Overexpression of SrDXS1 and SrKAH enhances steviol glycosides content in transgenic Stevia plants

• Published in: BMC plant biology Vol. 19; no. 1; p. 1
• Main Authors: Zheng, Junshi, Zhuang, Yan, Mao, Hui-Zhu, Jang, In-Cheol
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 03.01.2019; BioMed Central; BMC
• Subjects: 1-deoxy-d-xylulose-5-phosphate synthase 1; Acids; Biosynthesis; Botanical research; Breeding; Diterpenes, Kaurane - metabolism; Fluorescence; Gene expression; Genetic aspects; Genetic engineering; Genetic Engineering - methods; Genetic research; Genetic transformation; Glucosides - metabolism; Glycosides; Green fluorescent protein; Hydroxylase; Kaurenoic acid hydroxylase; Metabolic engineering; Metabolism; Methods; Mixed Function Oxygenases - genetics; Mixed Function Oxygenases - metabolism; Monosaccharides; Neomycin; Phosphates; Physiological aspects; Plant Leaves - metabolism; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Shoots - metabolism; Plants (botany); Plants, Genetically Modified; Proteins; Protocol; Regeneration; Shoots; Stevia; Stevia - enzymology; Stevia - genetics; Stevia - metabolism; Stevia transformation; Steviol glycosides; Sweeteners; Transferases - genetics; Transferases - metabolism; Transgenic plants; Transgenic Stevia; Xylulose; Stevia transformation; Metabolic engineering; Transgenic Stevia; 1-deoxy-d-xylulose-5-phosphate synthase 1; Kaurenoic acid hydroxylase; Steviol glycosides

Stevia rebaudiana produces sweet-tasting steviol glycosides (SGs) in its leaves which can be used as natural sweeteners. Metabolic engineering of Stevia would offer an alternative approach to conventional breeding for enhanced production of SGs. However, an effective protocol for Stevia transformati...