Mechanism of DNA Methylation-Directed Histone Methylation by KRYPTONITE

• Published in: Molecular cell Vol. 55; no. 3; pp. 495 - 504
• Main Authors: Du, Jiamu, Johnson, Lianna M., Groth, Martin, Feng, Suhua, Hale, Christopher J., Li, Sisi, Vashisht, Ajay A., Gallego-Bartolome, Javier, Wohlschlegel, James A., Patel, Dinshaw J., Jacobsen, Steven E.
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 07.08.2014
• Subjects: Arabidopsis; Arabidopsis - chemistry; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - chemistry; Arabidopsis Proteins - metabolism; Binding Sites - genetics; DNA; DNA Methylation; DNA, Plant - chemistry; DNA, Plant - genetics; Epigenesis, Genetic; Gene Expression Regulation, Plant; Genome, Plant; Histone-Lysine N-Methyltransferase - chemistry; Histone-Lysine N-Methyltransferase - metabolism; histones; Histones - physiology; methyltransferases; Models, Molecular; mutants; S-Adenosylhomocysteine - metabolism; X-Ray Diffraction

In Arabidopsis, CHG DNA methylation is controlled by the H3K9 methylation mark through a self-reinforcing loop between DNA methyltransferase CHROMOMETHYLASE3 (CMT3) and H3K9 histone methyltransferase KRYPTONITE/SUVH4 (KYP). We report on the structure of KYP in complex with methylated DNA, substrate...