Publisher_xml | – name: JAPANESE SOCIETY OF VETERINARY SCIENCE – name: Japan Science and Technology Agency – name: The Japanese Society of Veterinary Science |
| References | 36. Zou G, Zhang B, Lim PY, Yuan Z, Bernard KA, Shi PY. 2009. Exclusion of West Nile virus superinfection through RNA replication. J Virol 83: 11765–11776. 3. Criddle A, Thornburg T, Kochetkova I, DePartee M, Taylor MP. 2016. gD-independent superinfection exclusion of alphaherpesviruses. J Virol 90: 4049–4058. 28. Sakoda Y, Hikawa M, Tamura T, Fukusho A. 1998. Establishment of a serum-free culture cell line, CPK-NS, which is useful for assays of classical swine fever virus. J Virol Methods 75: 59–68. 17. Meyers G, Rümenapf T, Thiel HJ. 1989. Ubiquitin in a togavirus. Nature 341: 491. 15. Li Y, Shen L, Sun Y, Yuan J, Huang J, Li C, Li S, Luo Y, Qiu HJ. 2013. Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus. J Clin Microbiol 51: 2710–2712. 6. Geib T, Sauder C, Venturelli S, Hässler C, Staeheli P, Schwemmle M. 2003. Selective virus resistance conferred by expression of Borna disease virus nucleocapsid components. J Virol 77: 4283–4290. 27. Sakoda Y, Fukusho A. 1998. Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture. In Vitro Cell Dev Biol Anim 34: 53–57. 29. Schwinn MK, Machleidt T, Zimmerman K, Eggers CT, Dixon AS, Hurst R, Hall MP, Encell LP, Binkowski BF, Wood KV. 2018. CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol 13: 467–474. 34. Tetsuo M, Matsuno K, Tamura T, Fukuhara T, Kim T, Okamatsu M, Tautz N, Matsuura Y, Sakoda Y. 2020. Development of a high-throughput serum neutralization test using recombinant pestiviruses possessing a small reporter tag. Pathogens 9: 188. 30. Shen L, Li Y, Chen J, Li C, Huang J, Luo Y, Sun Y, Li S, Qiu HJ. 2014. Generation of a recombinant classical swine fever virus stably expressing the firefly luciferase gene for quantitative antiviral assay. Antiviral Res 109: 15–21. 26. Roehe PM, Woodward MJ, Edwards S. 1992. Characterisation of p20 gene sequences from a border disease-like pestivirus isolated from pigs. Vet Microbiol 33: 231–238. 14. Lee YM, Tscherne DM, Yun SI, Frolov I, Rice CM. 2005. Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication. J Virol 79: 3231–3242. 5. Ellenberg P, Linero FN, Scolaro LA. 2007. Superinfection exclusion in BHK-21 cells persistently infected with Junín virus. J Gen Virol 88: 2730–2739. 16. Meyers G, Thiel HJ. 1995. Cytopathogenicity of classical swine fever virus caused by defective interfering particles. J Virol 69: 3683–3689. 31. Tamura T, Igarashi M, Enkhbold B, Suzuki T, Okamatsu M, Ono C, Mori H, Izumi T, Sato A, Fauzyah Y, Okamoto T, Sakoda Y, Fukuhara T, Matsuura Y. 2019. In vivo dynamics of reporter Flaviviridae viruses. J Virol 93: e01191-19. 20. Mittelholzer C, Moser C, Tratschin JD, Hofmann MA. 1998. Porcine cells persistently infected with classical swine fever virus protected from pestivirus-induced cytopathic effect. J Gen Virol 79: 2981–2987. 10. Huang IC, Li W, Sui J, Marasco W, Choe H, Farzan M. 2008. Influenza A virus neuraminidase limits viral superinfection. J Virol 82: 4834–4843. 35. Tscherne DM, Evans MJ, von Hahn T, Jones CT, Stamataki Z, McKeating JA, Lindenbach BD, Rice CM. 2007. Superinfection exclusion in cells infected with hepatitis C virus. J Virol 81: 3693–3703. 2. Aoki H, Ishikawa K, Sakoda Y, Sekiguchi H, Kodama M, Suzuki S, Fukusho A. 2001. Characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic RNA isolated from wild boar. J Vet Med Sci 63: 751–758. 33. Tautz N, Thiel HJ, Dubovi EJ, Meyers G. 1994. Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion. J Virol 68: 3289–3297. 18. Meyers G, Tautz N, Stark R, Brownlie J, Dubovi EJ, Collett MS, Thiel HJ. 1992. Rearrangement of viral sequences in cytopathogenic pestiviruses. Virology 191: 368–386. 9. Howley P, Knipe D. 2021. Flaviviridae. pp. 245–301. In: Fields Virology, 7th ed., Wolters Kluwer, Philadelphia. 12. Itakura Y, Matsuno K, Ito A, Gerber M, Liniger M, Fujimoto Y, Tamura T, Kameyama KI, Okamatsu M, Ruggli N, Kida H, Sakoda Y. 2020. A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis. Virus Res 276: 197809–197809. 8. Horga MA, Gusella GL, Greengard O, Poltoratskaia N, Porotto M, Moscona A. 2000. Mechanism of interference mediated by human parainfluenza virus type 3 infection. J Virol 74: 11792–11799. 13. Kameyama K, Sakoda Y, Tamai K, Igarashi H, Tajima M, Mochizuki T, Namba Y, Kida H. 2006. Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen. J Virol Methods 138: 140–146. 23. Nagai M, Sakoda Y, Mori M, Hayashi M, Kida H, Akashi H. 2003. Insertion of cellular sequence and RNA recombination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus. J Gen Virol 84: 447–452. 7. Gillespie JH, Madin SH, Darby NB Jr 1962. Cellular resistance in tissue culture, induced by noncytopathogenic strains, to a cytopathogenic strain of virus diarrhea virus of cattle. Proc Soc Exp Biol Med 110: 248–250. 11. International Committee on Taxonomy of Viruses. 2022. Virus taxonomy: The ICVT report on virus classification and taxon nomenclature, Family: Flaviviridae Genus: Pestivirus. https://ictv.global/report/chapter/flaviviridaeport/flaviviridaeport/flaviviridae/pestivirus [accessed on January 4, 2024]. 24. National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries. 2023. The standard of the biological products for animals. https://www.maff.go.jp/nval/kijyun/seizaikijyun/index.html#3 [accessed on January 29, 2024]. 19. Michel N, Allespach I, Venzke S, Fackler OT, Keppler OT. 2005. The Nef protein of human immunodeficiency virus establishes superinfection immunity by a dual strategy to downregulate cell-surface CCR5 and CD4. Curr Biol 15: 714–723. 21. Moser C, Tratschin JD, Hofmann MA. 1998. A recombinant classical swine fever virus stably expresses a marker gene. J Virol 72: 5318–5322. 22. Muñoz-González S, Pérez-Simó M, Colom-Cadena A, Cabezón O, Bohórquez JA, Rosell R, Pérez LJ, Marco I, Lavín S, Domingo M, Ganges L. 2016. Classical swine fever virus vs. vlassical swine fever virus: the superinfection exclusion phenomenon in experimentally infected wild boar. PLoS One 11: e0149469. 4. Dixon AS, Schwinn MK, Hall MP, Zimmerman K, Otto P, Lubben TH, Butler BL, Binkowski BF, Machleidt T, Kirkland TA, Wood MG, Eggers CT, Encell LP, Wood KV. 2016. NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS Chem Biol 11: 400–408. 1. Adams RH, Brown DT. 1985. BHK cells expressing Sindbis virus-induced homologous interference allow the translation of nonstructural genes of superinfecting virus. J Virol 54: 351–357. 32. Tamura T, Fukuhara T, Uchida T, Ono C, Mori H, Sato A, Fauzyah Y, Okamoto T, Kurosu T, Setoh YX, Imamura M, Tautz N, Sakoda Y, Khromykh AA, Chayama K, Matsuura Y. 2018. Characterization of recombinant Flaviviridae viruses possessing a small reporter tag. J Virol 92: e01582-17. 25. Reed LJ, Muench H. 1938. A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27: 493–497. 22 23 24 25 26 27 28 29 30 31 10 32 11 33 12 34 13 35 14 36 15 16 17 18 19 1 2 3 4 5 6 7 8 9 20 21 |
| References_xml | – reference: 5. Ellenberg P, Linero FN, Scolaro LA. 2007. Superinfection exclusion in BHK-21 cells persistently infected with Junín virus. J Gen Virol 88: 2730–2739. – reference: 4. Dixon AS, Schwinn MK, Hall MP, Zimmerman K, Otto P, Lubben TH, Butler BL, Binkowski BF, Machleidt T, Kirkland TA, Wood MG, Eggers CT, Encell LP, Wood KV. 2016. NanoLuc complementation reporter optimized for accurate measurement of protein interactions in cells. ACS Chem Biol 11: 400–408. – reference: 16. Meyers G, Thiel HJ. 1995. Cytopathogenicity of classical swine fever virus caused by defective interfering particles. J Virol 69: 3683–3689. – reference: 9. Howley P, Knipe D. 2021. Flaviviridae. pp. 245–301. In: Fields Virology, 7th ed., Wolters Kluwer, Philadelphia. – reference: 20. Mittelholzer C, Moser C, Tratschin JD, Hofmann MA. 1998. Porcine cells persistently infected with classical swine fever virus protected from pestivirus-induced cytopathic effect. J Gen Virol 79: 2981–2987. – reference: 17. Meyers G, Rümenapf T, Thiel HJ. 1989. Ubiquitin in a togavirus. Nature 341: 491. – reference: 34. Tetsuo M, Matsuno K, Tamura T, Fukuhara T, Kim T, Okamatsu M, Tautz N, Matsuura Y, Sakoda Y. 2020. Development of a high-throughput serum neutralization test using recombinant pestiviruses possessing a small reporter tag. Pathogens 9: 188. – reference: 12. Itakura Y, Matsuno K, Ito A, Gerber M, Liniger M, Fujimoto Y, Tamura T, Kameyama KI, Okamatsu M, Ruggli N, Kida H, Sakoda Y. 2020. A cloned classical swine fever virus derived from the vaccine strain GPE- causes cytopathic effect in CPK-NS cells via type-I interferon-dependent necroptosis. Virus Res 276: 197809–197809. – reference: 26. Roehe PM, Woodward MJ, Edwards S. 1992. Characterisation of p20 gene sequences from a border disease-like pestivirus isolated from pigs. Vet Microbiol 33: 231–238. – reference: 27. Sakoda Y, Fukusho A. 1998. Establishment and characterization of a porcine kidney cell line, FS-L3, which forms unique multicellular domes in serum-free culture. In Vitro Cell Dev Biol Anim 34: 53–57. – reference: 33. Tautz N, Thiel HJ, Dubovi EJ, Meyers G. 1994. Pathogenesis of mucosal disease: a cytopathogenic pestivirus generated by an internal deletion. J Virol 68: 3289–3297. – reference: 3. Criddle A, Thornburg T, Kochetkova I, DePartee M, Taylor MP. 2016. gD-independent superinfection exclusion of alphaherpesviruses. J Virol 90: 4049–4058. – reference: 15. Li Y, Shen L, Sun Y, Yuan J, Huang J, Li C, Li S, Luo Y, Qiu HJ. 2013. Simplified serum neutralization test based on enhanced green fluorescent protein-tagged classical swine fever virus. J Clin Microbiol 51: 2710–2712. – reference: 28. Sakoda Y, Hikawa M, Tamura T, Fukusho A. 1998. Establishment of a serum-free culture cell line, CPK-NS, which is useful for assays of classical swine fever virus. J Virol Methods 75: 59–68. – reference: 30. Shen L, Li Y, Chen J, Li C, Huang J, Luo Y, Sun Y, Li S, Qiu HJ. 2014. Generation of a recombinant classical swine fever virus stably expressing the firefly luciferase gene for quantitative antiviral assay. Antiviral Res 109: 15–21. – reference: 8. Horga MA, Gusella GL, Greengard O, Poltoratskaia N, Porotto M, Moscona A. 2000. Mechanism of interference mediated by human parainfluenza virus type 3 infection. J Virol 74: 11792–11799. – reference: 22. Muñoz-González S, Pérez-Simó M, Colom-Cadena A, Cabezón O, Bohórquez JA, Rosell R, Pérez LJ, Marco I, Lavín S, Domingo M, Ganges L. 2016. Classical swine fever virus vs. vlassical swine fever virus: the superinfection exclusion phenomenon in experimentally infected wild boar. PLoS One 11: e0149469. – reference: 18. Meyers G, Tautz N, Stark R, Brownlie J, Dubovi EJ, Collett MS, Thiel HJ. 1992. Rearrangement of viral sequences in cytopathogenic pestiviruses. Virology 191: 368–386. – reference: 19. Michel N, Allespach I, Venzke S, Fackler OT, Keppler OT. 2005. The Nef protein of human immunodeficiency virus establishes superinfection immunity by a dual strategy to downregulate cell-surface CCR5 and CD4. Curr Biol 15: 714–723. – reference: 14. Lee YM, Tscherne DM, Yun SI, Frolov I, Rice CM. 2005. Dual mechanisms of pestiviral superinfection exclusion at entry and RNA replication. J Virol 79: 3231–3242. – reference: 7. Gillespie JH, Madin SH, Darby NB Jr 1962. Cellular resistance in tissue culture, induced by noncytopathogenic strains, to a cytopathogenic strain of virus diarrhea virus of cattle. Proc Soc Exp Biol Med 110: 248–250. – reference: 21. Moser C, Tratschin JD, Hofmann MA. 1998. A recombinant classical swine fever virus stably expresses a marker gene. J Virol 72: 5318–5322. – reference: 10. Huang IC, Li W, Sui J, Marasco W, Choe H, Farzan M. 2008. Influenza A virus neuraminidase limits viral superinfection. J Virol 82: 4834–4843. – reference: 36. Zou G, Zhang B, Lim PY, Yuan Z, Bernard KA, Shi PY. 2009. Exclusion of West Nile virus superinfection through RNA replication. J Virol 83: 11765–11776. – reference: 6. Geib T, Sauder C, Venturelli S, Hässler C, Staeheli P, Schwemmle M. 2003. Selective virus resistance conferred by expression of Borna disease virus nucleocapsid components. J Virol 77: 4283–4290. – reference: 35. Tscherne DM, Evans MJ, von Hahn T, Jones CT, Stamataki Z, McKeating JA, Lindenbach BD, Rice CM. 2007. Superinfection exclusion in cells infected with hepatitis C virus. J Virol 81: 3693–3703. – reference: 1. Adams RH, Brown DT. 1985. BHK cells expressing Sindbis virus-induced homologous interference allow the translation of nonstructural genes of superinfecting virus. J Virol 54: 351–357. – reference: 11. International Committee on Taxonomy of Viruses. 2022. Virus taxonomy: The ICVT report on virus classification and taxon nomenclature, Family: Flaviviridae Genus: Pestivirus. https://ictv.global/report/chapter/flaviviridaeport/flaviviridaeport/flaviviridae/pestivirus [accessed on January 4, 2024]. – reference: 31. Tamura T, Igarashi M, Enkhbold B, Suzuki T, Okamatsu M, Ono C, Mori H, Izumi T, Sato A, Fauzyah Y, Okamoto T, Sakoda Y, Fukuhara T, Matsuura Y. 2019. In vivo dynamics of reporter Flaviviridae viruses. J Virol 93: e01191-19. – reference: 24. National Veterinary Assay Laboratory, Ministry of Agriculture, Forestry and Fisheries. 2023. The standard of the biological products for animals. https://www.maff.go.jp/nval/kijyun/seizaikijyun/index.html#3 [accessed on January 29, 2024]. – reference: 29. Schwinn MK, Machleidt T, Zimmerman K, Eggers CT, Dixon AS, Hurst R, Hall MP, Encell LP, Binkowski BF, Wood KV. 2018. CRISPR-mediated tagging of endogenous proteins with a luminescent peptide. ACS Chem Biol 13: 467–474. – reference: 32. Tamura T, Fukuhara T, Uchida T, Ono C, Mori H, Sato A, Fauzyah Y, Okamoto T, Kurosu T, Setoh YX, Imamura M, Tautz N, Sakoda Y, Khromykh AA, Chayama K, Matsuura Y. 2018. Characterization of recombinant Flaviviridae viruses possessing a small reporter tag. J Virol 92: e01582-17. – reference: 13. Kameyama K, Sakoda Y, Tamai K, Igarashi H, Tajima M, Mochizuki T, Namba Y, Kida H. 2006. Development of an immunochromatographic test kit for rapid detection of bovine viral diarrhea virus antigen. J Virol Methods 138: 140–146. – reference: 23. Nagai M, Sakoda Y, Mori M, Hayashi M, Kida H, Akashi H. 2003. Insertion of cellular sequence and RNA recombination in the structural protein coding region of cytopathogenic bovine viral diarrhoea virus. J Gen Virol 84: 447–452. – reference: 25. Reed LJ, Muench H. 1938. A simple method of estimating fifty per cent endpoints. Am J Epidemiol 27: 493–497. – reference: 2. Aoki H, Ishikawa K, Sakoda Y, Sekiguchi H, Kodama M, Suzuki S, Fukusho A. 2001. Characterization of classical swine fever virus associated with defective interfering particles containing a cytopathogenic subgenomic RNA isolated from wild boar. J Vet Med Sci 63: 751–758. – ident: 31 doi: 10.1128/JVI.01191-19 – ident: 18 doi: 10.1016/0042-6822(92)90199-Y – ident: 16 doi: 10.1128/jvi.69.6.3683-3689.1995 – ident: 35 doi: 10.1128/JVI.01748-06 – ident: 6 doi: 10.1128/JVI.77.7.4283-4290.2003 – ident: 33 doi: 10.1128/jvi.68.5.3289-3297.1994 – ident: 36 doi: 10.1128/JVI.01205-09 – ident: 17 doi: 10.1038/341491a0 – ident: 3 doi: 10.1128/JVI.00089-16 – ident: 1 doi: 10.1128/jvi.54.2.351-357.1985 – ident: 24 – ident: 9 – ident: 13 doi: 10.1016/j.jviromet.2006.08.005 – ident: 20 doi: 10.1099/0022-1317-79-12-2981 – ident: 21 doi: 10.1128/JVI.72.6.5318-5322.1998 – ident: 26 doi: 10.1016/0378-1135(92)90051-T – ident: 15 doi: 10.1128/JCM.00859-13 – ident: 34 doi: 10.3390/pathogens9030188 – ident: 12 doi: 10.1016/j.virusres.2019.197809 – ident: 8 doi: 10.1128/JVI.74.24.11792-11799.2000 – ident: 11 – ident: 32 doi: 10.1128/JVI.01582-17 – ident: 30 doi: 10.1016/j.antiviral.2014.06.006 – ident: 4 doi: 10.1021/acschembio.5b00753 – ident: 28 doi: 10.1016/S0166-0934(98)00098-6 – ident: 29 doi: 10.1021/acschembio.7b00549 – ident: 22 doi: 10.1371/journal.pone.0149469 – ident: 27 doi: 10.1007/s11626-998-0053-6 – ident: 25 doi: 10.1093/oxfordjournals.aje.a118408 – ident: 5 doi: 10.1099/vir.0.83041-0 – ident: 7 doi: 10.3181/00379727-110-27481 – ident: 23 doi: 10.1099/vir.0.18773-0 – ident: 19 doi: 10.1016/j.cub.2005.02.058 – ident: 14 doi: 10.1128/JVI.79.6.3231-3242.2005 – ident: 2 doi: 10.1292/jvms.63.751 – ident: 10 doi: 10.1128/JVI.00079-08 |
| SSID | ssj0021469 |
| Score | 2.355001 |
| Snippet | Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their... Pestiviruses are classified into two biotypes based on their cytopathogenicity. As the majority of pestivirus field isolates are noncytopathogenic, their... |
| SourceID | pubmedcentral proquest pubmed crossref jstage |
| SourceType | Open Access Repository Aggregation Database Index Database Enrichment Source Publisher |
| StartPage | 389 |
| SubjectTerms | Animals Biotypes Border disease Cell culture classical swine fever virus Classical Swine Fever Virus - genetics Hog cholera infectivity titer luciferase activity Luciferases - genetics pestivirus Pestivirus - genetics Superinfection Superinfection - veterinary superinfection exclusion Swine Swine Diseases Titration Virology Viruses |
| Title | Establishment of a superinfection exclusion method for pestivirus titration using a recombinant reporter pestiviruses |
| URI | https://www.jstage.jst.go.jp/article/jvms/86/4/86_24-0005/_article/-char/en https://www.ncbi.nlm.nih.gov/pubmed/38355118 https://www.proquest.com/docview/3054725948 https://www.proquest.com/docview/2927213909 https://pubmed.ncbi.nlm.nih.gov/PMC11061576 |
| Volume | 86 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| ispartofPNX | Journal of Veterinary Medical Science, 2024, Vol.86(4), pp.389-395 |
| link | http://utb.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Nb9QwEB2VglAvCEqBQKlcqZyqlKxjJ44QQgi1Kq22py7qLbITpx_aZstmU5V_z0zsRN2qXLjk4pnNyh5n3vOMZwB2uNTZSBgdimSED6XiMFOJDiMrqjipVMoLIorjk-RwIo7O5NkK9N1G_QQ2j1I76ic1mU_37n7_-YYb_mtXGyHjn69ur5s9LuiCtHwCT9EnpdTLYCyGeAJ1r3ZV90ZJmKLX9ynwD7XX4DkyNkmoe8lPPbtCqHZuH0OhD5Mp73mng5fwwsNK9t3ZwStYsfU6rP-iXJfuwi0b-xj6a2j38S3d2RMdDLJZxTRrWip47POyambvimlLx2jMNZhmiGzZDdXjuL2ctw1DC3OGwyht_hx_gHj1temyapgLQ9j7GrbZgMnB_umPw9A3XwgLdNmLMNMCZwLBETeFsFrFVlZWV-i8kgJRTxoJY4yOi6iMS6UMGoJBLqnKqtTCcgR2b2C1ntX2HbAylRUCO06Xs4S2iBAkqpCGiQpZRgHs9lOdF74yOTXImObEUHCNclqjnAuKlMsAPg3SN64ixz_kvrhVG6T8XnRSKskFPbz0MEiX3fCLEcBmv9R5b5Q5fhsFWk8mVADbwzDuRwqy6NrOWnx7xpFUx1mUBfDWWcbwB3rbCkAt2cwgQLW-l0fqy4uu5veIqDtyw_f_r_oB1nhEhk7nR5uwupi39iMiqoXZQi7x83ir2zJ_AeWoJ2c |
| linkProvider | Scholars Portal |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Establishment+of+a+superinfection+exclusion+method+for+pestivirus+titration+using+a+recombinant+reporter+pestiviruses&rft.jtitle=Journal+of+veterinary+medical+science&rft.au=MIMURA%2C+Yume&rft.au=HIONO%2C+Takahiro&rft.au=HUYNH%2C+Loc+Tan&rft.au=OGINO%2C+Saho&rft.date=2024&rft.pub=The+Japanese+Society+of+Veterinary+Science&rft.issn=0916-7250&rft.eissn=1347-7439&rft.volume=86&rft.issue=4&rft.spage=389&rft.epage=395&rft_id=info:doi/10.1292%2Fjvms.24-0005&rft_id=info%3Apmid%2F38355118&rft.externalDocID=PMC11061576 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0916-7250&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0916-7250&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0916-7250&client=summon |