Cross-reactive and mono-reactive SARS-CoV-2 CD4+ T cells in prepandemic and COVID-19 convalescent individuals

• Published in: PLoS pathogens Vol. 17; no. 12; p. e1010203
• Main Authors: Johansson, Alexandra M., Malhotra, Uma, Kim, Yeseul G., Gomez, Rebecca, Krist, Maxwell P., Wald, Anna, Koelle, David M., Kwok, William W.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.12.2021; Public Library of Science (PLoS)
• Subjects: Alleles; Amino acid sequence; Amino acids; Antigenic determinants; Avidity; Biology and Life Sciences; CD4 antigen; CD4-Positive T-Lymphocytes - immunology; Cell lines; Convalescence; Coronaviruses; COVID-19; COVID-19 - epidemiology; COVID-19 - immunology; Cross Reactions; Cross-reactivity; Epidemiology; Epitopes; Epitopes, T-Lymphocyte - immunology; Experiments; Frequency analysis; Histocompatibility antigen HLA; Humans; Identification and classification; Influenza; Lymphocytes; Lymphocytes T; Medicine and Health Sciences; Nucleocapsids; Pandemics; Peptides; Peripheral blood mononuclear cells; Population genetics; Proteins; Reagents; Research and Analysis Methods; SARS-CoV-2 - immunology; Severe acute respiratory syndrome; Severe acute respiratory syndrome coronavirus 2; Spike Glycoprotein, Coronavirus - immunology; T cell receptors; T cells; Viral diseases; Viruses; World population; United States

Class II tetramer reagents for eleven common DR alleles and a DP allele prevalent in the world population were used to identify SARS-CoV-2 CD4+ T cell epitopes. A total of 112, 28 and 42 epitopes specific for Spike, Membrane and Nucleocapsid, respectively, with defined HLA-restriction were identifie...