Disruption of gul-1 decreased the culture viscosity and improved protein secretion in the filamentous fungus Neurospora crassa

• Published in: Microbial cell factories Vol. 17; no. 1; p. 96
• Main Authors: Lin, Liangcai, Sun, Zhiyong, Li, Jingen, Chen, Yong, Liu, Qian, Sun, Wenliang, Tian, Chaoguang
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 16.06.2018; BioMed Central; BMC
• Subjects: Analysis; Aspergillus fumigatus; Binding proteins; Biofuels; Cell culture; Cell walls; Cellulase; Cellulolytic fungi; Clonal deletion; Cultivation; Cytology; Ethanol; Fermentation; Fungal Proteins - genetics; Fungi; Gene expression; Genes; Genetic aspects; Genetic engineering; Genetic modification; Genomes; Genomics; Messenger RNA; Morphogenesis; Morphology; Mutagenesis; Mutants; Mycelia; Mycelial morphology; Mycelium; Neurospora; Neurospora crassa; Neurospora crassa - genetics; Overexpression; Pellet; Physical factors; Protein secretion; Proteins; Saccharomyces cerevisiae; Secretion; Transcription; Transcription (Genetics); Viscosity; Yeast; Viscosity; Mycelial morphology; Pellet; Protein secretion; Neurospora crassa

The cellulolytic fungus Neurospora crassa is considered a potential host for enzyme and bioethanol production. However, large scale applications are hindered by its filamentous growth. Although previous investigations have shown that mycelial morphology in submerged culture can be controlled by alte...